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- Antibody Data
- Antigen structure
- References [1]
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- Product number
- 500-P276-100UG - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- PTHLH Polyclonal Antibody, PeproTech®
- Antibody type
- Polyclonal
- Antigen
- Recombinant full-length protein
- Description
- AA Sequence of recombinant protein: AVSEHQLLHD KGKSIQDLRR RFFLHHLIAE IHTAEIRATS EVSPNSKPSP NTKNHPVRFG SDDEGRYLTQ ETNKVETYKE QPLKTP. Preparation: Produced from sera of rabbits immunized with highly pure Recombinant Human PTHrP. Anti-Human PTHrP-specific antibody was purified by affinity chromatography employing an immobilized Human PTHrP matrix. Sandwich ELISA: To detect Human PTHrP by sandwich ELISA (using 100 µL/well antibody solution) a concentration of 0.5-2.0 µg/mL of this antibody is required. This antigen affinity purified antibody, in conjunction with PeproTech Biotinylated Anti-Human PTHrP (500-P276Bt) as a detection antibody, allows the detection of at least 0.2-0.4 ng/well of Recombinant Human PTHrP. Western Blot: To detect Human PTHrP by Western Blot analysis this antibody can be used at a concentration of 0.1-0.2 µg/mL. Used in conjunction with compatible secondary reagents the detection limit for Recombinant Human PTHrP is 1.5-3.0 ng/lane, under either reducing or non-reducing conditions.
- Reactivity
- Human
- Host
- Rabbit
- Vial size
- 100 µg
- Concentration
- 0.1-1.0 mg/mL
- Storage
- -20°C
Submitted references PTHrP induces STAT5 activation, secretory differentiation and accelerates mammary tumor development.
Grinman DY, Boras-Granic K, Takyar FM, Dann P, Hens JR, Marmol C, Lee J, Choi J, Chodosh LA, Sola MEG, Wysolmerski JJ
Breast cancer research : BCR 2022 Apr 19;24(1):30
Breast cancer research : BCR 2022 Apr 19;24(1):30
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Supportive validation
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- Invitrogen Antibodies (provider)
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- Experimental details
- Sandwich ELISA: To detect Human PTHrP by sandwich ELISA (using 100 µL/well antibody solution) a concentration of 0.5-2.0 µg/mL of PTHLH Polyclonal Antibody (Product # 500-P276-1MG) is required. This antigen affinity purified antibody, in conjunction with PeproTech PTHLH Polyclonal Antibody, Biotin (Product # 500-P276BT-1MG) as a detection antibody, allows the detection of at least 0.2-0.4 ng/well of Recombinant Human PTHrP.
