15518-1-AP

antibody from Proteintech Group

Targeting: GLB1 EBP, ELNR1

Provider product page for 15518-1-AP

Western blot

ELISA

Immunocytochemistry

Immunohistochemistry

Antibody data

Antibody Data
Antigen structure
References [57]
Comments [0]
Validations
Western blot [1]
Immunocytochemistry [1]

Submit

Product number: 15518-1-AP - Provider product page
Provider: Proteintech Group
Proper citation: Proteintech Cat#15518-1-AP, RRID:AB_2263448
Product name: Beta Galactosidase antibody
Antibody type: Polyclonal
Description: Beta Galactosidase antibody (Cat. #15518-1-AP) is a rabbit polyclonal antibody that shows reactivity with human, mouse and has been validated for the following applications: IF, IHC, WB,ELISA.
Reactivity: Human, Mouse
Host: Rabbit
Conjugate: Unconjugated
Isotype: IgG
Vial size: 20ul, 150ul

GLB1 protein structure - 15518-1-AP shown in red.

Submitted references Resveratrol Mitigates Age-Associated Cognitive Decline via Inhibition of cGAS-STING-Mediated Microglial Senescence.

Duan X, Cheng J, Wang J, Chen W, Ruan Z
Cells 2026 Mar 16;15(6)

GABA-Induced Exosomes Improve Memory Impairment in Aged Mice.

Akama Y, Maeda S, Udono M, Nakamura U, Yamashita Y, Kim Y, Shirouchi B, Teruya K, Katakura Y
International journal of molecular sciences 2026 Mar 10;27(6)

Sympathetic overactivity-induced type H endothelial cell senescence contributes to the impairment of vascularized osteogenesis by PKM2-mediated glycolysis in preclinical stages of Alzheimer's mice.

Zhang W, Liu T, Rong X, Liu H, Li M
Bone 2026 Jun;207:117859

Aging-dependent expression of vascular endothelial growth factor receptor-1 is associated with T-cell immune senescence in carotid atherosclerosis.

Zhang X, Wu S, Lu T, Li J, Cui S, Liu Z, Xie W, Xu R, Wang Y, Li W, Jiao L, Wang T
Brain circulation 2026 Jan-Mar;12(1):31-41

Promoting the nuclear retention of HMGB1 attenuates astrocyte senescence.

Zhang N, Yang F, Shi J, Tang X, Fan A, Li W, Zhang F, Chai S, Chen Q, Wang C, Xu Y, Gong F, Lu Y, Zheng F
Journal of neuroinflammation 2026 Jan 12;23(1):55

The AHCY-adenosine complex rewires mRNA methylation to enhance fatty acid biosynthesis and tumorigenesis.

Liao K, Cao F, Wei C, Qian ZY, Hu HR, Pan WF, Feng ZQ, Lian SM, Xiao ZX, Sheng H, Mo HY, Zhao YX, Wu QN, Zeng ZL, Li B, Xu RH, Ju HQ
Cell research 2026 Feb;36(2):152-172

Impaired stemness in aging periodontal ligament stem cells is mediated by the progerin/endoplasmic reticulum stress/p53 axis.

Zhang X, Wang Y, Wang J, Zhang Y, Li R, Wang X, Ge X, Ye Q, Ji J, Fei D, Wang Q
Journal of advanced research 2025 Sep;75:377-388

Efficacy and safety of umbilical cord-derived mesenchymal stem cell-conditioned media for preventing and treating skin aging.

Ahn H, Han HS, Lee KH
World journal of stem cells 2025 Sep 26;17(9):108049

CTRP9 as a myokine mitigates sarcopenia via the LAMP-2A/NLRP3 pathway.

Li L, Zuo A, Yin R, Liu Q, Liu C, Li N, Xu D, Zhang S, Li J, Lei S, Ruan S, Li T, Guo Y
Cell death & disease 2025 Oct 7;16(1):710

Gestational exposure to nanoplastics disrupts fetal development by promoting the placental aging via ferroptosis of syncytiotrophoblast.

Chen Z, Zheng M, Wan T, Li J, Yuan X, Qin L, Zhang L, Hou T, Liu C, Li R
Environment international 2025 Mar;197:109361

Astrocytic HSP60 Deletion Induced Astrocyte Senescence and Inhibited Neuroregeneration via Modulating the S1P/Truncated-BDNF Pathway.

Zhu W, Cheng Y, Lang Z, Li W, Wei X
Journal of neuroscience research 2025 Jun;103(6):e70054

Microglial STING is a central safeguard against neurological decline with age.

Sulka KB, Carroll KA, Sawden M, Hopkins JW, Smolgovsky SA, Bayer AL, Reed E, Tai A, Alcaide P, Haydon P, Sharma S
Cell reports 2025 Jun 24;44(6):115749

ZNF451 collaborates with RNF8 to regulate RNF168 localization and amplify ubiquitination signaling to promote DNA damage repair and regulate radiosensitivity.

Xu F, Xia Q, Chen B, Wang R, Zhang J, Zhao X, Zhang Z, Yao Z, Zhang J, Zhou S, Li X, Chen B, Xu A, Wu L, Zhao G
Cell death and differentiation 2025 Jul;32(7):1303-1316

Involvement of metformin and aging in salivary expression of ACE2 and TMPRSS2.

Shikama Y, Otsuka K, Shikama Y, Furukawa M, Ishimaru N, Matsushita K
BioFactors (Oxford, England) 2025 Jan-Feb;51(1):e2154

CB-MNCs@ CS/HEC/GP promote wound healing in aged murine pressure ulcer model.

Yang ZC, Lin H, Liu GJ, Pan H, Zhu JL, Zhang XH, Gao F, Wang Z, Wang ZH
Stem cell research & therapy 2025 Feb 7;16(1):52

Targeting cellular senescence in dystrophin(-/-)/utrophin(-/-)double knockout mice improves musculoskeletal health and increases lifespan.

Gao X, Ruzbarsky JJ, Huard M, Sajedi SAH, Shyu PT, Xiao Z, Force BS, White S, Ayers J, Wang B, Guo XE, Huard J
Pharmacological research 2025 Dec;222:108016

Hesperidin Reverses Oxidative Stress-Induced Damage in Kidney Cells by Modulating Antioxidant, Longevity, and Senescence-Related Genes.

Buakaew S, Sakonsinsiri C, Lert-Itthiporn W, Cha'on U, Rudtanatip T, Kraiklang R, Kaewlert W, Rattanaseth P, Pakdeechote P, Thanan R
Biomedicines 2025 Dec 9;13(12)

PQBP3 prevents senescence by suppressing PSME3-mediated proteasomal Lamin B1 degradation.

Yoshioka Y, Huang Y, Jin X, Ngo KX, Kumaki T, Jin M, Toyoda S, Takayama S, Inotsume M, Fujita K, Homma H, Ando T, Tanaka H, Okazawa H
The EMBO journal 2024 Sep;43(18):3968-3999

The role and mechanism of engineered nanovesicles derived from hair follicle mesenchymal stem cells in the treatment of UVB-induced skin photoaging.

Jiang Z, Cheng H, Qian X, Tu J, Fan C, Pan Y, Lin Z, Chen J, Wang X, Zhang J
Journal of cosmetic dermatology 2024 Sep;23(9):3005-3020

STING1-accelerated vascular smooth muscle cell senescence-associated vascular calcification in diabetes is ameliorated by oleoylethanolamide via improved mitochondrial DNA oxidative damage.

Chen Z, Li X, Sun X, Xiao S, Chen T, Ren L, Liu N
Free radical biology & medicine 2024 Sep;222:437-455

Human adipose and umbilical cord mesenchymal stem cell-derived extracellular vesicles mitigate photoaging via TIMP1/Notch1.

Zhang H, Xiao X, Wang L, Shi X, Fu N, Wang S, Zhao RC
Signal transduction and targeted therapy 2024 Oct 30;9(1):294

P16(INK4a) deletion alleviates contrast-induced acute kidney injury by ameliorating renal cell apoptosis and suppressing inflammation and oxidative stress.

Zhang X, Huang G, Zhang Z, Wang F, Liu Q, Du Y, Wang X, Gu X
Experimental gerontology 2024 Mar;187:112372

Upregulation of CD8(+) regulatory T cells following liver-directed AAV gene therapy.

Gaddie CD, Senior KG, Chan C, Hoffman BE, Keeler GD
Cellular immunology 2024 Mar-Apr;397-398:104806

Early Pulmonary Fibrosis-like Changes in the Setting of Heat Exposure: DNA Damage and Cell Senescence.

Hou T, Zhang J, Wang Y, Zhang G, Li S, Fan W, Li R, Sun Q, Liu C
International journal of molecular sciences 2024 Mar 5;25(5)

PM(2.5)-induced cellular senescence drives brown adipose tissue impairment in middle-aged mice.

Hu R, Fan W, Li S, Zhang G, Zang L, Qin L, Li R, Chen R, Zhang L, Gu W, Zhang Y, Rajagopalan S, Sun Q, Liu C
Ecotoxicology and environmental safety 2024 Jun 15;278:116423

Machine Learning-Enhanced Estimation of Cellular Protein Levels from Bright-Field Images.

Tohgasaki T, Touyama A, Kousai S, Imai K
Bioengineering (Basel, Switzerland) 2024 Jul 31;11(8)

Ultrasound Stimulation of Piezoelectric Nanocomposite Hydrogels Boosts Chondrogenic Differentiation in Vitro, in Both a Normal and Inflammatory Milieu.

Ricotti L, Cafarelli A, Manferdini C, Trucco D, Vannozzi L, Gabusi E, Fontana F, Dolzani P, Saleh Y, Lenzi E, Columbaro M, Piazzi M, Bertacchini J, Aliperta A, Cain M, Gemmi M, Parlanti P, Jost C, Fedutik Y, Nessim GD, Telkhozhayeva M, Teblum E, Dumont E, Delbaldo C, Codispoti G, Martini L, Tschon M, Fini M, Lisignoli G
ACS nano 2024 Jan 23;18(3):2047-2065

HIF-1α/m(6)A/NF-κB/CCL3 axis-mediated immunosurveillance participates in low level benzene-related erythrohematopoietic development toxicity.

Cong X, Li X, Xu K, Yin L, Liang G, Sun R, Pu Y, Zhang J
Environment international 2024 Feb;184:108493

Fluorogenic Labeling Probe for the Imaging of Endogenous β-Galactosidase Activity in Cancer and Senescent Cells.

Wang JB, Zhao XR, Hu XL, Zang Y, Li J, He XP
ACS applied materials & interfaces 2024 Dec 18;16(50):68918-68927

Prosaposin maintains lipid homeostasis in dopamine neurons and counteracts experimental parkinsonism in rodents.

He Y, Kaya I, Shariatgorji R, Lundkvist J, Wahlberg LU, Nilsson A, Mamula D, Kehr J, Zareba-Paslawska J, Biverstål H, Chergui K, Zhang X, Andren PE, Svenningsson P
Nature communications 2023 Sep 19;14(1):5804

Inhibition of Indoxyl Sulfate-Induced Reactive Oxygen Species-Related Ferroptosis Alleviates Renal Cell Injury In Vitro and Chronic Kidney Disease Progression In Vivo.

Tsai LT, Weng TI, Chang TY, Lan KC, Chiang CK, Liu SH
Antioxidants (Basel, Switzerland) 2023 Oct 30;12(11)

Integrin β3-Mediated Cell Senescence Associates with Gut Inflammation and Intestinal Degeneration in Models of Alzheimer's Disease.

Tun X, Wang EJ, Gao Z, Lundberg K, Xu R, Hu D
International journal of molecular sciences 2023 Mar 16;24(6)

The Expression of αvβ3 and Osteopontin in Osteoarthritic Knee Cartilage and Their Correlations With Disease Severity and Chondrocyte Senescence.

Cheng C, Tian J, Gao SG, Zhou ZH, Yang RQ, Xiao K, Guo W, Liu L, Yang H, Zhang FJ
Applied immunohistochemistry & molecular morphology : AIMM 2023 Jan 1;31(1):57-63

Gene Editing Corrects In Vitro a G > A GLB1 Transition from a GM1 Gangliosidosis Patient.

Leclerc D, Goujon L, Jaillard S, Nouyou B, Cluzeau L, Damaj L, Dubourg C, Etcheverry A, Levade T, Froissart R, Dréano S, Guillory X, Eriksson LA, Launay E, Mouriaux F, Belaud-Rotureau MA, Odent S, Gilot D
The CRISPR journal 2023 Feb;6(1):17-31

Schwann Cells Accelerate Osteogenesis via the Mif/CD74/FOXO1 Signaling Pathway In Vitro.

Li JQ, Jiang HJ, Su XY, Feng L, Zhan NZ, Li SS, Chen ZJ, Chang BH, Cheng PZ, Yang L, Pei GX
Stem cells international 2022;2022:4363632

p16(INK4a) Plays Critical Role in Exacerbating Inflammaging in High Fat Diet Induced Skin.

Liang Y, Gu T, Peng S, Lin Y, Liu J, Wang X, Huang X, Zhang X, Zhu J, Zhao L, Fan C, Wang G, Gu X, Lin J
Oxidative medicine and cellular longevity 2022;2022:3415528

Senescent Phenotype of Astrocytes Leads to Activation of BV2 Microglia and N2a Neuronal Cells Death.

Zhang W, Yang X, Liu J, Pan Y, Zhang M, Chen L
Molecules (Basel, Switzerland) 2022 Sep 12;27(18)

Deficiency of the frontotemporal dementia gene GRN results in gangliosidosis.

Boland S, Swarup S, Ambaw YA, Malia PC, Richards RC, Fischer AW, Singh S, Aggarwal G, Spina S, Nana AL, Grinberg LT, Seeley WW, Surma MA, Klose C, Paulo JA, Nguyen AD, Harper JW, Walther TC, Farese RV Jr
Nature communications 2022 Oct 7;13(1):5924

TMT-based quantitative N-glycoproteomic analysis reveals glycoprotein protection can improve the quality of frozen bovine sperm.

Wang N, Yang M, He D, Li X, Zhang X, Han B, Liu C, Hai C, Li G, Zhao Y
International journal of biological macromolecules 2022 Oct 1;218:168-180

The fiber diameter traits of Tibetan cashmere goats are governed by the inherent differences in stress, hypoxic, and metabolic adaptations: an integrative study of proteome and transcriptome.

Zhao B, Wu C, Sammad A, Ma Z, Suo L, Wu Y, Fu X
BMC genomics 2022 Mar 7;23(1):191

Liver glycogen phosphorylase is upregulated in glioblastoma and provides a metabolic vulnerability to high dose radiation.

Zois CE, Hendriks AM, Haider S, Pires E, Bridges E, Kalamida D, Voukantsis D, Lagerholm BC, Fehrmann RSN, den Dunnen WFA, Tarasov AI, Baba O, Morris J, Buffa FM, McCullagh JSO, Jalving M, Harris AL
Cell death & disease 2022 Jun 28;13(6):573

Heat shock protein A12B gene therapy improves perfusion, promotes neovascularization, and decreases fibrosis in a murine model of hind limb ischemia.

Thirunavukkarasu M, Tipu Rishi M, Pradeep SR, Swaminathan S, Accorsi D, Palesty JA, Maulik N
Surgery 2021 Sep;170(3):969-977

Astrocytic microdomains from mouse cortex gain molecular control over long-term information storage and memory retention.

Vignoli B, Sansevero G, Sasi M, Rimondini R, Blum R, Bonaldo V, Biasini E, Santi S, Berardi N, Lu B, Canossa M
Communications biology 2021 Oct 5;4(1):1152

OPN Deficiency Increases the Severity of Osteoarthritis Associated with Aberrant Chondrocyte Senescence and Apoptosis and Upregulates the Expression of Osteoarthritis-Associated Genes.

Tian J, Cheng C, Kuang SD, Su C, Zhao X, Xiong YL, Li YS, Gao SG
Pain research & management 2020;2020:3428587

Herb-sourced emodin inhibits angiogenesis of breast cancer by targeting VEGFA transcription.

Zou G, Zhang X, Wang L, Li X, Xie T, Zhao J, Yan J, Wang L, Ye H, Jiao S, Xiang R, Shi Y
Theranostics 2020;10(15):6839-6853

Senescence Induced by BMI1 Inhibition Is a Therapeutic Vulnerability in H3K27M-Mutant DIPG.

Balakrishnan I, Danis E, Pierce A, Madhavan K, Wang D, Dahl N, Sanford B, Birks DK, Davidson N, Metselaar DS, Meel MH, Lemma R, Donson A, Vijmasi T, Katagi H, Sola I, Fosmire S, Alimova I, Steiner J, Gilani A, Hulleman E, Serkova NJ, Hashizume R, Hawkins C, Carcaboso AM, Gupta N, Monje M, Jabado N, Jones K, Foreman N, Green A, Vibhakar R, Venkataraman S
Cell reports 2020 Oct 20;33(3):108286

Legumain-deficient macrophages promote senescence of tumor cells by sustaining JAK1/STAT1 activation.

Shen L, Kang L, Wang D, Xun J, Chen C, Du L, Zhang M, Gong J, Mi X, Yue S, Zhang Y, Song X, Xiang R, Zhang Z, Tan X
Cancer letters 2020 Mar 1;472:40-49

Extracellular vesicles from adipose-derived stem cells ameliorate ultraviolet B-induced skin photoaging by attenuating reactive oxygen species production and inflammation.

Xu P, Xin Y, Zhang Z, Zou X, Xue K, Zhang H, Zhang W, Liu K
Stem cell research & therapy 2020 Jul 1;11(1):264

Spermidine alleviates cardiac aging by improving mitochondrial biogenesis and function.

Wang J, Li S, Wang J, Wu F, Chen Y, Zhang H, Guo Y, Lin Y, Li L, Yu X, Liu T, Zhao Y
Aging 2020 Jan 6;12(1):650-671

Sarcopenia is attenuated by TRB3 knockout in aging mice via the alleviation of atrophy and fibrosis of skeletal muscles.

Shang GK, Han L, Wang ZH, Liu YP, Yan SB, Sai WW, Wang D, Li YH, Zhang W, Zhong M
Journal of cachexia, sarcopenia and muscle 2020 Aug;11(4):1104-1120

Galactose-modified duocarmycin prodrugs as senolytics.

Guerrero A, Guiho R, Herranz N, Uren A, Withers DJ, Martínez-Barbera JP, Tietze LF, Gil J
Aging cell 2020 Apr;19(4):e13133

Seryl tRNA synthetase cooperates with POT1 to regulate telomere length and cellular senescence.

Li Y, Li X, Cao M, Jiang Y, Yan J, Liu Z, Yang R, Chen X, Sun P, Xiang R, Wang L, Shi Y
Signal transduction and targeted therapy 2019;4:50

Cardiac glycosides are broad-spectrum senolytics.

Guerrero A, Herranz N, Sun B, Wagner V, Gallage S, Guiho R, Wolter K, Pombo J, Irvine EE, Innes AJ, Birch J, Glegola J, Manshaei S, Heide D, Dharmalingam G, Harbig J, Olona A, Behmoaras J, Dauch D, Uren AG, Zender L, Vernia S, Martínez-Barbera JP, Heikenwalder M, Withers DJ, Gil J
Nature metabolism 2019 Nov;1(11):1074-1088

Active PKG II inhibited the growth and migration of ovarian cancer cells through blocking Raf/MEK and PI3K/Akt signaling pathways.

Wu Y, Cai Q, Li W, Cai Z, Liu Y, Li H, Pang J, Chen Y
Bioscience reports 2019 Aug 30;39(8)

β1-integrin is essential for vasoregulation and smooth muscle survival in vivo.

Turlo KA, Scapa J, Bagher P, Jones AW, Feil R, Korthuis RJ, Segal SS, Iruela-Arispe ML
Arteriosclerosis, thrombosis, and vascular biology 2013 Oct;33(10):2325-35

Unhealthy diet and ultrafine carbon black particles induce senescence and disease associated phenotypic changes.

Büchner N, Ale-Agha N, Jakob S, Sydlik U, Kunze K, Unfried K, Altschmied J, Haendeler J
Experimental gerontology 2013 Jan;48(1):8-16

Pioglitazone activates aortic telomerase and prevents stress-induced endothelial apoptosis.

Werner C, Gensch C, Pöss J, Haendeler J, Böhm M, Laufs U
Atherosclerosis 2011 May;216(1):23-34

No comments: Submit comment

Supportive validation

Submitted by: Proteintech Group (provider)
Main image
Experimental details: SH-SY5Y cells were subjected to SDS PAGE followed by western blot with 15518-1-AP(GLB1 antibody) at dilution of 1:400
Sample type: cell line

Supportive validation

Submitted by: Proteintech Group (provider)
Main image
Experimental details: Immunofluorescent analysis of Hela cells, using GLB1 antibody 15518-1-AP at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red).
Sample type: cell line