GTX629078

antibody from GeneTex

Targeting: TAGLN DKFZp686P11128, SM22, SMCC, TAGLN1, WS3-10

Western blot (Enhanced validation)

Immunohistochemistry (Data presented on provider website)

Antibody Data

Product number

GTX629078

Provider

GeneTex

Product name

Transgelin antibody [GT4511]

Antibody type

Monoclonal

Reactivity

Human, Mouse, Rat

Host

Mouse

Western blot

Enhanced validation

Submitted by

GeneTex (provider)

Enhanced method

Genetic validation

Main image

Experimental details

Non-transfected (¡V) and transfected (+) HeLa whole cell extracts (15 ?g) were separated by 12% SDS-PAGE, and the membrane was blotted with Transgelin antibody [GT4511] (GTX629078) diluted at 1:1000.

KD Reagent Type

siRNA

Downregulation

<50%

KD Reagent Prod page

KD Reagent Prod page

KD Reagent Provider

shRNA reagents was provided courtesy of cooperative labs.

KD Reagent Prod no

Proprietary.

Antibody Lot Number

41295

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Supportive validation

Submitted by

GeneTex (provider)

Main image

Experimental details

Transgelin antibody [GT4511] detects TAGLN protein by Western blot analysis.A. 30 ?g HeLa whole cell lysate/extract12 % SDS-PAGETransgelin antibody [GT4511] (GTX629078) dilution: 1:1000

Supportive validation

Submitted by

GeneTex (provider)

Main image

Experimental details

Non-transfected (¡V) and transfected (+) HeLa whole cell extracts (15 ?g) were separated by 12% SDS-PAGE, and the membrane was blotted with Transgelin antibody [GT4511] (GTX629078) diluted at 1:1000.

Supportive validation

Submitted by

GeneTex (provider)

Main image

Experimental details

Various whole cell extracts (30 ?g) were separated by 12% SDS-PAGE, and the membrane was blotted with Transgelin antibody [GT4511] (GTX629078) diluted at 1:1000. The HRP-conjugated anti-mouse IgG antibody (GTX213111-01) was used to detect the primary antibody.