Antibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [1]
- Immunohistochemistry [1]
- Flow cytometry [1]
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Validation data
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- Product number
- 10-7560-25 - Provider product page
- Provider
- ABEOMICS Inc.
- Product name
- Anti-CD10 Antibody
- Antibody type
- Monoclonal
- Description
- CD10, also known as membrane metalloendopeptidase, neutral endopeptidase, neprilysin and common acute lymphoblastic leukaemia antigen (CALLA), is a zinc-dependent metalloendoprotease that cleaves signaling peptides. It is widely expressed in various normal tissues and in epithelial, stromal or both components of various malignancies. CD10 is a marker for intestinal epithelial brush border. It is also present in normal bile ducts and gallbladder epithelia but is absent in cholangiocarcinomas. It is con-sidered as the prototype of this family of membrane-bound, zinc-dependent endopeptidases, which regulate the physiological action of various peptides by lowering their extracellular concentration available for receptor binding. CD10 is also known to be an amyloid b-peptide (Ab) degrading enzyme. Polymorphisms in the CD10 gene increase the risk for Alzheimers disease. Outside the nervous system, it is present on the surface of neutrophils and regulates their activation by degradation of in?ammatory peptides.
- Reactivity
- Human
- Host
- Mouse
- Conjugate
- Unconjugated
- Antigen sequence
A partial length recombinant CD10 p
rotein (amino acids 282-496) was us
ed as the immunogen for this antibo
dy.- Isotype
- IgG
- Antibody clone number
- ABM4A52
- Vial size
- 100 µg
- Concentration
- 0.5 mg/ml
- Storage
- Store the antibody at 4°C, stable for 6 months. For long-term storage, store at -20°C. Avoid repeat freez thawing
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Supportive validation
- Submitted by
- ABEOMICS Inc. (provider)
- Main image
- Experimental details
- Western blot analysis of CD10. Anti-CD10 antibody (Clone: ABM4A52) was tested at 0.5 µg/ml on Jurkat and Ramos lysates.
- Protocol
- Protocol
Supportive validation
- Submitted by
- ABEOMICS Inc. (provider)
- Main image
- Experimental details
- Immunohistochemical analysis of CD10 in human Renal CellCarcinoma tissue using CD10 antibody (Clone: ABM4A52) at 5 µg/ml.
- Protocol
- Protocol
Supportive validation
- Submitted by
- ABEOMICS Inc. (provider)
- Main image
- Experimental details
- Cell surface flow analysis of CD10 (10-7560) in PBMC using 0.5 µg/10^6 cells of CD10 antibody (Clone: ABM4A52). Green represents isotype control (ABEOMICS); red represents anti-CD10 antibody. Goat anti-Mouse PE conjugate was used as secondary.
- Protocol
- Protocol