ABIN458123

antibody from antibodies-online

Targeting: F9 FIX

No application information provided.

Antibody Data

Product number

ABIN458123

Provider

antibodies-online

Proper citation

Antibodies-Online Cat#ABIN458123, RRID:AB_10781248

Product name

anti-Coagulation Factor IX (F9) antibody

Antibody type

Polyclonal

Antigen

Factor IX is a vitamin K-dependent glycoprotein, MW 55,400, and it consists of a single polypeptide chain. It is synthesized in the liver, stable for several days in fresh blood and for many months in fresh frozen plasma. It can be prepared as a stable concentrate. Its physicochemical properties are similar to those of several other clotting factors, which makes separation particularly difficult. Factor IX is converted to FIXa by FXIa in the presence of calcium ions. Platelets possess FXI-like activity, bypassing FXII an XI. FIXa has a molecular weight of 46,500 and is composed of two chains linked by disulphide bonds. For immunization purposes, FIX can be obtained n highly purified form from fresh plasma concentrates. After coagulation, FIX antigen is also present in serum. Abnormal molecular variants are defective in proteolytic activation and serine protease activity, while calcium binding is also impaired. Freund€™s complete adjuvant is used in the first step of the immunization procedure.

Reactivity

Human

Host

Rabbit

Vial size

1 ml

Concentration

Total protein and IgG concentrations in the antiserum are comparable to those of pooled normal rabbit serum. No foreign proteins added. Antibody titre: Precipitin titre not less than 1:32 when tested against appropriate concentrations of the antigen in ag

Storage

Lyophilised at +4°C (10 years), reconstituted at or below -20°C (3-5 years), reconstituted at +4°C at least 7 days