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Western blotAntibody data
- Antibody Data
- Antigen structure
- References [1]
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- Validations
- Western blot [1]
- Immunocytochemistry [2]
- Immunohistochemistry [4]
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- Product number
- PA5-22304 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- Factor IX Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Recombinant protein fragment
- Description
- Predicted reactivity: Mouse (80%), Rat (82%), Dog (87%), Cat (84%), Pig (87%), Rabbit (82%), Rhesus Monkey (96%), Chimpanzee (99%), Bovine (82%).
- Concentration
- 1.51 mg/mL
Submitted references Generation of an mESC model with a human hemophilia B nonsense mutation via CRISPR/Cas9 technology.
Ma Y, Sun W, Zhao L, Yao M, Wu C, Su P, Yang L, Wang G
Stem cell research & therapy 2022 Jul 26;13(1):353
Stem cell research & therapy 2022 Jul 26;13(1):353
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of Factor IX using 30 µg of 293T lysate. Samples were loaded onto a 10% SDS-PAGE gel and probed with a Factor IX polyclonal antibody (Product # PA5-22304) at a dilution of 1:1000.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunocytochemistry-Immunofluorescence analysis of Factor IX was performed in HepG2 cells fixed in 4% paraformaldehyde at RT for 15 min. Green: Factor IX Polyclonal Antibody (Product # PA5 22304) diluted at 1:500. Blue: Hoechst 33342 staining.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunocytochemistry-Immunofluorescence analysis of Factor IX was performed in HepG2 cells fixed in 4% paraformaldehyde at RT for 15 min. Green: Factor IX Polyclonal Antibody (Product # PA5 22304) diluted at 1:500. Blue: Hoechst 33342 staining.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemistry (Paraffin) analysis of Factor IX was performed in paraffin-embedded human colon cancer tissue using Factor IX Polyclonal Antibody (Product # PA5-22304) at a dilution of 1:250.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemistry (Paraffin) analysis of Factor IX was performed in paraffin-embedded rat liver tissue using Factor IX Polyclonal Antibody (Product # PA5-22304) at a dilution of 1:500.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemistry (Paraffin) analysis of Factor IX was performed in paraffin-embedded mouse liver tissue using Factor IX Polyclonal Antibody (Product # PA5-22304) at a dilution of 1:500. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemistry (Paraffin) analysis of Factor IX was performed in paraffin-embedded mouse pancreas tissue using Factor IX Polyclonal Antibody (Product # PA5-22304) at a dilution of 1:500.
