Antibody data
- Antibody Data
- Antigen structure
- References [2]
- Comments [0]
- Validations
- Flow cytometry [1]
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Validation data
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- Product number
- 13-0106-80 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- Anti-CD10 Monoclonal Antibody (eBioCB-CALLA (CB-CALLA)), Biotin, eBioscience™
- Antibody type
- Monoclonal
- Antigen
- Other
- Description
- Description: The eBioCB-CALLA monoclonal antibody recognizes human CD10 (CALLA, NEP, enkephalinase, Neprilysin), which is a 100 kDa, type II cell surface glycoprotein originally identified for its expression on most acute lymphoblastic leukemias (ALL). Subsequently, CD10 was shown to be the same molecule as the neutral endopeptidase (NEP), or KII-NA. CD10 is a Zn2+-dependent metallo-peptidase with endothelin, glucagon, gastrin, neurotensin and bradykinin included among its substrates. CD10 is involved in the regulation of chemotactic and inflammatory processes involving neutrophils. In B cells, CD10 regulates stromal cell-dependent B lymphopoiesis and expression has also been reported on mature B cells in germinal centres. In addition to the hematopoietic compartment, other major sites of CD10 expression are the brush border of enterocytes and renal tubules and glomeruli. There is partial blocking of the eBioCB-CALLA and MEM-78 monoclonal antibodies indicating that they recognize similar epitopes. Applications Reported: This eBioCB-CALLA (CB-CALLA) antibody has been reported for use in flow cytometric analysis. Applications Tested: This eBioCB-CALLA (CB-CALLA) antibody has been tested by flow cytometric analysis of normal normal human peripheral blood cells. This can be used at less than or equal to 0.5 µg per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.carefully titrated for optimal performance in the assay of interest. Filtration: 0.2 µm post-manufacturing filtered.
- Reactivity
- Human
- Host
- Mouse
- Conjugate
- Biotin
- Isotype
- IgG
- Antibody clone number
- eBioCB-CALLA (CB-CALLA)
- Vial size
- 25 µg
- Concentration
- 0.5 mg/mL
- Storage
- 4° C, store in dark, DO NOT FREEZE!
Submitted references Simvastatin inhibits the apoptosis of hippocampal cells in a mouse model of Alzheimer's disease.
Targeting CD22 with the monoclonal antibody epratuzumab modulates human B-cell maturation and cytokine production in response to Toll-like receptor 7 (TLR7) and B-cell receptor (BCR) signaling.
Hu X, Song C, Fang M, Li C
Experimental and therapeutic medicine 2018 Feb;15(2):1795-1802
Experimental and therapeutic medicine 2018 Feb;15(2):1795-1802
Targeting CD22 with the monoclonal antibody epratuzumab modulates human B-cell maturation and cytokine production in response to Toll-like receptor 7 (TLR7) and B-cell receptor (BCR) signaling.
Giltiay NV, Shu GL, Shock A, Clark EA
Arthritis research & therapy 2017 May 15;19(1):91
Arthritis research & therapy 2017 May 15;19(1):91
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Staining of normal human peripheral blood cells with 0.25 µg of Mouse IgG2b K Isotype Control Biotin Product # 13-4732-85) (open histogram) or 0.25 µg of Anti-Human CD10 Biotin (filled histogram) followed by Streptavidin PE (Product # 12-4317-87). Cells in the large scatter population were used for analysis.
- Conjugate
- Biotin