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Western blotAntibody data
- Antibody Data
- Antigen structure
- References [18]
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- Validations
- Western blot [1]
- Immunohistochemistry [2]
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- Product number
- MA5-14168 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- Melan-A Monoclonal Antibody (A103)
- Antibody type
- Monoclonal
- Antigen
- Recombinant full-length protein
- Description
- MA5-14168 targets MART-1 in IHC (P) and WB applications and shows reactivity with Human samples.
- Antibody clone number
- A103
- Concentration
- 0.2 mg/mL
Submitted references Xp11.2 translocation renal cell carcinoma with NONO-TFE3 gene fusion: morphology, prognosis, and potential pitfall in detecting TFE3 gene rearrangement.
Primary sclerosing epithelioid fibrosarcoma of kidney with variant histomorphologic features: report of 2 cases and review of the literature.
Targeting histone deacetylase 6 mediates a dual anti-melanoma effect: Enhanced antitumor immunity and impaired cell proliferation.
Loss of BRM expression is a frequently observed event in poorly differentiated clear cell renal cell carcinoma.
Renal cell carcinomas with t(6;11)(p21;q12) presenting with tubulocystic renal cell carcinoma-like features.
Cathepsin K expression in a wide spectrum of perivascular epithelioid cell neoplasms (PEComas): a clinicopathological study emphasizing extrarenal PEComas.
Renal cell carcinomas with t(6;11)(p21;q12): A clinicopathologic study emphasizing unusual morphology, novel alpha-TFEB gene fusion point, immunobiomarkers, and ultrastructural features, as well as detection of the gene fusion by fluorescence in situ hybridization.
Expression of E-cadherin in angiomyolipoma.
Sporadic haemangioblastoma of the kidney with rhabdoid features and focal CD10 expression: report of a case and literature review.
Microscopic uterine lymphangioleiomyomatosis perivascular epithelioid cell neoplasm: a case report with the earliest manifestation of this enigmatic neoplasm.
Polypoid PEComa in the rectum of a 15-year-old girl: case report and review of PEComa in the gastrointestinal tract.
Melanocytes expressing MC1R polymorphisms associated with red hair color have altered MSH-ligand activated pigmentary responses in coculture with keratinocytes.
Diagnostic challenges of metastatic spindle cell melanoma on fine-needle aspiration specimens.
Acral lentiginous melanoma: an immunohistochemical study of 20 cases.
Acral lentiginous melanoma: an immunohistochemical study of 20 cases.
Hyperpigmentation during interferon-alpha therapy for chronic hepatitis C virus infection.
Hyperpigmentation during interferon-alpha therapy for chronic hepatitis C virus infection.
Comparison of five antibodies as markers in the diagnosis of melanoma in cytologic preparations.
Xia QY, Wang Z, Chen N, Gan HL, Teng XD, Shi SS, Wang X, Wei X, Ye SB, Li R, Ma HH, Lu ZF, Zhou XJ, Rao Q
Modern pathology : an official journal of the United States and Canadian Academy of Pathology, Inc 2017 Mar;30(3):416-426
Modern pathology : an official journal of the United States and Canadian Academy of Pathology, Inc 2017 Mar;30(3):416-426
Primary sclerosing epithelioid fibrosarcoma of kidney with variant histomorphologic features: report of 2 cases and review of the literature.
Ertoy Baydar D, Kosemehmetoglu K, Aydin O, Bridge JA, Buyukeren B, Aki FT
Diagnostic pathology 2015 Oct 9;10:186
Diagnostic pathology 2015 Oct 9;10:186
Targeting histone deacetylase 6 mediates a dual anti-melanoma effect: Enhanced antitumor immunity and impaired cell proliferation.
Woan KV, Lienlaf M, Perez-Villaroel P, Lee C, Cheng F, Knox T, Woods DM, Barrios K, Powers J, Sahakian E, Wang HW, Canales J, Marante D, Smalley KSM, Bergman J, Seto E, Kozikowski A, Pinilla-Ibarz J, Sarnaik A, Celis E, Weber J, Sotomayor EM, Villagra A
Molecular oncology 2015 Aug;9(7):1447-1457
Molecular oncology 2015 Aug;9(7):1447-1457
Loss of BRM expression is a frequently observed event in poorly differentiated clear cell renal cell carcinoma.
Xia QY, Rao Q, Cheng L, Shen Q, Shi SS, Li L, Liu B, Zhang J, Wang YF, Shi QL, Wang JD, Ma HH, Lu ZF, Yu B, Zhang RS, Zhou XJ
Histopathology 2014 May;64(6):847-62
Histopathology 2014 May;64(6):847-62
Renal cell carcinomas with t(6;11)(p21;q12) presenting with tubulocystic renal cell carcinoma-like features.
Rao Q, Zhang XM, Tu P, Xia QY, Shen Q, Zhou XJ, Shi QL
International journal of clinical and experimental pathology 2013;6(7):1452-7
International journal of clinical and experimental pathology 2013;6(7):1452-7
Cathepsin K expression in a wide spectrum of perivascular epithelioid cell neoplasms (PEComas): a clinicopathological study emphasizing extrarenal PEComas.
Rao Q, Cheng L, Xia QY, Liu B, Li L, Shi QL, Shi SS, Yu B, Zhang RS, Ma HH, Lu ZF, Tu P, Zhou XJ
Histopathology 2013 Mar;62(4):642-50
Histopathology 2013 Mar;62(4):642-50
Renal cell carcinomas with t(6;11)(p21;q12): A clinicopathologic study emphasizing unusual morphology, novel alpha-TFEB gene fusion point, immunobiomarkers, and ultrastructural features, as well as detection of the gene fusion by fluorescence in situ hybridization.
Rao Q, Liu B, Cheng L, Zhu Y, Shi QL, Wu B, Jiang SJ, Wang Y, Wang X, Yu B, Zhang RS, Ma HH, Lu ZF, Tu P, Wang JD, Zhou XJ
The American journal of surgical pathology 2012 Sep;36(9):1327-38
The American journal of surgical pathology 2012 Sep;36(9):1327-38
Expression of E-cadherin in angiomyolipoma.
Wang Z, Gong Q, Fan Q
Human pathology 2012 Dec;43(12):2348-53
Human pathology 2012 Dec;43(12):2348-53
Sporadic haemangioblastoma of the kidney with rhabdoid features and focal CD10 expression: report of a case and literature review.
Yin WH, Li J, Chan JK
Diagnostic pathology 2012 Apr 12;7:39
Diagnostic pathology 2012 Apr 12;7:39
Microscopic uterine lymphangioleiomyomatosis perivascular epithelioid cell neoplasm: a case report with the earliest manifestation of this enigmatic neoplasm.
Clay MR, Gibson P, Lowell J, Cooper K
International journal of gynecological pathology : official journal of the International Society of Gynecological Pathologists 2011 Jan;30(1):71-5
International journal of gynecological pathology : official journal of the International Society of Gynecological Pathologists 2011 Jan;30(1):71-5
Polypoid PEComa in the rectum of a 15-year-old girl: case report and review of PEComa in the gastrointestinal tract.
Ryan P, Nguyen VH, Gholoum S, Carpineta L, Abish S, Ahmed NN, Laberge JM, Riddell RH
The American journal of surgical pathology 2009 Mar;33(3):475-82
The American journal of surgical pathology 2009 Mar;33(3):475-82
Melanocytes expressing MC1R polymorphisms associated with red hair color have altered MSH-ligand activated pigmentary responses in coculture with keratinocytes.
Roberts DW, Newton RA, Leonard JH, Sturm RA
Journal of cellular physiology 2008 May;215(2):344-55
Journal of cellular physiology 2008 May;215(2):344-55
Diagnostic challenges of metastatic spindle cell melanoma on fine-needle aspiration specimens.
Piao Y, Guo M, Gong Y
Cancer 2008 Apr 25;114(2):94-101
Cancer 2008 Apr 25;114(2):94-101
Acral lentiginous melanoma: an immunohistochemical study of 20 cases.
Kim YC, Lee MG, Choe SW, Lee MC, Chung HG, Cho SH
International journal of dermatology 2003 Feb;42(2):123-9
International journal of dermatology 2003 Feb;42(2):123-9
Acral lentiginous melanoma: an immunohistochemical study of 20 cases.
Kim YC, Lee MG, Choe SW, Lee MC, Chung HG, Cho SH
International journal of dermatology 2003 Feb;42(2):123-9
International journal of dermatology 2003 Feb;42(2):123-9
Hyperpigmentation during interferon-alpha therapy for chronic hepatitis C virus infection.
Willems M, Munte K, Vrolijk JM, Den Hollander JC, Böhm M, Kemmeren MH, De Man RA, Brouwer JT
The British journal of dermatology 2003 Aug;149(2):390-4
The British journal of dermatology 2003 Aug;149(2):390-4
Hyperpigmentation during interferon-alpha therapy for chronic hepatitis C virus infection.
Willems M, Munte K, Vrolijk JM, Den Hollander JC, Böhm M, Kemmeren MH, De Man RA, Brouwer JT
The British journal of dermatology 2003 Aug;149(2):390-4
The British journal of dermatology 2003 Aug;149(2):390-4
Comparison of five antibodies as markers in the diagnosis of melanoma in cytologic preparations.
Sheffield MV, Yee H, Dorvault CC, Weilbaecher KN, Eltoum IA, Siegal GP, Fisher DE, Chhieng DC
American journal of clinical pathology 2002 Dec;118(6):930-6
American journal of clinical pathology 2002 Dec;118(6):930-6
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot was performed using Anti-Melan-A Monoclonal Antibody (A103) (Product # MA5-14168) and a 17 kDa band corresponding to MLANA was observed in positive cell line SK-MEL-5 and not in negative cell lines A-375 and OVCAR3. Whole cell extracts (30 µg) of SK-MEL-5 (Lane 1), A-375 (Lane 2) and OVCAR-3 (Lane 3) were electrophoresed using NuPAGE™ 12% Bis-Tris Protein Gel (Product # NP0341BOX), 10 well. Resolved proteins were then transferred onto a nitrocellulose membrane (Product # IB23001) by iBlot® 2 Dry Blotting System (Product # IB21001). The blot was probed with the primary antibody (1:750 dilution) and detected by chemiluminescence with Goat anti-Mouse IgG (H+L) Superclonal™ Recombinant Secondary Antibody, HRP (Product # A28177, 1:20000 dilution) using the iBright™ FL1500 Imaging System (Product # A44115). Chemiluminescent detection was performed using SuperSignal™ West Pico PLUS Chemiluminescent Substrate (Product # 34580).
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Formalin-fixed, paraffin-embedded human melanoma stained with MART-1 antibody using peroxidase-conjugate and AEC chromogen. Note cytoplasmic staining of tumor cells.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical analysis of Melan-A was performed using formalin-fixed paraffin-embedded human skin (malignant melanoma) tissue sections. To expose the target protein, heat-induced epitope retrieval was performed on de-paraffinized sections using eBioscience™ IHC Antigen Retrieval Solution - Low pH (10X) (Product # 00-4955-58) diluted to 1X solution in water in a decloaking chamber at 110 degree Celsius for 15 minutes. Following antigen retrieval, the sections were blocked with 2% normal goat serum in 1X PBS for 45 minutes at room temperature and then probed with or without Melan-A Monoclonal Antibody (A103) (Product # MA5-14168) at 1:100 dilution in 0.1% normal goat serum overnight at 4 degree Celsius in a humidified chamber. Detection was performed using Goat anti-Mouse IgG (H+L) Highly Cross-Adsorbed Secondary Antibody, Alexa Fluor Plus 488 (Product # A32723) at a dilution of 1:2000 in 0.1% normal goat serum for 45 minutes at room temperature. ReadyProbes™ Tissue Autofluorescence Quenching Kit (Product # R37630) was used to quench autofluorescence from the tissues. Nuclei were stained with DAPI (Product # D1306) and the sections were mounted using ProLong™ Glass Antifade Mountant (Product # P36984). The images were captured on EVOS™ M7000 Imaging System (Product # AMF7000) at 20X magnification.
