12635-1-AP

antibody from Invitrogen Antibodies

Targeting: GNAO1 G-ALPHA-o

Western blot (Supportive data in Antibodypedia)

Immunoprecipitation (Data presented on provider website)

Immunohistochemistry (Supportive data in Antibodypedia)

Other assay (Supportive data in Antibodypedia)

Antibody Data

Product number

12635-1-AP

Provider

Invitrogen Antibodies

Product name

GNAO1 Polyclonal Antibody

Antibody type

Polyclonal

Antigen

Other

Description

Immunogen sequence: MKIIHEDGF SGEDVKQYKP VVYSNTIQSL AAIVRAMDTL GIEYGDKERK ADAKMVCDVV SRMEDTEPFS AELLSAMMRL WGDSGIQECF NRSREYQLND SAKYYLDSLD RIGAADYQPT EQDILRTRVK TTGIVETHFT FKNLHFRLFD VGGQRSERKK WIHCFEDVTA IIFCVALSGY DQVLHEDETT NRMHESLMLF DSICNNKFFI DTSIILFLNK KDLFGEKIKK SPLTICFPEY TGPNTYEDAA AYIQAQFESK NRSPNKEIYC HMTCATDTNN IQVVFDAVTD IIIANNLRGC GLY (1-302 aa encoded by BC030027)

Reactivity

Human, Mouse, Rat

Host

Rabbit

Isotype

IgG

Vial size

150 µL

Concentration

0.22 mg/mL

Storage

-20°C

Western blot

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Human liver tissue were subjected to SDS PAGE followed by western blot with 12635-1-AP (GNAO1 antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Human brain tissue were subjected to SDS PAGE followed by western blot with 12635-1-AP (GNAO1 antibody) at dilution of 1:0 incubated at room temperature for 1.5 hours.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Rat brain tissue were subjected to SDS PAGE followed by western blot with 12635-1-AP ( GNAO1 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Y79 cells were subjected to SDS PAGE followed by western blot with 12635-1-AP (GNAO1 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Mouse testis tissue were subjected to SDS PAGE followed by western blot with 12635-1-AP (GNAO1 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

Immunohistochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemistry of paraffin-embedded human gliomas tissue slide using 12635-1-AP ( GNAO1 antibody) at dilution of 1:100 (under 10x lens) heat mediated antigen retrieved with Tris-EDTA buffer (pH 9).

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemistry of paraffin-embedded human gliomas tissue slide using 12635-1-AP ( GNAO1 antibody) at dilution of 1:100 (under 40x lens) heat mediated antigen retrieved with Tris-EDTA buffer (pH 9).

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemical analysis of paraffin-embedded human gliomas tissue slide using (Product # 12635-1-AP) (GNAO1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

Other assay

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

IP result of anti-GNAO1 (IP:12635-1-AP, 3ug; Detection:12635-1-AP 1:600) with mouse brain tissue lysate 3600ug.