Explore
Validate
Learn
Western blotAntibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [2]
- Immunoprecipitation [1]
- Immunohistochemistry [2]
Submit
Validation data
Reference
Comment
Report error
- Product number
- NB100-59840 - Provider product page
- Provider
- Novus Biologicals
- Proper citation
- Novus Cat#NB100-59840, RRID:AB_2233082
- Product name
- Rabbit Polyclonal HMGN3/TRIP7 Antibody
- Antibody type
- Polyclonal
- Description
- Immunogen affinity purified.
- Reactivity
- Human
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 0.1 ml
- Concentration
- 0.2 mg/ml
- Storage
- Store at 4C. Do not freeze.
No comments: Submit comment
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: HMGN3/TRIP7 Antibody [NB100-59840] - Detection of Human HMGN3 on HeLa whole cell lysate using NB100-59840. HMGN3/TRIP7 was also immunoprecipitated by rabbit anti-HMGN3 antibody NB100-59839.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: HMGN3/TRIP7 Antibody [NB100-59840] - Detection of human HMGN3 by western blot. Samples: Whole cell lysate from HeLa (15 and 50 ug), HEK293T (50ug), and Jurkat (50ug) cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-HMGN3 antibody NB100-59840 used for WB at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunoprecipitation: HMGN3/TRIP7 Antibody [NB100-59840] - Detection of human HMGN3 by western blot of immunoprecipitates. Samples: Whole cell lysate (0.5 or 1.0 mg per IP reaction; 20% of IP loaded) from HeLa cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-HMGN3 antibody NB100-59840 (lot 2) used for IP at 6 ug per reaction. HMGN3 was also immunoprecipitated with a previous lot of this antibody (lot 1) and rabbit anti-HMGN3 antibody NB100-59839 (lots 1 and 2). For blotting immunoprecipitated HGMN3, NB100-59840 was used at 1 ug/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry-Paraffin: HMGN3/TRIP7 Antibody [NB100-59840] - Human prostate carcinoma. Antibody used at a dilution of 1:1,000.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry: HMGN3/TRIP7 Antibody [NB100-59840] - Detection of human HMGN3 by immunohistochemistry. Sample: FFPE section of human lung carcinoma. Antibody: Affinity purified rabbit anti-HMGN3 (NB100-59840). Detection: DAB
