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LearnPA5-27463
antibody from Invitrogen Antibodies
Targeting: TAGLN
DKFZp686P11128, SM22, SMCC, TAGLN1, WS3-10
Western blot
ImmunocytochemistryAntibody data
- Antibody Data
- Antigen structure
- References [1]
- Comments [0]
- Validations
- Immunocytochemistry [2]
- Other assay [1]
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- Product number
- PA5-27463 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- TAGLN Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Recombinant protein fragment
- Description
- Recommended positive controls: 293T, A431, HeLa, HepG2, C8D30, Rat-2. Predicted reactivity: Mouse (96%), Rat (96%), Pig (98%), Chicken (85%), Bovine (97%). Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.
- Reactivity
- Human, Mouse, Rat
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 100 µL
- Concentration
- 1.22 mg/mL
- Storage
- Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
Submitted references A protocol for rapid pericyte differentiation of human induced pluripotent stem cells.
Aisenbrey EA, Torr E, Johnson H, Soref C, Daly W, Murphy WL
STAR protocols 2021 Mar 19;2(1):100261
STAR protocols 2021 Mar 19;2(1):100261
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunocytochemistry-Immunofluorescence analysis of TAGLN was performed in HeLa cells fixed in 4% paraformaldehyde at RT for 15 min. Green: TAGLN Polyclonal Antibody (Product # PA5-27463) diluted at 1:500. Red: alpha Tubulin, a cytoskeleton marker. Blue: Hoechst 33342 staining.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunocytochemistry-Immunofluorescence analysis of TAGLN was performed in HeLa cells fixed in 4% paraformaldehyde at RT for 15 min. Green: TAGLN Polyclonal Antibody (Product # PA5-27463) diluted at 1:500. Red: alpha Tubulin, a cytoskeleton marker. Blue: Hoechst 33342 staining.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunoprecipitation of Transgelin was performed in HeLa whole cell extracts using 5 µg of TAGLN Polyclonal Antibody (Product # PA5-27463). Samples were transferred to a membrane and probed with TAGLN Polyclonal Antibody as a primary antibody and an HRP-conjugated anti-Rabbit IgG was used as a secondary antibody.
