TA319146

antibody from OriGene

Targeting: CCNA2 CCN1, CCNA

Western blot (Supportive data in Antibodypedia)

Immunohistochemistry (Supportive data in Antibodypedia)

Antibody Data

Product number

TA319146

Provider

OriGene

Product name

Rabbit polyclonal anti-Cyclin A antibody

Antibody type

Polyclonal

Description

Rabbit polyclonal anti-Cyclin A antibody

Host

Rabbit

Conjugate

Unconjugated

Epitope

CCNA2

Isotype

IgG

Antibody clone number

NULL

Vial size

100 µl

Concentration

NULL

Western blot

Supportive validation

Submitted by

OriGene (provider)

Main image

Experimental details

Western Blot of Rabbit anti- Cyclin A antibody. Lane 1: T98G cells treated with normal (n). Lane 2: T98G cells treated with scrambled (scr). Lane 3: T98G cells treated with PIN1 knockdown (kd1). Load: 25 ug per lane. Primary antibody: Cyclin A antibody at 1:400 for overnight at 4°C. Secondary antibody: IRDye800„? rabbit secondary antibody at 1:10,000 for 45 min at RT. Block: 5% BLOTTO overnight at 4°C. Predicted/Observed size: 60kDa for Cyclin A.

Validation comment

WB

Immunohistochemistry

Supportive validation

Submitted by

OriGene (provider)

Main image

Experimental details

Immunohistochemistry of Rabbit Anti-Cyclin A antibody. Tissue: human testes tissue. Fixation: formalin fixed paraffin embedded. Antigen retrieval: not required. Primary antibody: Cyclin A antibody at 1:500 for 1 h at RT. Secondary antibody: Peroxidase rabbit secondary antibody at 1:10,000 for 45 min at RT. Localization: Cyclin A is nuclear and cytoplasmic. Staining: Cyclin A as precipitated red signal with hematoxylin purple nuclear counterstain.

Validation comment

IHC