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Western blot
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- Antibody Data
- Antigen structure
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- Validations
- Western blot [8]
- Immunocytochemistry [1]
- Immunohistochemistry [1]
- Other assay [1]
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- Product number
- PA5-34682 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- Cyclin A2 Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Recombinant protein fragment
- Description
- Recommended positive controls: 293T, A431, HeLa, HepG2, IMR32, SK-N-AS, HeLa(100 µg/mL Bleomycin treatment for 24 hr), Neuro2A, PC-12, PC3. Predicted reactivity: Rat (80%), Pig (89%), Rabbit (84%), Rhesus Monkey (98%), Bovine (84%). Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.
- Reactivity
- Human, Mouse, Rat
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 100 µL
- Concentration
- 0.73 mg/mL
- Storage
- Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
Submitted references Fibroblast Growth Factor 12 Is a Novel Regulator of Vascular Smooth Muscle Cell Plasticity and Fate.
Song SH, Kim K, Jo EK, Kim YW, Kwon JS, Bae SS, Sung JH, Park SG, Kim JT, Suh W
Arteriosclerosis, thrombosis, and vascular biology 2016 Sep;36(9):1928-36
Arteriosclerosis, thrombosis, and vascular biology 2016 Sep;36(9):1928-36
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of Cyclin A2 using A) 30 µg 293T whole cell lysate (B) 30 µg HeLa whole cell lysate and C) 30 µg A375 whole cell lysate. Samples were loaded onto a 10% SDS-PAGE gel and probed with a Cyclin A2 polyclonal antibody (Product # PA5-34682) at a dilution of 1:1000.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western Blot analysis of Cyclin A2 was performed by separating 30 µg of various whole cell extracts by 10% SDS-PAGE. Proteins were transferred to a membrane and probed with a Cyclin A2 Polyclonal Antibody (Product # PA5-34682) at a dilution of 1:1000 and a HRP-conjugated anti-rabbit IgG secondary antibody.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western Blot analysis of Cyclin A2 was performed by separating 30 µg of untreated (–) and treated (+) HeLa whole cell extracts by 10% SDS-PAGE. Proteins were transferred to a membrane and probed with a Cyclin A2 Polyclonal Antibody (Product # PA5-34682) at a dilution of 1:1000.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western Blot using Cyclin A2 Polyclonal Antibody (Product # PA5-34682). Various whole cell extracts (30 µg) were separated by 10% SDS-PAGE, and the membrane was blotted with Cyclin A2 Polyclonal Antibody (Product # PA5-34682) diluted at 1:1,000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Cyclin A2 Polyclonal Antibody detects CCNA2 protein by western blot analysis. A. 30 µg Neuro2A whole cell lysate/extract.10% SDS-PAGE. Cyclin A2 Polyclonal Antibody (Product # PA5-34682) dilution: 1:1,000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Cyclin A2 Polyclonal Antibody detects CCNA2 protein by western blot analysis. A. 30 µg PC-12 whole cell lysate/extract.10% SDS-PAGE. Cyclin A2 Polyclonal Antibody (Product # PA5-34682) dilution: 1:1,000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Knockdown of Cyclin A2 was achieved by transfecting HeLa cells with Cyclin A2 specific siRNAs (Silencer® select Product # s2514, s2512). Western blot analysis (Fig. a) was performed using whole cell extracts from the Cyclin A2 knockdown cells (lane 3), non-specific scrambled siRNA transfected cells (lane 2) and untransfected cells (lane 1). The blots were probed with Cyclin A2 Polyclonal Antibody (Product # PA5-34682, 1:3000 dilution) and Goat anti-Rabbit IgG (H+L) Superclonal™ Secondary Antibody, HRP conjugate (Product # A27036, 0.25 µg/ml, 1:4000 dilution). Densitometric analysis of this western blot is shown in histogram (Fig. b). Decrease in signal upon siRNA mediated knock down confirms that antibody is specific to Cyclin A2.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis was performed on whole cell extract (30 µg lysate) of HeLa (Lane 1), HeLa treated with doxorubicin (0.5uM, 24 hrs) (Lane 2), COS-7 (Lane 3), A431 (Lane 4) and Jeko1 (Lane 5). The blot was probed with Anti-Cyclin A2 Polyclonal Antibody (Product # PA5-34682, 1:3000 dilution) and detected by chemiluminescence using Goat anti-Rabbit IgG (H+L) Superclonal™ Secondary Antibody, HRP conjugate (Product # A27036, 0.25 µg/ml, 1:4000 dilution). A 52 kDa band corresponding to Cyclin A2 was detected in cell lines tested and was increased upon Doxorubicin treatment in HeLa cell line. An additional non-specific band at ~20kDa was also detected across cell lines tested.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunocytochemistry-Immunofluorescence analysis of Cyclin A2 was performed in HeLa cells fixed in 4% paraformaldehyde at RT for 15 min. Green: Cyclin A2 Polyclonal Antibody (Product # PA5 34682) diluted at 1:500. Red: phalloidin, a cytoskeleton marker. Scale bar = 10 µm.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical analysis of paraffin-embedded NCIN87 Xenograft, using cyclin A (Product # PA5-34682) antibody at 1:500 dilution. Antigen Retrieval: EDTA based buffer, pH 8.0, 15 min.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Cyclin A2 Polyclonal Antibody immunoprecipitates cyclin A2 protein in IP experiments. IP Sample: 1,000 µg 293T whole cell lysate/extract A. 30 µg 293T whole cell lysate/extract B. Control with 2.5 µg of preimmune rabbit IgG C. Immunoprecipitation of Cyclin A2 protein by 2.5 µg of Cyclin A2 Polyclonal Antibody (Product # PA5-34682) 10% SDS-PAGE The immunoprecipitated Cyclin A2 protein was detected by Cyclin A2 Polyclonal Antibody (Product # PA5-34682) diluted at 1:1,000.
