Explore
Validate
Learn
Flow cytometryAntibody data
- Antibody Data
- Antigen structure
- References [8]
- Comments [0]
- Validations
- Flow cytometry [1]
- Other assay [1]
Submit
Validation data
Reference
Comment
Report error
- Product number
- 14-2059-82 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- CD205 Monoclonal Antibody (MG38), eBioscience™
- Antibody type
- Monoclonal
- Antigen
- Other
- Description
- Description: The MG38 monoclonal antibody reacts with human CD205, also known as DEC-205, an integral membrane protein homologous to the macrophage mannose receptor. CD205 is an endocytic dendritic receptor that mediates efficient processing and presentation of antigens on MHC class II productions in vivo. CD205 is highly expressed by dendritic cells (DCs) within the T cell areas of lymphoid tissues, particularly on CD8^+ DCs that are believed to play a role in cross-priming. Expression of CD205 is detected with this antibody at high intensity on in vitro derived mature human DCs (mDC) and at intermediate intensity on immature human DCs (iDC). All leukocytes from peripheral blood show a degree of staining with this mAb that can be minimized by using very low concentrations of MG38.
- Antibody clone number
- MG38
- Concentration
- 0.5 mg/mL
Submitted references Dendritic cells and vaccine design for sexually-transmitted diseases.
DEC-205 mediates antigen uptake and presentation by both resting and activated human plasmacytoid dendritic cells.
Dendritic cell interaction with Candida albicans critically depends on N-linked mannan.
Dendritic cells are abundant in non-lesional gray matter in multiple sclerosis.
Expression of human DEC-205 (CD205) multilectin receptor on leukocytes.
Efficient targeting of protein antigen to the dendritic cell receptor DEC-205 in the steady state leads to antigen presentation on major histocompatibility complex class I products and peripheral CD8+ T cell tolerance.
Dendritic cells induce peripheral T cell unresponsiveness under steady state conditions in vivo.
A monoclonal antibody to the DEC-205 endocytosis receptor on human dendritic cells.
Duluc D, Gannevat J, Joo H, Ni L, Upchurch K, Boreham M, Carley M, Stecher J, Zurawski G, Oh S
Microbial pathogenesis 2013 May;58:35-44
Microbial pathogenesis 2013 May;58:35-44
DEC-205 mediates antigen uptake and presentation by both resting and activated human plasmacytoid dendritic cells.
Tel J, Benitez-Ribas D, Hoosemans S, Cambi A, Adema GJ, Figdor CG, Tacken PJ, de Vries IJ
European journal of immunology 2011 Apr;41(4):1014-23
European journal of immunology 2011 Apr;41(4):1014-23
Dendritic cell interaction with Candida albicans critically depends on N-linked mannan.
Cambi A, Netea MG, Mora-Montes HM, Gow NA, Hato SV, Lowman DW, Kullberg BJ, Torensma R, Williams DL, Figdor CG
The Journal of biological chemistry 2008 Jul 18;283(29):20590-9
The Journal of biological chemistry 2008 Jul 18;283(29):20590-9
Dendritic cells are abundant in non-lesional gray matter in multiple sclerosis.
Cudrici C, Ito T, Zafranskaia E, Niculescu F, Mullen KM, Vlaicu S, Judge SI, Calabresi PA, Rus H
Experimental and molecular pathology 2007 Oct;83(2):198-206
Experimental and molecular pathology 2007 Oct;83(2):198-206
Expression of human DEC-205 (CD205) multilectin receptor on leukocytes.
Kato M, McDonald KJ, Khan S, Ross IL, Vuckovic S, Chen K, Munster D, MacDonald KP, Hart DN
International immunology 2006 Jun;18(6):857-69
International immunology 2006 Jun;18(6):857-69
Efficient targeting of protein antigen to the dendritic cell receptor DEC-205 in the steady state leads to antigen presentation on major histocompatibility complex class I products and peripheral CD8+ T cell tolerance.
Bonifaz L, Bonnyay D, Mahnke K, Rivera M, Nussenzweig MC, Steinman RM
The Journal of experimental medicine 2002 Dec 16;196(12):1627-38
The Journal of experimental medicine 2002 Dec 16;196(12):1627-38
Dendritic cells induce peripheral T cell unresponsiveness under steady state conditions in vivo.
Hawiger D, Inaba K, Dorsett Y, Guo M, Mahnke K, Rivera M, Ravetch JV, Steinman RM, Nussenzweig MC
The Journal of experimental medicine 2001 Sep 17;194(6):769-79
The Journal of experimental medicine 2001 Sep 17;194(6):769-79
A monoclonal antibody to the DEC-205 endocytosis receptor on human dendritic cells.
Guo M, Gong S, Maric S, Misulovin Z, Pack M, Mahnke K, Nussenzweig MC, Steinman RM
Human immunology 2000 Aug;61(8):729-38
Human immunology 2000 Aug;61(8):729-38
No comments: Submit comment
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Staining of normal human peripheral blood cells (left) and mature dendritic cells (right) with Mouse IgG2b kappa Isotype Control Purified (Product # 14-4732-82) (open histogram) or 0.015 µg of Anti-Human CD205 Purified (filled histogram) followed by Anti-Mouse IgG Biotin (Product # 13-4013-85) and Streptavidin PE (Product # 12-4317-87). Total viable cells were used for analysis.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- NULL
