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LearnMA5-17276
antibody from Invitrogen Antibodies
Targeting: TAGLN
DKFZp686P11128, SM22, SMCC, TAGLN1, WS3-10
Western blotAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [5]
- Immunocytochemistry [1]
- Immunohistochemistry [4]
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- Product number
- MA5-17276 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- TAGLN Monoclonal Antibody (GT336)
- Antibody type
- Monoclonal
- Antigen
- Recombinant protein fragment
- Description
- Recommended positive controls: HeLa, HepG2, C8D30, Rat2.
- Antibody clone number
- GT336
- Concentration
- 1 mg/mL
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western Blot using TAGLN Monoclonal Antibody (GT336) (Product # MA5-17276). Various whole cell extracts (30 µg) were separated by 12% SDS-PAGE, and the membrane was blotted with TAGLN Monoclonal Antibody (GT336) (Product # MA5-17276) diluted at 1:1,000. The HRP-conjugated anti-mouse IgG antibody was used to detect the primary antibody.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- TAGLN Polyclonal Antibody detects TAGLN protein by Western blot analysis. A. 30 µg C8D30 whole cell lysate/extract.12 % SDS-PAGE. TAGLN Polyclonal Antibody TAGLN Monoclonal Antibody (GT336) (Product # MA5-17276) dilution: 1:1,000.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- TAGLN Polyclonal Antibody detects TAGLN protein by Western blot analysis. A. 30 µg HeLa whole cell lysate/extract. B. 30 µg HepG2 whole cell lysate/extract.12 % SDS-PAGE. TAGLN Polyclonal Antibody TAGLN Monoclonal Antibody (GT336) (Product # MA5-17276) dilution: 1:1,000.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- TAGLN Polyclonal Antibody detects TAGLN protein by Western blot analysis. A. 30 µg Rat-2 whole cell lysate/extract.12 % SDS-PAGE. TAGLN Polyclonal Antibody TAGLN Monoclonal Antibody (GT336) (Product # MA5-17276) dilution: 1:1,000.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot was performed using Anti-TAGLN Monoclonal Antibody (GT336) (Product # MA5-17276) and 23 and 20 kDa bands corresponding to TAGLN were observed across all cell lines and tissues tested except Jurkat, Raji, Rat Skeletal Muscle and Rat Brain which are known to be low expressing (DOI: 10.1006/geno.1998.5267, 10.1371/journal.pone.0156429). Whole cell extracts (50 µg lysate) of BJ (Lane 1), Caco-2 (Lane 2), HeLa (Lane 3), Jurkat (Lane 4), Raji (Lane 5), Rat Urinary Bladder (Lane 6), Rat Uterus (Lane 7), Rat Skeletal Muscle (Lane 8), Rat Brain (Lane 9) were electrophoresed using NuPAGE™ 4-12% Bis-Tris Protein Gel (Product # NP0321BOX). Resolved proteins were then transferred onto a Nitrocellulose membrane (Product # IB23001) by iBlot® 2 Dry Blotting System (Product # IB21001). The blot was probed with the primary antibody (2 µg/mL) and detected by chemiluminescence with Goat anti-Mouse IgG (H+L) Superclonal™ Recombinant Secondary Antibody, HRP (Product # A28177, 1:4000 dilution) using the iBright FL 1000 (Product # A32752). Chemiluminescent detection was performed using SuperSignal™ West Dura Extended Duration Substrate (Product # 34076).Expression of TAGLN was observed in BJ, Caco-2, HeLa, Rat Urinary Bladder and Rat Uterus but not in Jurkat, Raji, Rat Skeletal Muscle and Rat Brain which are known to be low expressing.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunocytochemistry-Immunofluorescence analysis of TAGLN was performed in HeLa cells fixed in 4% paraformaldehyde at RT for 15 min. Green: TAGLN Monoclonal Antibody (GT336) (Product # MA5-17276) diluted at 1:500. Blue: Hoechst 33342 staining.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemistry (Paraffin) analysis of TAGLN was performed in paraffin-embedded mouse prostate tissue using TAGLN Monoclonal Antibody (GT336) (Product # MA5-17276) at a dilution of 1:500.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemistry (Paraffin) analysis of TAGLN was performed in paraffin-embedded rat kidney tissue using TAGLN Monoclonal Antibody (GT336) (Product # MA5-17276) at a dilution of 1:500.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- TAGLN Monoclonal Antibody (GT336) detects Transgelin protein at cytoplasm by immunohistochemical analysis. Sample: Paraffin-embedded mouse smooth muscle. Transgelin stained by TAGLN Monoclonal Antibody (GT336) (Product # MA5-17276) diluted at 1:200. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- TAGLN Monoclonal Antibody (GT336) detects Transgelin protein at cytoplasm by immunohistochemical analysis. Sample: Paraffin-embedded rat smooth muscle. Transgelin stained by TAGLN Monoclonal Antibody (GT336) (Product # MA5-17276) diluted at 1:200. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min.
