MA5-17288

antibody from Invitrogen Antibodies

Targeting: TAGLN DKFZp686P11128, SM22, SMCC, TAGLN1, WS3-10

Antibody data

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Validation data

Product number: MA5-17288 - Provider product page
Provider: Invitrogen Antibodies
Product name: TAGLN Monoclonal Antibody (GT4511)
Antibody type: Monoclonal
Antigen: Recombinant protein fragment
Description: Recommended positive controls: 293T, A431, HeLa, HepG2. Predicted reactivity: Mouse (96%), Rat (96%), Pig (98%), Chicken (85%), Bovine (97%). Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.
Reactivity: Human, Mouse, Rat
Host: Mouse
Isotype: IgG
Antibody clone number: GT4511
Vial size: 100 µL
Concentration: 1 mg/mL
Storage: Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

TAGLN protein structure - MA5-17288 shown in red.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Western blot analysis of Transgelin using 30 µg HeLa whole cell lysate. Samples were loaded onto a 12% SDS-PAGE gel and probed with a Transgelin monoclonal antibody (Product # MA5-17288) at a dilution of 1:1000.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Western Blot analysis of TAGLN was performed by separating 30 µg of various whole cell extracts by 12% SDS-PAGE. Proteins were transferred to a membrane and probed with a TAGLN Monoclonal Antibody (GT4511) (Product # MA5-17288) at a dilution of 1:1000 and a HRP-conjugated anti-mouse IgG secondary antibody.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Western Blot analysis of TAGLN was performed by separating 30 µg of various whole cell extracts by 12% SDS-PAGE. Proteins were transferred to a membrane and probed with a TAGLN Monoclonal Antibody (GT4511) (Product # MA5-17288) at a dilution of 1:1000 and a HRP-conjugated anti-mouse IgG secondary antibody.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Western blot was performed using Anti-TAGLN Monoclonal Antibody (GT4511) (Product # MA5-17288) and 23kDa band corresponding to TAGLN was observed across all cell lines and tissues tested except Jurkat, Raji, Rat Skeletal Muscle and Rat Brain which are known to be low expressing (DOI: 10.1006/geno.1998.5267, 10.1371/journal.pone.0156429). Whole cell extracts (50 µg lysate) of BJ (Lane 1), Caco-2 (Lane 2), HeLa (Lane 3), Jurkat (Lane 4), Raji (Lane 5), Rat Urinary Bladder (Lane 6), Rat Uterus (Lane 7), Rat Skeletal Muscle (Lane 8) and Rat Brain (Lane 9) were electrophoresed using NuPAGE™ 4-12% Bis-Tris Protein Gel (Product # NP0321BOX). Resolved proteins were then transferred onto a Nitrocellulose membrane (Product # IB23001) by iBlot® 2 Dry Blotting System (Product # IB21001). The blot was probed with the primary antibody (1:500) and detected by chemiluminescence with Goat anti-Mouse IgG (H+L) Superclonal™ Recombinant Secondary Antibody, HRP (Product # A28177, 1:4000) using the iBright FL 1000 (Product # A32752). Chemiluminescent detection was performed using SuperSignal™ West Dura Extended Duration Substrate (Product # 34076). Expression of TAGLN was observed in BJ, Caco-2, HeLa, Rat Urinary Bladder and Rat Uterus but not in Jurkat, Raji, Rat Skeletal Muscle and Rat Brain which are known to be low expressing.