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Western blot
ImmunocytochemistryAntibody data
- Antibody Data
- Antigen structure
- References [9]
- Comments [0]
- Validations
- Western blot [1]
- Immunohistochemistry [2]
- Other assay [1]
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- Product number
- V2290 - Provider product page
- Provider
- NSJ Bioreagents
- Product name
- Tyrosinase Antibody
- Antibody type
- Monoclonal
- Antigen
- Recombinant Tyrosinase protein was used as the immunogen for this antibody.
- Description
- Protein G purified antibody
- Reactivity
- Human
- Host
- Mouse
- Conjugate
- Unconjugated
- Antibody clone number
- T311
- Vial size
- 20 µg, 100 µg (with sodium azide), 100 µg (without sodium azide), 7 ml (prediluted for IHC use, if applicable)
- Storage
- Store the Tyrosinase antibody at 2-8°C (with azide) or aliquot and store at -20°C or colder (without azide).
Submitted references Omeprazole, a gastric proton pump inhibitor, inhibits melanogenesis by blocking ATP7A trafficking.
Human tyrosinase produced in insect cells: a landmark for the screening of new drugs addressing its activity.
Key regulatory role of dermal fibroblasts in pigmentation as demonstrated using a reconstructed skin model: impact of photo-aging.
Novel delivery system for T-oligo using a nanocomplex formed with an alpha helical peptide for melanoma therapy.
Rhododendrol, a depigmentation-inducing phenolic compound, exerts melanocyte cytotoxicity via a tyrosinase-dependent mechanism.
Depigmentation caused by application of the active brightening material, rhododendrol, is related to tyrosinase activity at a certain threshold.
Human melanoma metastases demonstrate nonstochastic site-specific antigen heterogeneity that correlates with T-cell infiltration.
The usefulness of tyrosinase in the immunohistochemical assessment of melanocytic lesions: a comparison of the novel T311 antibody (anti-tyrosinase) with S-100, HMB45, and A103 (anti-melan-A).
T311--an anti-tyrosinase monoclonal antibody for the detection of melanocytic lesions in paraffin embedded tissues.
Matsui MS, Petris MJ, Niki Y, Karaman-Jurukovska N, Muizzuddin N, Ichihashi M, Yarosh DB
The Journal of investigative dermatology 2015 Mar;135(3):834-41
The Journal of investigative dermatology 2015 Mar;135(3):834-41
Human tyrosinase produced in insect cells: a landmark for the screening of new drugs addressing its activity.
Fogal S, Carotti M, Giaretta L, Lanciai F, Nogara L, Bubacco L, Bergantino E
Molecular biotechnology 2015 Jan;57(1):45-57
Molecular biotechnology 2015 Jan;57(1):45-57
Key regulatory role of dermal fibroblasts in pigmentation as demonstrated using a reconstructed skin model: impact of photo-aging.
Duval C, Cohen C, Chagnoleau C, Flouret V, Bourreau E, Bernerd F
PloS one 2014;9(12):e114182
PloS one 2014;9(12):e114182
Novel delivery system for T-oligo using a nanocomplex formed with an alpha helical peptide for melanoma therapy.
Uppada SB, Erickson T, Wojdyla L, Moravec DN, Song Z, Cheng J, Puri N
International journal of nanomedicine 2014;9:43-53
International journal of nanomedicine 2014;9:43-53
Rhododendrol, a depigmentation-inducing phenolic compound, exerts melanocyte cytotoxicity via a tyrosinase-dependent mechanism.
Sasaki M, Kondo M, Sato K, Umeda M, Kawabata K, Takahashi Y, Suzuki T, Matsunaga K, Inoue S
Pigment cell & melanoma research 2014 Sep;27(5):754-63
Pigment cell & melanoma research 2014 Sep;27(5):754-63
Depigmentation caused by application of the active brightening material, rhododendrol, is related to tyrosinase activity at a certain threshold.
Kasamatsu S, Hachiya A, Nakamura S, Yasuda Y, Fujimori T, Takano K, Moriwaki S, Hase T, Suzuki T, Matsunaga K
Journal of dermatological science 2014 Oct;76(1):16-24
Journal of dermatological science 2014 Oct;76(1):16-24
Human melanoma metastases demonstrate nonstochastic site-specific antigen heterogeneity that correlates with T-cell infiltration.
Bartlett EK, Fetsch PA, Filie AC, Abati A, Steinberg SM, Wunderlich JR, White DE, Stephens DJ, Marincola FM, Rosenberg SA, Kammula US
Clinical cancer research : an official journal of the American Association for Cancer Research 2014 May 15;20(10):2607-16
Clinical cancer research : an official journal of the American Association for Cancer Research 2014 May 15;20(10):2607-16
The usefulness of tyrosinase in the immunohistochemical assessment of melanocytic lesions: a comparison of the novel T311 antibody (anti-tyrosinase) with S-100, HMB45, and A103 (anti-melan-A).
Clarkson KS, Sturdgess IC, Molyneux AJ
Journal of clinical pathology 2001 Mar;54(3):196-200
Journal of clinical pathology 2001 Mar;54(3):196-200
T311--an anti-tyrosinase monoclonal antibody for the detection of melanocytic lesions in paraffin embedded tissues.
Jungbluth AA, Iversen K, Coplan K, Kolb D, Stockert E, Chen YT, Old LJ, Busam K
Pathology, research and practice 2000;196(4):235-42
Pathology, research and practice 2000;196(4):235-42
No comments: Submit comment
Supportive validation
- Submitted by
- NSJ Bioreagents (provider)
- Main image
- Experimental details
- Western blot testing of human A431 cell lysate with Tyrosinase antibody (clone T311). Expected molecular weight: ~60-84kDa depending on glycosylation level.
Supportive validation
- Submitted by
- NSJ Bioreagents (provider)
- Main image
- Experimental details
- IHC staining of melanoma tissue (10X) with Tyrosinase antibody (T311).
- Submitted by
- NSJ Bioreagents (provider)
- Main image
- Experimental details
- IHC staining of melanoma tissue (20X) with Tyrosinase antibody (T311).
Supportive validation
- Submitted by
- NSJ Bioreagents (provider)
- Main image
- Experimental details
- SDS-PAGE Analysis of Purified, BSA-Free Tyrosinase Antibody (clone T311). Confirmation of Integrity and Purity of the Antibody.
