Antibody data
- Antibody Data
- Antigen structure
- References [4]
- Comments [0]
- Validations
- Western blot [1]
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Validation data
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- Product number
- AF5999 - Provider product page
- Provider
- Novus Biologicals
- Product name
- Goat Polyclonal Cyclin A2 Antibody
- Antibody type
- Polyclonal
- Description
- Antigen Affinity-purified. Detects human and mouse Cyclin A2 in Western blots.
- Reactivity
- Human, Mouse
- Host
- Goat
- Conjugate
- Unconjugated
- Isotype
- IgG
- Vial size
- 100 ug
- Concentration
- LYOPH
- Storage
- Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 degreesC as supplied. 1 month, 2 to 8 degreesC under sterile conditions after reconstitution. 6 months, -20 to -70 degreesC under sterile conditions after reconstitution.
Submitted references Cisplatin exposure causes c-Myc-dependent resistance to CDK4/6 inhibition in HPV-negative head and neck squamous cell carcinoma.
Chemotherapy-induced differential cell cycle arrest in B-cell lymphomas affects their sensitivity to Wee1 inhibition.
CREB is activated by muscle injury and promotes muscle regeneration.
Chemerin, a novel peroxisome proliferator-activated receptor gamma (PPARgamma) target gene that promotes mesenchymal stem cell adipogenesis.
Robinson AM, Rathore R, Redlich NJ, Adkins DR, VanArsdale T, Van Tine BA, Michel LS
Cell death & disease 2019 Nov 14;10(11):867
Cell death & disease 2019 Nov 14;10(11):867
Chemotherapy-induced differential cell cycle arrest in B-cell lymphomas affects their sensitivity to Wee1 inhibition.
Wang X, Chen Z, Mishra AK, Silva A, Ren W, Pan Z, Wang JH
Haematologica 2018 Mar;103(3):466-476
Haematologica 2018 Mar;103(3):466-476
CREB is activated by muscle injury and promotes muscle regeneration.
Stewart R, Flechner L, Montminy M, Berdeaux R
PloS one 2011;6(9):e24714
PloS one 2011;6(9):e24714
Chemerin, a novel peroxisome proliferator-activated receptor gamma (PPARgamma) target gene that promotes mesenchymal stem cell adipogenesis.
Muruganandan S, Parlee SD, Rourke JL, Ernst MC, Goralski KB, Sinal CJ
The Journal of biological chemistry 2011 Jul 8;286(27):23982-95
The Journal of biological chemistry 2011 Jul 8;286(27):23982-95
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Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Detection of Human and Mouse Cyclin A2 by Western Blot. Western blot shows lysates of HeLa human cervical epithelial carcinoma cell line, C2C12 mouse myoblast cell line, and NIH-3T3 mouse embryonic fibroblast cell line. PVDF Membrane was probed with 0.5 µg/mL of Goat Anti-Human/Mouse Cyclin A2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF5999) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF017). A specific band was detected for Cyclin A2 at approximately 60 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.