Antibody data
- Antibody Data
- Antigen structure
- References [4]
- Comments [0]
- Validations
- Other assay [1]
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- Product number
- MA1-43012 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- Factor IX Monoclonal Antibody (5041)
- Antibody type
- Monoclonal
- Antigen
- Other
- Description
- MA1-43012 detects Factor IX in human samples, human factor Ixa and the heavy chain of human factor IX and IXa. MA1-43012 has successfully been used in radioimmune assays, ELISA, Western blot and immunohistochemistry applications. MA1-43012 was derived from human factor IX.
- Reactivity
- Human
- Host
- Mouse
- Isotype
- IgG
- Antibody clone number
- 5041
- Vial size
- 100 µg
- Concentration
- 9.5 mg/mL
- Storage
- -20° C, Avoid Freeze/Thaw Cycles
Submitted references Ex vivo editing of human hematopoietic stem cells for erythroid expression of therapeutic proteins.
Preclinical Development of an AAV8-hUGT1A1 Vector for the Treatment of Crigler-Najjar Syndrome.
Antigen-selective modulation of AAV immunogenicity with tolerogenic rapamycin nanoparticles enables successful vector re-administration.
Influence of Pre-existing Anti-capsid Neutralizing and Binding Antibodies on AAV Vector Transduction.
Pavani G, Laurent M, Fabiano A, Cantelli E, Sakkal A, Corre G, Lenting PJ, Concordet JP, Toueille M, Miccio A, Amendola M
Nature communications 2020 Jul 29;11(1):3778
Nature communications 2020 Jul 29;11(1):3778
Preclinical Development of an AAV8-hUGT1A1 Vector for the Treatment of Crigler-Najjar Syndrome.
Collaud F, Bortolussi G, Guianvarc'h L, Aronson SJ, Bordet T, Veron P, Charles S, Vidal P, Sola MS, Rundwasser S, Dufour DG, Lacoste F, Luc C, Wittenberghe LV, Martin S, Le Bec C, Bosma PJ, Muro AF, Ronzitti G, Hebben M, Mingozzi F
Molecular therapy. Methods & clinical development 2019 Mar 15;12:157-174
Molecular therapy. Methods & clinical development 2019 Mar 15;12:157-174
Antigen-selective modulation of AAV immunogenicity with tolerogenic rapamycin nanoparticles enables successful vector re-administration.
Meliani A, Boisgerault F, Hardet R, Marmier S, Collaud F, Ronzitti G, Leborgne C, Costa Verdera H, Simon Sola M, Charles S, Vignaud A, van Wittenberghe L, Manni G, Christophe O, Fallarino F, Roy C, Michaud A, Ilyinskii P, Kishimoto TK, Mingozzi F
Nature communications 2018 Oct 5;9(1):4098
Nature communications 2018 Oct 5;9(1):4098
Influence of Pre-existing Anti-capsid Neutralizing and Binding Antibodies on AAV Vector Transduction.
Fitzpatrick Z, Leborgne C, Barbon E, Masat E, Ronzitti G, van Wittenberghe L, Vignaud A, Collaud F, Charles S, Simon Sola M, Jouen F, Boyer O, Mingozzi F
Molecular therapy. Methods & clinical development 2018 Jun 15;9:119-129
Molecular therapy. Methods & clinical development 2018 Jun 15;9:119-129
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Supportive validation
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- Invitrogen Antibodies (provider)
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- Experimental details
- Fig. 3 F9 KI into the alpha-globin locus results in expression and secretion of functional enzyme. a AAV6 donor used for KI experiments of FIX Padua. b FIX KI efficiency in HUDEP-2 cells was measured by flow cytometry (light green) or ddPCR specific for on-target integration (dark green) before and after sorting ( n = 1). c Quantification of FIX mRNA in KI HUDEP-2 upon differentiation (mean +- SD, n = 2 undifferentiated, n = 3 differentiated). d Quantification of FIX secretion in medium of HUDEP-2 clones ( n = 28) with monoallelic or biallelic KI (ELISA), as detected by on target ddPCR analysis (AAV-genome junction amplification). Lines represent median. e KI efficiency in HSPCs at day 9 of erythroid differentiation. Lines represent mean ( n = 4). f , g FIX expression during HSPC differentiation at RNA ( f , qPCR; n = 2 day 9; n = 4 day 12) and protein level ( g , ELISA on supernatants, n = 3 day 7; n = 4 day 9 and 12; 3 donors). Bars represent mean +- SD. h Comparison of FIX antigen (ELISA) and activity (aPTT) in supernatants of KI HSPCs (mean; n = 2). i , j Comparison of FIX RNA at day 9 and 12 of erythroid differentiation ( i ) and protein ( j ) in KI HSPCs (AAV + RNP) vs HSPCs transduced with an erythroid-specific lentiviral vector (LvEry FIX). Bars represent mean (** p = 0.003 t -test Holm-Sidak correction for RNA at day 12; p = 0.08 for protein, n = 2). k Integration pattern in single BFU-E (2 donors): no integration (0), monoallelic (1) and biallelic KI (2). Source dat