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LearnMA5-17288
antibody from Invitrogen Antibodies
Targeting: TAGLN
DKFZp686P11128, SM22, SMCC, TAGLN1, WS3-10
Western blotAntibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [4]
- Immunohistochemistry [2]
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- Product number
- MA5-17288 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- TAGLN Monoclonal Antibody (GT4511)
- Antibody type
- Monoclonal
- Antigen
- Recombinant protein fragment
- Description
- Recommended positive controls: 293T, A431, HeLa, HepG2.
- Antibody clone number
- GT4511
- Concentration
- 1 mg/mL
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of Transgelin using 30 µg HeLa whole cell lysate. Samples were loaded onto a 12% SDS-PAGE gel and probed with a Transgelin monoclonal antibody (Product # MA5-17288) at a dilution of 1:1000.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western Blot analysis of TAGLN was performed by separating 30 µg of various whole cell extracts by 12% SDS-PAGE. Proteins were transferred to a membrane and probed with a TAGLN Monoclonal Antibody (GT4511) (Product # MA5-17288) at a dilution of 1:1000 and a HRP-conjugated anti-mouse IgG secondary antibody.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western Blot analysis of TAGLN was performed by separating 30 µg of various whole cell extracts by 12% SDS-PAGE. Proteins were transferred to a membrane and probed with a TAGLN Monoclonal Antibody (GT4511) (Product # MA5-17288) at a dilution of 1:1000 and a HRP-conjugated anti-mouse IgG secondary antibody.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot was performed using Anti-TAGLN Monoclonal Antibody (GT4511) (Product # MA5-17288) and 23kDa band corresponding to TAGLN was observed across all cell lines and tissues tested except Jurkat, Raji, Rat Skeletal Muscle and Rat Brain which are known to be low expressing (DOI: 10.1006/geno.1998.5267, 10.1371/journal.pone.0156429). Whole cell extracts (50 µg lysate) of BJ (Lane 1), Caco-2 (Lane 2), HeLa (Lane 3), Jurkat (Lane 4), Raji (Lane 5), Rat Urinary Bladder (Lane 6), Rat Uterus (Lane 7), Rat Skeletal Muscle (Lane 8) and Rat Brain (Lane 9) were electrophoresed using NuPAGE™ 4-12% Bis-Tris Protein Gel (Product # NP0321BOX). Resolved proteins were then transferred onto a Nitrocellulose membrane (Product # IB23001) by iBlot® 2 Dry Blotting System (Product # IB21001). The blot was probed with the primary antibody (1:500) and detected by chemiluminescence with Goat anti-Mouse IgG (H+L) Superclonal™ Recombinant Secondary Antibody, HRP (Product # A28177, 1:4000) using the iBright FL 1000 (Product # A32752). Chemiluminescent detection was performed using SuperSignal™ West Dura Extended Duration Substrate (Product # 34076). Expression of TAGLN was observed in BJ, Caco-2, HeLa, Rat Urinary Bladder and Rat Uterus but not in Jurkat, Raji, Rat Skeletal Muscle and Rat Brain which are known to be low expressing.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemistry (Paraffin) analysis of TAGLN was performed in paraffin-embedded mouse prostate tissue using TAGLN Monoclonal Antibody (GT4511) (Product # MA5-17288) at a dilution of 1:500.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemistry (Paraffin) analysis of TAGLN was performed in paraffin-embedded rat kidney tissue using TAGLN Monoclonal Antibody (GT4511) (Product # MA5-17288) at a dilution of 1:500.
