Antibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [1]
- Immunocytochemistry [1]
- Immunohistochemistry [1]
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Validation data
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- Product number
- PA5-85106 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- PTHLH Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Recombinant full-length protein
- Description
- Keep as concentrated solution. Predicted reactivity: Mouse (85%), Rat (87%), Dog (92%), Pig (91%), Rabbit (87%), Rhesus Monkey (99%), Bovine (91%). Positive Control: A549, HepG2, HCT116. Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.
- Reactivity
- Human
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 100 µL
- Concentration
- 0.88 mg/mL
- Storage
- Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of PTHLH in A) HepG2, B) HCT116 cells using PTHLH polyclonal antibody (Product # PA5-85106) using 30 µg of sample at a dilution of 1:1000. Prior to incubation with primary antibody, the sample was separated on 12% SDS PAGE.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunocytochemistry analysis of PTHLH in 4% paraformaldehyde-fixed HepG2 cells using PTHLH polyclonal antibody (Product # PA5-85106) at a dilution of 1:500. Sample was then incubated with Hoechst secondary antibody.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemistry analysis of PTHLH in paraffin-embedded human lung carcinoma using PTHLH polyclonal antibody (Product # PA5-85106) at a dilution of 1:500.