14-2059-37

antibody from Invitrogen Antibodies
Targeting: LY75 CD205, CLEC13B, DEC-205
Provider product page for 14-2059-37
Immunoprecipitation
Immunohistochemistry
Flow cytometry
Other assay

Antibody data

Product number
14-2059-37 - Provider product page
Provider
Invitrogen Antibodies
Product name
CD205 Monoclonal Antibody (MG38), eBioscience™
Antibody type
Monoclonal
Antigen
Other
Description
Description: The MG38 monoclonal antibody reacts with human CD205, also known as DEC-205, an integral membrane protein homologous to the macrophage mannose receptor. CD205 is an endocytic dendritic receptor that mediates efficient processing and presentation of antigens on MHC class II productions in vivo. CD205 is highly expressed by dendritic cells (DCs) within the T cell areas of lymphoid tissues, particularly on CD8^+ DCs that are believed to play a role in cross-priming. Expression of CD205 is detected with this antibody at high intensity on in vitro derived mature human DCs (mDC) and at intermediate intensity on immature human DCs (iDC). All leukocytes from peripheral blood show a degree of staining with this mAb that can be minimized by using very low concentrations of MG38. Applications Reported: The MG38 antibody has been reported for use in flow cytometric analysis, immunoprecipitation, and immunohistochemical staining of frozen tissue sections. Applications Tested: This MG38 antibody has been tested by flow cytometric analysis of in vitro derived human dendritic cells and peripheral blood cells. This can be used at less than or equal to 0.03 µg per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest. Purity: Greater than 90%, as determined by SDS-PAGE. Aggregation: Less than 10%, as determined by HPLC. Filtration: 0.2 µm post-manufacturing filtered.
Reactivity
Human
Host
Mouse
Isotype
IgG
Antibody clone number
MG38
Vial size
2 mg
Concentration
0.5 mg/mL
Storage
4° C
LY75 protein structure - 14-2059-37 shown in red.