TA505442

antibody from OriGene

Targeting: GLB1 EBP, ELNR1

Western blot (Supportive data in Antibodypedia)

Immunohistochemistry (Supportive data in Antibodypedia)

Antibody Data

Product number

TA505442

Provider

OriGene

Proper citation

OriGene Cat#TA505442, RRID:AB_2623094

Product name

GLB1 mouse monoclonal antibody, clone OTI1E10 (formerly 1E10)

Antibody type

Monoclonal

Description

GLB1 mouse monoclonal antibody, clone OTI1E10 (formerly 1E10)

Host

Mouse

Conjugate

Unconjugated

Epitope

GLB1

Isotype

IgG

Antibody clone number

OTI1E10

Vial size

100 µl

Concentration

1 mg/ml

Western blot

Supportive validation

Submitted by

OriGene (provider)

Main image

Experimental details

Western blot analysis of extracts (35ug) from 9 different cell lines by using anti-GLB1 monoclonal antibody (HepG2: human; HeLa: human; SVT2: mouse; A549: human; COS7: monkey; Jurkat: human; MDCK: canine; PC12: rat; MCF7: human).

Validation comment

WB

Supportive validation

Submitted by

OriGene (provider)

Main image

Experimental details

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY GLB1 (RC200721, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-GLB1.

Validation comment

WB

Immunohistochemistry

Supportive validation

Submitted by

OriGene (provider)

Main image

Experimental details

Immunohistochemical staining of paraffin-embedded Carcinoma of Human thyroid tissue using anti-GLB1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min, TA505442)

Validation comment

IHC