Antibody data
- Antibody Data
- Antigen structure
- References [15]
- Comments [0]
- Validations
- Western blot [6]
- Immunocytochemistry [1]
- Immunoprecipitation [1]
- Immunohistochemistry [1]
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Validation data
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- Product number
- GTX103042 - Provider product page
- Provider
- GeneTex
- Proper citation
- GeneTex Cat#GTX103042, RRID:AB_1949884
- Product name
- Cyclin A2 antibody
- Antibody type
- Polyclonal
- Reactivity
- Human, Mouse, Rat
- Host
- Rabbit
Submitted references PDZRN3 regulates differentiation of myoblasts into myotubes through transcriptional and posttranslational control of Id2.
Upregulation of LGALS1 is associated with oral cancer metastasis.
Heat shock protein 27 influences the anti-cancer effect of curcumin in colon cancer cells through ROS production and autophagy activation.
A high-throughput pipeline for validation of antibodies.
Microarray analysis reveals Tmub1 as a cell cycle-associated protein in rat hepatocytes.
Aqueous extracts of Paeonia suffruticosa modulates mitochondrial proteostasis by reactive oxygen species-induced endoplasmic reticulum stress in pancreatic cancer cells.
ZFP36L1 and ZFP36L2 inhibit cell proliferation in a cyclin D-dependent and p53-independent manner.
Silencing Heat Shock Protein 27 Inhibits the Progression and Metastasis of Colorectal Cancer (CRC) by Maintaining the Stability of Stromal Interaction Molecule 1 (STIM1) Proteins.
Aqueous extract of Polygonum bistorta modulates proteostasis by ROS-induced ER stress in human hepatoma cells.
The transcriptional co-repressor TLE3 regulates myogenic differentiation by repressing the activity of the MyoD transcription factor.
PGRMC1 contributes to doxorubicin-induced chemoresistance in MES-SA uterine sarcoma.
Hinokitiol induces DNA damage and autophagy followed by cell cycle arrest and senescence in gefitinib-resistant lung adenocarcinoma cells.
The antiproliferative effect of C2-ceramide on lung cancer cells through apoptosis by inhibiting Akt and NFκB.
The C-terminus of PARK2 is required for its self-interaction, solubility and role in the spindle assembly checkpoint.
Suppression of cell-cycle progression by Jun dimerization protein-2 (JDP2) involves downregulation of cyclin-A2.
Honda T, Inui M
Journal of cellular physiology 2019 Mar;234(3):2963-2972
Journal of cellular physiology 2019 Mar;234(3):2963-2972
Upregulation of LGALS1 is associated with oral cancer metastasis.
Li JM, Tseng CW, Lin CC, Law CH, Chien YA, Kuo WH, Chou HC, Wang WC, Chan HL
Therapeutic advances in medical oncology 2018;10:1758835918794622
Therapeutic advances in medical oncology 2018;10:1758835918794622
Heat shock protein 27 influences the anti-cancer effect of curcumin in colon cancer cells through ROS production and autophagy activation.
Liang HH, Huang CY, Chou CW, Makondi PT, Huang MT, Wei PL, Chang YJ
Life sciences 2018 Sep 15;209:43-51
Life sciences 2018 Sep 15;209:43-51
A high-throughput pipeline for validation of antibodies.
Sikorski K, Mehta A, Inngjerdingen M, Thakor F, Kling S, Kalina T, Nyman TA, Stensland ME, Zhou W, de Souza GA, Holden L, Stuchly J, Templin M, Lund-Johansen F
Nature methods 2018 Nov;15(11):909-912
Nature methods 2018 Nov;15(11):909-912
Microarray analysis reveals Tmub1 as a cell cycle-associated protein in rat hepatocytes.
Fu H, Xu J, Chen J, Li G, Zhao X, Chen P
Molecular medicine reports 2018 Mar;17(3):4337-4344
Molecular medicine reports 2018 Mar;17(3):4337-4344
Aqueous extracts of Paeonia suffruticosa modulates mitochondrial proteostasis by reactive oxygen species-induced endoplasmic reticulum stress in pancreatic cancer cells.
Liu YH, Weng YP, Tsai HY, Chen CJ, Lee DY, Hsieh CL, Wu YC, Lin JY
Phytomedicine : international journal of phytotherapy and phytopharmacology 2018 Jul 15;46:184-192
Phytomedicine : international journal of phytotherapy and phytopharmacology 2018 Jul 15;46:184-192
ZFP36L1 and ZFP36L2 inhibit cell proliferation in a cyclin D-dependent and p53-independent manner.
Suk FM, Chang CC, Lin RJ, Lin SY, Liu SC, Jau CF, Liang YC
Scientific reports 2018 Feb 9;8(1):2742
Scientific reports 2018 Feb 9;8(1):2742
Silencing Heat Shock Protein 27 Inhibits the Progression and Metastasis of Colorectal Cancer (CRC) by Maintaining the Stability of Stromal Interaction Molecule 1 (STIM1) Proteins.
Huang CY, Wei PL, Chen WY, Chang WC, Chang YJ
Cells 2018 Dec 10;7(12)
Cells 2018 Dec 10;7(12)
Aqueous extract of Polygonum bistorta modulates proteostasis by ROS-induced ER stress in human hepatoma cells.
Liu YH, Weng YP, Lin HY, Tang SW, Chen CJ, Liang CJ, Ku CY, Lin JY
Scientific reports 2017 Jan 30;7:41437
Scientific reports 2017 Jan 30;7:41437
The transcriptional co-repressor TLE3 regulates myogenic differentiation by repressing the activity of the MyoD transcription factor.
Kokabu S, Nakatomi C, Matsubara T, Ono Y, Addison WN, Lowery JW, Urata M, Hudnall AM, Hitomi S, Nakatomi M, Sato T, Osawa K, Yoda T, Rosen V, Jimi E
The Journal of biological chemistry 2017 Aug 4;292(31):12885-12894
The Journal of biological chemistry 2017 Aug 4;292(31):12885-12894
PGRMC1 contributes to doxorubicin-induced chemoresistance in MES-SA uterine sarcoma.
Lin ST, May EW, Chang JF, Hu RY, Wang LH, Chan HL
Cellular and molecular life sciences : CMLS 2015 Jun;72(12):2395-409
Cellular and molecular life sciences : CMLS 2015 Jun;72(12):2395-409
Hinokitiol induces DNA damage and autophagy followed by cell cycle arrest and senescence in gefitinib-resistant lung adenocarcinoma cells.
Li LH, Wu P, Lee JY, Li PR, Hsieh WY, Ho CC, Ho CL, Chen WJ, Wang CC, Yen MY, Yang SM, Chen HW
PloS one 2014;9(8):e104203
PloS one 2014;9(8):e104203
The antiproliferative effect of C2-ceramide on lung cancer cells through apoptosis by inhibiting Akt and NFκB.
Lin IL, Chou HL, Lee JC, Chen FW, Fong Y, Chang WC, Huang HW, Wu CY, Chang WT, Wang HD, Chiu CC
Cancer cell international 2014 Jan 6;14(1):1
Cancer cell international 2014 Jan 6;14(1):1
The C-terminus of PARK2 is required for its self-interaction, solubility and role in the spindle assembly checkpoint.
Chen Y, Fang ST, Yeh PC, Yang HH, Chen SY, Chang CJ, Zhai WJ, Chen YC, Juang YL
Biochimica et biophysica acta 2012 Apr;1822(4):573-80
Biochimica et biophysica acta 2012 Apr;1822(4):573-80
Suppression of cell-cycle progression by Jun dimerization protein-2 (JDP2) involves downregulation of cyclin-A2.
Pan J, Nakade K, Huang YC, Zhu ZW, Masuzaki S, Hasegawa H, Murata T, Yoshiki A, Yamaguchi N, Lee CH, Yang WC, Tsai EM, Obata Y, Yokoyama KK
Oncogene 2010 Nov 25;29(47):6245-56
Oncogene 2010 Nov 25;29(47):6245-56
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Supportive validation
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- Cyclin A2 antibody detects CCNA2 protein by western blot analysis.A. 30 ?g Neuro2A whole cell lysate/extract 10% SDS-PAGECyclin A2 antibody (GTX103042) dilution: 1:1000 The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
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- Cyclin A2 antibody detects CCNA2 protein by western blot analysis.A. 30 ?g PC-12 whole cell lysate/extract 10% SDS-PAGECyclin A2 antibody (GTX103042) dilution: 1:1000 The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
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- Cyclin A2 antibody detects CCNA2 protein by western blot analysis.A. 30 ?g 293T whole cell lysate/extractB. 30 ?g HeLa whole cell lysate/extractC. 30 ?g A375 whole cell lysate/extract10% SDS-PAGECyclin A2 antibody (GTX103042) dilution: 1:1000 The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
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- Cyclin A2 antibody detects Cyclin A2 protein by western blot analysis. Various whole cell extracts (30 ?g) were separated by 10% SDS-PAGE, and the membrane was blotted with Cyclin A2 antibody (GTX103042) diluted at a dilution of 1:1000.
- Validation comment
- WB
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- Cyclin A2 antibody detects Cyclin A2 protein by western blot analysis. Various whole cell extracts (30 ?g) were separated by 10% SDS-PAGE, and the membrane was blotted with Cyclin A2 antibody (GTX103042) diluted at a dilution of 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
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- Main image
- Experimental details
- Untreated (¡V) and treated (+) HeLa whole cell extracts (30 ?g) were separated by 10% SDS-PAGE, and the membrane was blotted with Cyclin A2 antibody (GTX103042) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
Supportive validation
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- Cyclin A2 antibody detects Cyclin A2 protein at cytoplasm and nucleus by immunofluorescent analysis.Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min.Green: Cyclin A2 protein stained by Cyclin A2 antibody (GTX103042) diluted at 1:200.Red: phalloidin, a cytoskeleton marker, stained by phalloidin (invitrogen, A12380) diluted at 1:200.Blue: Hoechst 33342 staining.
Supportive validation
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- Cyclin A2 antibody immunoprecipitates cyclin A2 protein in IP experiments. IP Sample: 1000 £gg 293T whole cell lysate/extract A. 30 £gg 293T whole cell lysate/extract B. Control with 2.5 £gg of preimmune rabbit IgG C. Immunoprecipitation of Cyclin A2 protein by 2.5 £gg of Cyclin A2 antibody (GTX103042) 10% SDS-PAGE The immunoprecipitated Cyclin A2 protein was detected by Cyclin A2 antibody (GTX103042) diluted at 1:1000. EasyBlot anti-rabbit IgG (GTX221666-01) was used as a secondary reagent.
Supportive validation
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- Immunohistochemical analysis of paraffin-embedded NCIN87 Xenograft, using cyclin A (GTX103042) antibody at 1:500 dilution.