Antibody data
- Antibody Data
- Antigen structure
- References [6]
- Comments [0]
- Validations
- Flow cytometry [2]
- Other assay [2]
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Validation data
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- Product number
- 12-0106-42 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- CD10 Monoclonal Antibody (eBioCB-CALLA (CB-CALLA)), PE, eBioscience™
- Antibody type
- Monoclonal
- Antigen
- Other
- Description
- Description: The eBioCB-CALLA monoclonal antibody recognizes human CD10 (CALLA, NEP, enkephalinase, Neprilysin), which is a 100 kDa, type II cell surface glycoprotein originally identified for its expression on most acute lymphoblastic leukemias (ALL). Subsequently, CD10 was shown to be the same molecule as the neutral endopeptidase (NEP), or KII-NA. CD10 is a Zn2+-dependent metallo-peptidase with endothelin, glucagon, gastrin, neurotensin and bradykinin included among its substrates. CD10 is involved in the regulation of chemotactic and inflammatory processes involving neutrophils. In B cells, CD10 regulates stromal cell-dependent B lymphopoiesis and expression has also been reported on mature B cells in germinal centres. In addition to the hematopoietic compartment, other major sites of CD10 expression are the brush border of enterocytes and renal tubules and glomeruli. There is partial blocking of the eBioCB-CALLA and MEM-78 monoclonal antibodies indicating that they recognize similar epitopes. Applications Reported: This eBioCB-CALLA (CB-CALLA) antibody has been reported for use in flow cytometric analysis. Applications Tested: This eBioCB-CALLA (CB-CALLA) antibody has been pre-titrated and tested by flow cytometric analysis of normal human peripheral blood cells. This can be used at 5 µL (0.06 µg) per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. Excitation: 488-561 nm; Emission: 578 nm; Laser: Blue Laser, Green Laser, Yellow-Green Laser. Filtration: 0.2 µm post-manufacturing filtered.
- Reactivity
- Human
- Host
- Mouse
- Conjugate
- Yellow dye
- Isotype
- IgG
- Antibody clone number
- eBioCB-CALLA (CB-CALLA)
- Vial size
- 100 Tests
- Concentration
- 5 µL/Test
- Storage
- 4° C, store in dark, DO NOT FREEZE!
Submitted references Endometriosis-Associated Mesenchymal Stem Cells Support Ovarian Clear Cell Carcinoma through Iron Regulation.
Heterogeneous disease-propagating stem cells in juvenile myelomonocytic leukemia.
Elevated Calprotectin and Abnormal Myeloid Cell Subsets Discriminate Severe from Mild COVID-19.
Analysis of the Transcriptome: Regulation of Cancer Stemness in Breast Ductal Carcinoma In Situ by Vitamin D Compounds.
Antithymocyte Globulin at Clinically Relevant Concentrations Kills Leukemic Blasts.
Stem-like epithelial cells are concentrated in the distal end of the fallopian tube: a site for injury and serous cancer initiation.
Atiya HI, Frisbie L, Goldfeld E, Orellana T, Donnellan N, Modugno F, Calderon M, Watkins S, Zhang R, Elishaev E, Soong TR, Vlad A, Coffman L
Cancer research 2022 Dec 16;82(24):4680-4693
Cancer research 2022 Dec 16;82(24):4680-4693
Heterogeneous disease-propagating stem cells in juvenile myelomonocytic leukemia.
Louka E, Povinelli B, Rodriguez-Meira A, Buck G, Wen WX, Wang G, Sousos N, Ashley N, Hamblin A, Booth CAG, Roy A, Elliott N, Iskander D, de la Fuente J, Fordham N, O'Byrne S, Inglott S, Norfo R, Salio M, Thongjuea S, Rao A, Roberts I, Mead AJ
The Journal of experimental medicine 2021 Feb 1;218(2)
The Journal of experimental medicine 2021 Feb 1;218(2)
Elevated Calprotectin and Abnormal Myeloid Cell Subsets Discriminate Severe from Mild COVID-19.
Silvin A, Chapuis N, Dunsmore G, Goubet AG, Dubuisson A, Derosa L, Almire C, Hénon C, Kosmider O, Droin N, Rameau P, Catelain C, Alfaro A, Dussiau C, Friedrich C, Sourdeau E, Marin N, Szwebel TA, Cantin D, Mouthon L, Borderie D, Deloger M, Bredel D, Mouraud S, Drubay D, Andrieu M, Lhonneur AS, Saada V, Stoclin A, Willekens C, Pommeret F, Griscelli F, Ng LG, Zhang Z, Bost P, Amit I, Barlesi F, Marabelle A, Pène F, Gachot B, André F, Zitvogel L, Ginhoux F, Fontenay M, Solary E
Cell 2020 Sep 17;182(6):1401-1418.e18
Cell 2020 Sep 17;182(6):1401-1418.e18
Analysis of the Transcriptome: Regulation of Cancer Stemness in Breast Ductal Carcinoma In Situ by Vitamin D Compounds.
Shan NL, Minden A, Furmanski P, Bak MJ, Cai L, Wernyj R, Sargsyan D, Cheng D, Wu R, Kuo HD, Li SN, Fang M, Maehr H, Kong AN, Suh N
Cancer prevention research (Philadelphia, Pa.) 2020 Aug;13(8):673-686
Cancer prevention research (Philadelphia, Pa.) 2020 Aug;13(8):673-686
Antithymocyte Globulin at Clinically Relevant Concentrations Kills Leukemic Blasts.
Dabas R, Lee R, Servito MT, Dharmani-Khan P, Modi M, van Slyke T, Luider J, Durand C, Larratt L, Brandwein J, Morris D, Daly A, Khan FM, Storek J
Biology of blood and marrow transplantation : journal of the American Society for Blood and Marrow Transplantation 2016 May;22(5):815-24
Biology of blood and marrow transplantation : journal of the American Society for Blood and Marrow Transplantation 2016 May;22(5):815-24
Stem-like epithelial cells are concentrated in the distal end of the fallopian tube: a site for injury and serous cancer initiation.
Paik DY, Janzen DM, Schafenacker AM, Velasco VS, Shung MS, Cheng D, Huang J, Witte ON, Memarzadeh S
Stem cells (Dayton, Ohio) 2012 Nov;30(11):2487-97
Stem cells (Dayton, Ohio) 2012 Nov;30(11):2487-97
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Knockout of CD10 was achieved by CRISPR-Cas9 genome editing using LentiArray™ Lentiviral sgRNA (Product # A32042, Assay ID CRISPR1056041_LV) and LentiArray Cas9 Lentivirus (Product # A32064). Flow cytometry analysis of CD10 was performed by staining LNCaP CD10 Knock out cells with 0.06 µg Mouse IgG2b kappa Isotype Control (eBMG2b), PE, eBioscience™ (Product # 12-4732-81, yellow histogram) or 0.06 µg CD10 Monoclonal Antibody (eBioCB-CALLA (CB-CALLA)), PE, eBioscience™ (Product # 12-0106-42, blue histogram). LNCaP Cas9 control cells were also stained with0.06 µg CD10 Monoclonal Antibody (eBioCB-CALLA (CB-CALLA)), PE, eBioscience™ (Product # 12-0106-42, pink histogram). Lossof signal was observed in the CD10 KOcells stained with CD10 antibody clone eBioCB-CALLA (CB-CALLA) but not in the control Cas9cells. Viable cells were used for analysis, as determined by Fixable Viability DyeeFluor™780 (Product # 65-0865-18).
- Conjugate
- Yellow dye
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Staining of normal human peripheral blood cells with Anti-Human CD10 PE. Cells in the lymphocyte (blue histogram) and granulocyte (purple histogram) gates were used for analysis.
- Conjugate
- Yellow dye
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- NULL
- Conjugate
- Yellow dye
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Figure 4 Single-Cell Analysis of Neutrophils by scRNA-Seq, Spectral Flow Cytometry, and Mass Cytometry (A) UMAP profile of neutrophils in the 9 samples analyzed as described in Figure 2 A. (B) UMAP profile of neutrophils within the 3 controls and the mild and the two severe cases with the cluster gates overlaid. (C) Violin plots of expression of the indicated genes in two statistically defined neutrophil clusters. (D) Heatmap of DEGs in total neutrophils generated as described in Figure 3 B. (E and F) Spectral flow analysis of neutrophil subsets in pooled controls and each individual patient sample at day 0 and day 10, based on CD10 and CD101 expression (E) and CXCR4 and CD11b expression among CD10 Low CD101 - neutrophils (F) in the indicated samples (pooled controls). (G and H) Mass cytometry analysis of neutrophil subsets in 4 patients within each group (pooled data) as in Figures 3 F-3I, based on CD10 and CD101 expression (G) and the fraction of CD10 Low CD101 - neutrophils among total neutrophils in each sample within the 3 groups (H). Kruskal-Wallis test, * p < 0.05.
- Conjugate
- Yellow dye