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Western blotAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [4]
- Immunocytochemistry [2]
- Immunoprecipitation [1]
- Immunohistochemistry [4]
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- Product number
- GTX114439 - Provider product page
- Provider
- GeneTex
- Proper citation
- GeneTex Cat#GTX114439, RRID:AB_10620804
- Product name
- GNAO1 antibody
- Antibody type
- Polyclonal
- Reactivity
- Human, Mouse, Rat
- Host
- Rabbit
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Supportive validation
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- GeneTex (provider)
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- Experimental details
- Sample (30 ?g of whole cell lysate) A: HeLa B: NT2D1 10% SDS PAGE GTX114439 diluted at 1:1000 The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
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- GeneTex (provider)
- Main image
- Experimental details
- Sample (5 ?g of whole cell lysate) A: mouse brain 10% SDS PAGE GTX114439 diluted at 1:10000 The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
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- GeneTex (provider)
- Main image
- Experimental details
- Various whole cell extracts (30 ?g) were separated by 10% SDS-PAGE, and the membrane was blotted with GNAO1 antibody (GTX114439) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- Various tissue extracts (50 ?g) were separated by 10% SDS-PAGE, and the membrane was blotted with GNAO1 antibody (GTX114439) diluted at 1:10000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
Supportive validation
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- Immunofluorescence analysis of methanol-fixed HeLa, using GNAO1(GTX114439) antibody at 1:200 dilution.
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- GNAO1 antibody detects GNAO1 protein by immunofluorescent analysis.Sample: DIV9 rat E18 primary hippocampal neuron cells were fixed in 4% paraformaldehyde at RT for 15 min.Green: GNAO1 stained by GNAO1 antibody (GTX114439) diluted at 1:500.Red: beta Tubulin 3/ Tuj1, stained by beta Tubulin 3/ Tuj1 antibody [GT1338] (GTX631831) diluted at 1:500.Blue: Fluoroshield with DAPI (GTX30920).
Supportive validation
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- GeneTex (provider)
- Main image
- Experimental details
- Immunoprecipitation of GNAO1 protein from HeLa whole cell extracts using 5 £gg of GNAO1 antibody (GTX114439).Western blot analysis was performed using GNAO1 antibody (GTX114439).EasyBlot anti-Rabbit IgG (GTX221666-01) was used as a secondary reagent.
Supportive validation
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- GNAO1 antibody detects GNAO1 protein at cytoplasm on human colon carcinoma by immunohistochemical analysis. Sample: Paraffin-embedded colon carcinoma. GNAO1 antibody (GTX114439) dilution: 1:500.
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- GeneTex (provider)
- Main image
- Experimental details
- GNAO1 antibody detects GNAO1 protein at cell membrane and cytoplasm in mouse pancreas by immunohistochemical analysis. Sample: Paraffin-embedded mouse pancreas. GNAO1 antibody (GTX114439) diluted at 1:500.
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- GNAO1 antibody detects GNAO1 protein at cell membrane and cytoplasm in rat pancreas by immunohistochemical analysis. Sample: Paraffin-embedded rat pancreas. GNAO1 antibody (GTX114439) diluted at 1:500.
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- GNAO1 antibody detects GNAO1 protein by immunohistochemical analysis.Sample: Frozen-sectioned mouse cerebellum.Green: GNAO1 stained by GNAO1 antibody (GTX114439) diluted at 1:250.Red: NF-H, stained by NF-H antibody [GT114] (GTX634289) diluted at 1:500.Blue: Fluoroshield with DAPI (GTX30920).
