RZ1172

antibody from NSJ Bioreagents

Targeting: GNAO1 G-ALPHA-o

Western blot (Supportive data in Antibodypedia)

Immunocytochemistry (Data presented on provider website)

Immunohistochemistry (Supportive data in Antibodypedia)

Antibody Data

Product number

RZ1172

Provider

NSJ Bioreagents

Product name

Zebrafish Gnao1a Antibody / Guanine nucleotide-binding protein G(o) subunit alpha

Antibody type

Polyclonal

Description

Antigen affinity purified

Reactivity

Zebrafish

Host

Rabbit

Vial size

100 ug

Concentration

Lyophilized

Storage

After reconstitution, the Zebrafish Gnao1a antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.

Western blot

Supportive validation

Submitted by

NSJ Bioreagents (provider)

Main image

Experimental details

Western blot analysis of Gnao1a protein using Zebrafish Gnao1a antibody and 1) zebrafish head, 2) whole female zebrafish, 3) whole male zebrafish and 4) zebrafish embryo tissue lysate. Predicted molecular weight ~40 kDa.

Immunohistochemistry

Supportive validation

Submitted by

NSJ Bioreagents (provider)

Main image

Experimental details

Immunofluorescent staining of FFPE zebrafish embryo tissue with Zebrafish Gnao1a antibody (green) and DAPI nuclear stain (blue). HIER: steam section in pH8 EDTA buffer for 20 min.

Supportive validation

Submitted by

NSJ Bioreagents (provider)

Main image

Experimental details

IHC staining of FFPE zebrafish kidney tissue with Zebrafish Gnao1a antibody, HRP secondary and DAB substrate. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.

Supportive validation

Submitted by

NSJ Bioreagents (provider)

Main image

Experimental details

IHC staining of FFPE zebrafish liver tissue with Zebrafish Gnao1a antibody, HRP secondary and DAB substrate. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.

Supportive validation

Submitted by

NSJ Bioreagents (provider)

Main image

Experimental details

IHC staining of FFPE zebrafish colon tissue with Zebrafish Gnao1a antibody, HRP secondary and DAB substrate. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.

Supportive validation

Submitted by

NSJ Bioreagents (provider)

Main image

Experimental details

IHC staining of FFPE zebrafish brain tissue with Zebrafish Gnao1a antibody, HRP secondary and DAB substrate. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.