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Western blotAntibody data
- Antibody Data
- Antigen structure
- References [1]
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- Validations
- Western blot [2]
- Immunohistochemistry [2]
- Other assay [1]
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- Product number
- PA5-78591 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- CHRDL1 Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Recombinant full-length protein
- Description
- Positive Control: rat brain
- Concentration
- 1.52 mg/mL
Submitted references Low expression of CHRDL1 and SPARCL1 predicts poor prognosis of lung adenocarcinoma based on comprehensive analysis and immunohistochemical validation.
Deng H, Hang Q, Shen D, Zhang Y, Chen M
Cancer cell international 2021 May 12;21(1):259
Cancer cell international 2021 May 12;21(1):259
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of CHRDL1 in rat tissue extract using 50 µg of protein. Samples were separated with 10% SDS-PAGE and incubated with CHRDL1 polyclonal antibody (Product # PA5-78591) using a dilution of 1:500.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western Blot using CHRDL1 Polyclonal Antibody (Product # PA5-78591). Untreated (–) and treated (+) HeLa whole cell extracts (30 µg) were separated by 10% SDS-PAGE, and the membrane was blotted with CHRDL1 Polyclonal Antibody (Product # PA5-78591) diluted at 1:1,000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- CHRDL1 Polyclonal Antibody detects CHRDL1 protein at cytosol on mouse hind brain by immunohistochemical analysis. Sample: Paraffin-embedded mouse hind brain. CHRDL1 Polyclonal Antibody (Product # PA5-78591) dilution: 1:500. Antigen Retrieval: EDTA based buffer, pH 8.0, 15 min.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- CHRDL1 Polyclonal Antibody detects CHRDL1 protein at cytosol on mouse kidney by immunohistochemical analysis. Sample: Paraffin-embedded mouse kidney. CHRDL1 Polyclonal Antibody (Product # PA5-78591) dilution: 1:500. Antigen Retrieval: EDTA based buffer, pH 8.0, 15 min.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Fig. 10 The distribution and expression of CHRDL1 and SPARCL1 proteins in twenty pairs of LUAD and normal tissues. Representative pictures of immunohistochemistry of CHRDL1 and SPARCL1 proteins are shown ( a, b ). The score of immunohistochemistry of CHRDL1 and SPARCL1 proteins are displayed ( c, d ). ***P = 0.001; ****P < 0.001
