Antibody data
- Antibody Data
- Antigen structure
- References [5]
- Comments [0]
- Validations
- Flow cytometry [1]
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- Product number
- 46-0108-42 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- CD10 Monoclonal Antibody (SN5c), PerCP-eFluor™ 710, eBioscience™
- Antibody type
- Monoclonal
- Antigen
- Other
- Description
- Description: The eBioSN5c monoclonal antibody recognizes human CD10 (CALLA, NEP, enkephalinase, Neprilysin), which is a 100 kDa, type II cell surface glycoprotein originally identified for its expression on most acute lymphoblastic leukemias (ALLs). Subsequently, CD10 was shown to be the same molecule as neutral endopeptidase (NEP), or KII-NA. CD10 is a Zn^2+-dependent metallopeptidase acting on substrates such as endothelin, glucagon, gastrin, neurotensin and bradykinin. CD10 is involved in the regulation of chemotactic and inflammatory processes involving neutrophils. In B cells, CD10 regulates stromal cell-dependent B lymphopoiesis and its expression has been reported on mature germinal center B cells. CD10 expression is also found on normal donor granulocytes and bone marrow stromal cells. Most likely, CB-CALLA and eBioSN5c see different epitopes due to their ability to co-stain. Applications Reported: This eBioSN5c (SN5c L4-1A1) antibody has been reported for use in flow cytometric analysis. Applications Tested: This SN5c antibody has been pre-titrated and tested by flow cytometric analysis of normal human peripheral blood cells. This can be used at 5 µL (0.25 µg) per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. PerCP-eFluor® 710 emits at 710 nm and is excited with the blue laser (488 nm); it can be used in place of PerCP-Cyanine5.5. We recommend using a 710/50 bandpass filter, however, the 695/40 bandpass filter is an acceptable alternative. Please make sure that your instrument is capable of detecting this fluorochrome. Fixation: Samples can be stored in IC Fixation Buffer (Product # 00-822-49) (100 µL cell sample + 100 µL IC Fixation Buffer) or 1-step Fix/Lyse Solution (Product # 00-5333-54) for up to 3 days in the dark at 4°C with minimal impact on brightness and FRET efficiency/compensation. Some generalizations regarding fluorophore performance after fixation can be made, but clone specific performance should be determined empirically. Excitation: 488 nm; Emission: 710 nm; Laser: Blue Laser. Filtration: 0.2 µm post-manufacturing filtered.
- Reactivity
- Human
- Host
- Mouse
- Isotype
- IgG
- Antibody clone number
- SN5c
- Vial size
- 100 Tests
- Concentration
- 5 µL/Test
- Storage
- 4° C, store in dark, DO NOT FREEZE!
Submitted references Proline‑rich polypeptide‑1 decreases cancer stem cell population by targeting BAFF chromatin‑remodeling complexes in human chondrosarcoma JJ012 cells.
More than one antibody of individual B cells revealed by single-cell immune profiling.
Dendritic cell profile induced by Schistosoma mansoni antigen in cutaneous leishmaniasis patients.
Alterations in peripheral blood B cell subsets and dynamics of B cell responses during human schistosomiasis.
Unique epitopes of common acute lymphoblastic leukemia antigen detected by new monoclonal antibodies.
Moran A, Hoyt A, Sedani A, Granger C, Saigh S, Blonska M, Zhao-Ju L, Conway SA, Pretell J, Brown J, Galoian K
Oncology reports 2020 Jul;44(1):393-403
Oncology reports 2020 Jul;44(1):393-403
More than one antibody of individual B cells revealed by single-cell immune profiling.
Shi Z, Zhang Q, Yan H, Yang Y, Wang P, Zhang Y, Deng Z, Yu M, Zhou W, Wang Q, Yang X, Mo X, Zhang C, Huang J, Dai H, Sun B, Zhao Y, Zhang L, Yang YG, Qiu X
Cell discovery 2019;5:64
Cell discovery 2019;5:64
Dendritic cell profile induced by Schistosoma mansoni antigen in cutaneous leishmaniasis patients.
Lopes DM, Fernandes JS, Cardoso TM, Bafica AM, Oliveira SC, Carvalho EM, Araujo MI, Cardoso LS
BioMed research international 2014;2014:743069
BioMed research international 2014;2014:743069
Alterations in peripheral blood B cell subsets and dynamics of B cell responses during human schistosomiasis.
Labuda LA, Ateba-Ngoa U, Feugap EN, Heeringa JJ, van der Vlugt LE, Pires RB, Mewono L, Kremsner PG, van Zelm MC, Adegnika AA, Yazdanbakhsh M, Smits HH
PLoS neglected tropical diseases 2013;7(3):e2094
PLoS neglected tropical diseases 2013;7(3):e2094
Unique epitopes of common acute lymphoblastic leukemia antigen detected by new monoclonal antibodies.
Matsuzaki H, Haruta Y, Fukukawa T, Barcos MP, Seon BK
Cancer research 1987 Apr 15;47(8):2160-6
Cancer research 1987 Apr 15;47(8):2160-6
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Supportive validation
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- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Staining of normal human peripheral blood cells with Mouse IgG1 K Isotype Control PerCP-eFluor® 710 (Product # 46-4714-82) (blue histogram) or Anti-Human CD10 PerCP-eFluor® 710 (purple histogram). Cells in the lymphocyte (left) or granulocyte (right) gate were used for analysis.