MA1-19627

antibody from Invitrogen Antibodies

Targeting: MME CALLA, CD10, NEP

Antibody data

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Validation data

MME protein structure - MA1-19627 shown in red.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Flow cytometry analysis of CD10 using a monoclonal antibody (Product # MA1-19627).
Conjugate: Yellow dye

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Flow cytometry surface staining pattern of human peripheral whole blood stained using anti-human CD10 (MEM-78) PE Monoclonal antibody (Product # MA1-19627) using a dilution of 20 µL reagent/100 µL of peripheral whole blood.
Conjugate: Yellow dye

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Knockout of CD10 was achieved by CRISPR-Cas9 genome editing using LentiArray™ Lentiviral sgRNA (Product # A32042, Assay ID CRISPR_LV, Assay ID CRISPR1056041_LV) and LentiArray Cas9 Lentivirus (Product # A32064). Flow cytometry analysis of CD10 was performed by staining LNCaP CD10 Knock out cells (blue histogram) or LNCaP Cas9 control cells (pink histogram) with 20 µl CD10 Monoclonal Antibody (MEM-78), PE (Product # MA1-19627). Lossof signal was observed in the CD10 KOcells stained with CD10 antibody clone MEM-78 but not in the control Cas9cells. Viable cells were used for analysis, as determined by Fixable Viability DyeeFluor™780 (Product # 65-0865-18).
Conjugate: Yellow dye

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Separation of human neutrophil granulocytes (red-filled) from CD10 negative lymphocytes (black-dashed) in flow cytometry analysis (surface staining) of human peripheral whole blood stained using anti-human CD10 (MEM-78) PE Monoclonal antibody (Product # MA1-19627) using a dilution of 20 µL reagent/100 µL of peripheral whole blood.
Conjugate: Yellow dye