PB9424

antibody from Boster Biological Technology

Targeting: CCNA2 CCN1, CCNA

Western blot (Supportive data in Antibodypedia)

Immunocytochemistry (Data presented on provider website)

Immunohistochemistry (Data presented on provider website)

Flow cytometry (Data presented on provider website)

Antibody Data

Product number

PB9424

Provider

Boster Biological Technology

Product name

Anti-Cyclin A2/CCNA2 Antibody Picoband™

Antibody type

Polyclonal

Description

Polyclonal antibody for CYCLIN A/CCNA2 detection. Host: Rabbit.Size: 100μg/vial. Tested applications: WB, IHC-P, IHC-F, ICC/IF, FCM. Reactive species: Human. CYCLIN A/CCNA2 information: Molecular Weight: 48551 MW; Subcellular Localization: Nucleus . Cytoplasm . Cytoplasmic when associated with SCAPER.

Reactivity

Human, Rat

Host

Rabbit

Vial size

100μg/vial

Concentration

Add 0.2ml of distilled water will yield a concentration of 500ug/ml.

Storage

At -20°C for one year. After reconstitution, at 4°C for one month. It can also be aliquoted and stored frozen at -20°C for a longer time. Avoid repeated freezing and thawing.

Handling

Add 0.2ml of distilled water will yield a concentration of 500ug/ml.

References

Submitted references

IL-2Rα up-regulation is mediated by latent membrane protein 1 and promotes lymphomagenesis and chemotherapy resistance in natural killer/T-cell lymphoma.

Wang L, Bi XW, Zhu YJ, He YZ, Lai QY, Xia ZJ, Cai QQ
Cancer communications (London, England) 2018 Oct 19;38(1):62

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The novel protein C3orf43 accelerates hepatocyte proliferation.

Zhang C, Chang C, Li D, Zhang F, Xu C
Cellular & molecular biology letters 2017;22:21

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The novel protein C9orf116 promotes rat liver cell line BRL-3A proliferation.

Zhang C, Chang C, Zhao W, Gao H, Wang Q, Li D, Zhang F, Zhang S, Xu C
PloS one 2017;12(7):e0180607

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Synergism between thioredoxin reductase inhibitor ethaselen and sodium selenite in inhibiting proliferation and inducing death of human non-small cell lung cancer cells.

Zheng X, Xu W, Sun R, Yin H, Dong C, Zeng H
Chemico-biological interactions 2017 Sep 25;275:74-85

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A New Double Stranded RNA Suppresses Bladder Cancer Development by Upregulating p21 (Waf1/CIP1) Expression.

Wang C, Ge Q, Chen Z, Hu J, Li F, Song X, Xu H, Ye Z
BioMed research international 2015;2015:304753

Western blot

Supportive validation

Submitted by

Boster Biological Technology (provider)

Main image

Experimental details

Western blot analysis of Cyclin A2 using anti-Cyclin A2 antibody (PB9424). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: Rat Skeletal Muscle Tissue Lysate Lane 2: HELA Whole Cell Lysate Lane 3: COLO320 Whole Cell Lysate Lane 4: HEPG2 Whole Cell Lysate Lane 5: MCF-7 Whole Cell Lysate After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Cyclin A2 antigen affinity purified polyclonal antibody (Catalog # PB9424) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for Cyclin A2 at approximately 49KD. The expected band size for Cyclin A2 is at 49KD.

Additional image