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ImmunohistochemistryAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Immunohistochemistry [1]
- Flow cytometry [1]
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- Product number
- NBP2-34339-0.1mg - Provider product page
- Provider
- Novus Biologicals
- Product name
- Mouse Monoclonal Melanoma Marker (MART-1 + Tyrosinase + gp100) Antibody
- Antibody type
- Monoclonal
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Supportive validation
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- Novus Biologicals (provider)
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- Experimental details
- Immunohistochemistry-Paraffin: Melanoma Marker (MART-1 + Tyrosinase + gp100) Antibody (A103 + T311 + HMB45) [NBP2-34339] - Formalin-fixed, paraffin-embedded human melanoma stained with Melanoma Marker MAb (A103 + T311 + HMB45).
Supportive validation
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- Novus Biologicals (provider)
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- Experimental details
- Flow (Intracellular): Melanoma Marker (MART-1 + Tyrosinase + gp100) Antibody (A103 + T311 + HMB45) [NBP2-34339] - An intracellular stain was performed on SK-MEL-28 cells with Melanoma Marker (MART-1 + Tyrosinase + gp100) Antibody NBP2-34547R (blue) and a matched isotype control (orange). Cells were fixed with 4% PFA and then permeabilized with 0.1% saponin. Cells were incubated in an antibody dilution of 5 ug/mL for 30 minutes at room temperature. Both antibodies were conjugated to DyLight 550. Image using the Azide and BSA Free format of this antibody.
