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Western blotAntibody data
- Antibody Data
- Antigen structure
- References [1]
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- Validations
- Western blot [2]
- Immunocytochemistry [1]
- Immunohistochemistry [1]
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- Product number
- AF7886 - Provider product page
- Provider
- R&D Systems
- Product name
- Human/Mouse/Rat Transgelin/TAGLN Antibody
- Antibody type
- Polyclonal
- Description
- Immunogen affinity purified. Detects human, mouse, and rat Transgelin/TAGLN in Western blots. In direct ELISAs, less than 5% cross-reactivity with recombinant human (rh) TAGLN2 and rhTAGLN3 is observed.
- Reactivity
- Human, Mouse, Rat
- Host
- Sheep
- Conjugate
- Unconjugated
- Antigen sequence
Q01995- Isotype
- IgG
- Vial size
- 100 ug
- Concentration
- LYOPH
- Storage
- Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Submitted references LOXL2 Is Highly Expressed in Cancer-Associated Fibroblasts and Associates to Poor Colon Cancer Survival.
Torres S, Garcia-Palmero I, Herrera M, Bartolomé RA, Peña C, Fernandez-Aceñero MJ, Padilla G, Peláez-García A, Lopez-Lucendo M, Rodriguez-Merlo R, García de Herreros A, Bonilla F, Casal JI
Clinical cancer research : an official journal of the American Association for Cancer Research 2015 Nov 1;21(21):4892-902
Clinical cancer research : an official journal of the American Association for Cancer Research 2015 Nov 1;21(21):4892-902
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Supportive validation
- Submitted by
- R&D Systems (provider)
- Main image
- Experimental details
- Detection of Mouse and Rat Transgelin/TAGLN by Western Blot. Western blot shows lysates of mouse uterus tissue and rat uterus tissue. PVDF membrane was probed with 0.5 µg/mL of Sheep Anti-Human/Mouse/Rat Transgelin/TAGLN Antigen Affinity-purified Polyclonal Antibody (Catalog # AF7886) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). Specific bands were detected for Transgelin/TAGLN at approximately 18-22 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
- Submitted by
- R&D Systems (provider)
- Main image
- Experimental details
- Detection of Mouse Transgelin/TAGLN by Simple WesternTM. Simple Western lane view shows lysates of mouse uterus tissue, loaded at 0.2 mg/mL. A specific band was detected for Transgelin/TAGLN at approximately 29 kDa (as indicated) using 25 µg/mL of Sheep Anti-Human/Mouse/Rat Transgelin/TAGLN Antigen Affinity-purified Polyclonal Antibody (Catalog # AF7886) followed by 1:50 dilution of HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). This experiment was conducted under reducing conditions and using the 12-230 kDa separation system.
Supportive validation
- Submitted by
- R&D Systems (provider)
- Main image
- Experimental details
- Transgelin/TAGLN in MCF 10A Human Cell Line. Transgelin/TAGLN was detected in immersion fixed MCF 10A human breast epithelial cell line using Sheep Anti-Human/Mouse/Rat Transgelin/TAGLN Antigen Affinity-purified Polyclonal Antibody (Catalog # AF7886) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Sheep IgG Secondary Antibody (red; Catalog # NL010) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.
Supportive validation
- Submitted by
- R&D Systems (provider)
- Main image
- Experimental details
- Transgelin/TAGLN in Human Liver Cancer Tissue. Transgelin/TAGLN was detected in immersion fixed paraffin-embedded sections of human liver cancer tissue using Sheep Anti-Human/Mouse/Rat Transgelin/TAGLN Antigen Affinity-purified Polyclonal Antibody (Catalog # AF7886) at 1.7 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Sheep HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS019) and counterstained with hematoxylin (blue). Specific staining was localized to connective tissue. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.
