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Western blot
Blocking/NeutralizingAntibody data
- Antibody Data
- Antigen structure
- References [12]
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- Validations
- Western blot [2]
- Immunocytochemistry [1]
- Immunohistochemistry [1]
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- Product number
- GTX13939 - Provider product page
- Provider
- GeneTex
- Proper citation
- GeneTex Cat#GTX13939, RRID:AB_369391
- Product name
- CENPA antibody [3-19]
- Antibody type
- Monoclonal
- Reactivity
- Human, Chicken/Avian
- Host
- Mouse
Submitted references Reconstitution of a 26-Subunit Human Kinetochore Reveals Cooperative Microtubule Binding by CENP-OPQUR and NDC80.
The ESCRT protein Chmp4c regulates mitotic spindle checkpoint signaling.
hDNA2 nuclease/helicase promotes centromeric DNA replication and genome stability.
Chmp4c is required for stable kinetochore-microtubule attachments.
Insights from biochemical reconstitution into the architecture of human kinetochores.
Clks 1, 2 and 4 prevent chromatin breakage by regulating the Aurora B-dependent abscission checkpoint.
DNA damage response and spindle assembly checkpoint function throughout the cell cycle to ensure genomic integrity.
Chk1 protects against chromatin bridges by constitutively phosphorylating BLM serine 502 to inhibit BLM degradation.
PUL21a-Cyclin A2 interaction is required to protect human cytomegalovirus-infected cells from the deleterious consequences of mitotic entry.
Chk2 prevents mitotic exit when the majority of kinetochores are unattached.
Chk1 and Mps1 jointly regulate correction of merotelic kinetochore attachments.
CENP-A reduction induces a p53-dependent cellular senescence response to protect cells from executing defective mitoses.
Pesenti ME, Prumbaum D, Auckland P, Smith CM, Faesen AC, Petrovic A, Erent M, Maffini S, Pentakota S, Weir JR, Lin YC, Raunser S, McAinsh AD, Musacchio A
Molecular cell 2018 Sep 20;71(6):923-939.e10
Molecular cell 2018 Sep 20;71(6):923-939.e10
The ESCRT protein Chmp4c regulates mitotic spindle checkpoint signaling.
Petsalaki E, Dandoulaki M, Zachos G
The Journal of cell biology 2018 Mar 5;217(3):861-876
The Journal of cell biology 2018 Mar 5;217(3):861-876
hDNA2 nuclease/helicase promotes centromeric DNA replication and genome stability.
Li Z, Liu B, Jin W, Wu X, Zhou M, Liu VZ, Goel A, Shen Z, Zheng L, Shen B
The EMBO journal 2018 Jul 13;37(14)
The EMBO journal 2018 Jul 13;37(14)
Chmp4c is required for stable kinetochore-microtubule attachments.
Petsalaki E, Dandoulaki M, Zachos G
Chromosoma 2018 Dec;127(4):461-473
Chromosoma 2018 Dec;127(4):461-473
Insights from biochemical reconstitution into the architecture of human kinetochores.
Weir JR, Faesen AC, Klare K, Petrovic A, Basilico F, Fischböck J, Pentakota S, Keller J, Pesenti ME, Pan D, Vogt D, Wohlgemuth S, Herzog F, Musacchio A
Nature 2016 Sep 8;537(7619):249-253
Nature 2016 Sep 8;537(7619):249-253
Clks 1, 2 and 4 prevent chromatin breakage by regulating the Aurora B-dependent abscission checkpoint.
Petsalaki E, Zachos G
Nature communications 2016 Apr 29;7:11451
Nature communications 2016 Apr 29;7:11451
DNA damage response and spindle assembly checkpoint function throughout the cell cycle to ensure genomic integrity.
Lawrence KS, Chau T, Engebrecht J
PLoS genetics 2015 Apr;11(4):e1005150
PLoS genetics 2015 Apr;11(4):e1005150
Chk1 protects against chromatin bridges by constitutively phosphorylating BLM serine 502 to inhibit BLM degradation.
Petsalaki E, Dandoulaki M, Morrice N, Zachos G
Journal of cell science 2014 Sep 15;127(Pt 18):3902-8
Journal of cell science 2014 Sep 15;127(Pt 18):3902-8
PUL21a-Cyclin A2 interaction is required to protect human cytomegalovirus-infected cells from the deleterious consequences of mitotic entry.
Eifler M, Uecker R, Weisbach H, Bogdanow B, Richter E, König L, Vetter B, Lenac-Rovis T, Jonjic S, Neitzel H, Hagemeier C, Wiebusch L
PLoS pathogens 2014 Oct;10(10):e1004514
PLoS pathogens 2014 Oct;10(10):e1004514
Chk2 prevents mitotic exit when the majority of kinetochores are unattached.
Petsalaki E, Zachos G
The Journal of cell biology 2014 May 12;205(3):339-56
The Journal of cell biology 2014 May 12;205(3):339-56
Chk1 and Mps1 jointly regulate correction of merotelic kinetochore attachments.
Petsalaki E, Zachos G
Journal of cell science 2013 Mar 1;126(Pt 5):1235-46
Journal of cell science 2013 Mar 1;126(Pt 5):1235-46
CENP-A reduction induces a p53-dependent cellular senescence response to protect cells from executing defective mitoses.
Maehara K, Takahashi K, Saitoh S
Molecular and cellular biology 2010 May;30(9):2090-104
Molecular and cellular biology 2010 May;30(9):2090-104
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Supportive validation
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- Hela cells (human), Friend cells (mouse) and calf thymus (Roche). 5 g acid-extracted histones were loaded per lane. Images taken following 5 min exposure. 1:1000 dilution. Lane 1: Friend cells (mouse); Lane 2: Hela cells (human); Lane 3: Calf thymus. This
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- Western blot analysis of Jurkat (1), Raji (2), PC12 (3), Rat-1 (4), WR19L (5), C2C12 (6), and NIH3T3 (7) cell lysates probed with CENP-A mAb
Supportive validation
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- This picture shows human metaphase chromosomes detected with GTX13939 as primary antibody and AF488 goat anti-mouse secondary antibody (green). This image was kindly supplied as part of the review submitted by Professor Beth A. Sullivan, Boston University
Supportive validation
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- Immunohistochemical detection of CENP-A on paraffin embedded section of a squamous epithelium of human tonsil.
