13-0108-82

antibody from Invitrogen Antibodies
Targeting: MME CALLA, CD10, NEP
Provider product page for 13-0108-82
Immunohistochemistry
Flow cytometry

Antibody data

Product number
13-0108-82 - Provider product page
Provider
Invitrogen Antibodies
Product name
CD10 Monoclonal Antibody (SN5c), Biotin, eBioscience™
Antibody type
Monoclonal
Antigen
Other
Description
Description: The eBioSN5c monoclonal antibody recognizes human CD10 (CALLA, NEP, enkephalinase, Neprilysin), which is a 100 kDa, type II cell surface glycoprotein originally identified for its expression on most acute lymphoblastic leukemias (ALLs). Subsequently, CD10 was shown to be the same molecule as neutral endopeptidase (NEP), or KII-NA. CD10 is a Zn^2+-dependent metallopeptidase acting on substrates such as endothelin, glucagon, gastrin, neurotensin and bradykinin. CD10 is involved in the regulation of chemotactic and inflammatory processes involving neutrophils. In B cells, CD10 regulates stromal cell-dependent B lymphopoiesis and its expression has been reported on mature germinal center B cells. CD10 expression is also found on normal donor granulocytes and bone marrow stromal cells. Most likely, CB-CALLA and eBioSN5c see different epitopes due to their ability to co-stain. Applications Reported: This eBioSN5c (SN5c L4-1A1) antibody has been reported for use in flow cytometric analysis, and immunohistology staining of frozen tissue sections. Applications Tested: This eBioSN5c (SN5c L4-1A1) antibody has been tested by flow cytometric analysis. This can be used at less than or equal to 0.25 µg per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest. Filtration: 0.2 µm post-manufacturing filtered.
Reactivity
Human
Host
Mouse
Conjugate
Biotin
Isotype
IgG
Antibody clone number
SN5c
Vial size
100 µg
Concentration
0.5 mg/mL
Storage
4° C, store in dark, DO NOT FREEZE!
MME protein structure - 13-0108-82 shown in red.