Antibody data
- Antibody Data
- Antigen structure
- References [1]
- Comments [0]
- Validations
- Flow cytometry [4]
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Validation data
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- Product number
- MA1-19627 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- CD10 Monoclonal Antibody (MEM-78), PE
- Antibody type
- Monoclonal
- Antigen
- Other
- Description
- This antibody reacts with an extracellular epitope CD10 antigen (CALLA - Common acute lymphatic leukemia antigen), a 100 kDa type II integral membrane protein.
- Reactivity
- Human
- Host
- Mouse
- Conjugate
- Yellow dye
- Isotype
- IgG
- Antibody clone number
- MEM-78
- Vial size
- 100 Tests
- Storage
- 4° C, store in dark, DO NOT FREEZE!
Submitted references Staphylococcus aureus Staphopain A inhibits CXCR2-dependent neutrophil activation and chemotaxis.
Laarman AJ, Mijnheer G, Mootz JM, van Rooijen WJ, Ruyken M, Malone CL, Heezius EC, Ward R, Milligan G, van Strijp JA, de Haas CJ, Horswill AR, van Kessel KP, Rooijakkers SH
The EMBO journal 2012 Aug 29;31(17):3607-19
The EMBO journal 2012 Aug 29;31(17):3607-19
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Flow cytometry analysis of CD10 using a monoclonal antibody (Product # MA1-19627).
- Conjugate
- Yellow dye
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Flow cytometry surface staining pattern of human peripheral whole blood stained using anti-human CD10 (MEM-78) PE Monoclonal antibody (Product # MA1-19627) using a dilution of 20 µL reagent/100 µL of peripheral whole blood.
- Conjugate
- Yellow dye
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Knockout of CD10 was achieved by CRISPR-Cas9 genome editing using LentiArray™ Lentiviral sgRNA (Product # A32042, Assay ID CRISPR_LV, Assay ID CRISPR1056041_LV) and LentiArray Cas9 Lentivirus (Product # A32064). Flow cytometry analysis of CD10 was performed by staining LNCaP CD10 Knock out cells (blue histogram) or LNCaP Cas9 control cells (pink histogram) with 20 µl CD10 Monoclonal Antibody (MEM-78), PE (Product # MA1-19627). Lossof signal was observed in the CD10 KOcells stained with CD10 antibody clone MEM-78 but not in the control Cas9cells. Viable cells were used for analysis, as determined by Fixable Viability DyeeFluor™780 (Product # 65-0865-18).
- Conjugate
- Yellow dye
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Separation of human neutrophil granulocytes (red-filled) from CD10 negative lymphocytes (black-dashed) in flow cytometry analysis (surface staining) of human peripheral whole blood stained using anti-human CD10 (MEM-78) PE Monoclonal antibody (Product # MA1-19627) using a dilution of 20 µL reagent/100 µL of peripheral whole blood.
- Conjugate
- Yellow dye