Antibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [1]
- Immunohistochemistry [1]
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Validation data
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- Product number
- TA319146 - Provider product page
- Provider
- OriGene
- Product name
- Rabbit polyclonal anti-Cyclin A antibody
- Antibody type
- Polyclonal
- Description
- Rabbit polyclonal anti-Cyclin A antibody
- Host
- Rabbit
- Conjugate
- Unconjugated
- Epitope
- CCNA2
- Isotype
- IgG
- Antibody clone number
- NULL
- Vial size
- 100 µl
- Concentration
- NULL
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Supportive validation
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- Western Blot of Rabbit anti- Cyclin A antibody. Lane 1: T98G cells treated with normal (n). Lane 2: T98G cells treated with scrambled (scr). Lane 3: T98G cells treated with PIN1 knockdown (kd1). Load: 25 ug per lane. Primary antibody: Cyclin A antibody at 1:400 for overnight at 4°C. Secondary antibody: IRDye800? rabbit secondary antibody at 1:10,000 for 45 min at RT. Block: 5% BLOTTO overnight at 4°C. Predicted/Observed size: 60kDa for Cyclin A.
- Validation comment
- WB
Supportive validation
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- Immunohistochemistry of Rabbit Anti-Cyclin A antibody. Tissue: human testes tissue. Fixation: formalin fixed paraffin embedded. Antigen retrieval: not required. Primary antibody: Cyclin A antibody at 1:500 for 1 h at RT. Secondary antibody: Peroxidase rabbit secondary antibody at 1:10,000 for 45 min at RT. Localization: Cyclin A is nuclear and cytoplasmic. Staining: Cyclin A as precipitated red signal with hematoxylin purple nuclear counterstain.
- Validation comment
- IHC