LS-C98844

antibody from LSBio

Targeting: BCL2L2 BCL-W, KIAA0271, PPP1R51

Western blot (Supportive data in Antibodypedia)

Immunohistochemistry (Supportive data in Antibodypedia)

Antibody Data

Product number

LS-C98844

Provider

LSBio

Proper citation

LifeSpan Cat#LS-C98844, RRID:AB_2290501

Product name

BCL2L2 / Bcl-w Antibody (aa24-59) LS-C98844

Antibody type

Polyclonal

Description

Ammonium sulfate precipitation

Reactivity

Human, Mouse

Host

Rabbit

Storage

Maintain refrigerated at 2°C to 8°C for up to 6 months. For long term storage store at -20°C.

Western blot

Supportive validation

Submitted by

LSBio (provider)

Enhanced method

Genetic validation

Main image

Experimental details

Western blot of anti-Bcl-w BH3 domain antibody in HL-60 cell lysate. Bcl-w BH3 domain (arrow) was detected using purified antibody. Secondary HRP-anti-rabbit was used for signal visualization with chemiluminescence.

Supportive validation

Submitted by

LSBio (provider)

Enhanced method

Genetic validation

Main image

Experimental details

Western blot of Bcl-w3 (arrow) using rabbit polyclonal Bcl-w BH3 Domain Antibody. 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected with the BCL2L2 gene (Lane 2) (Origene Technologies).

Supportive validation

Submitted by

LSBio (provider)

Enhanced method

Genetic validation

Main image

Experimental details

BCLW Antibody (BH3) (RB00972) western blot of mouse NIH-3T3 cell line lysates (35 ug/lane). The BCLW antibody detected the BCLW protein (arrow).

Immunohistochemistry

Supportive validation

Submitted by

LSBio (provider)

Enhanced method

Genetic validation

Main image

Experimental details

Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. BC = breast carcinoma; HC = hepatocarcinoma.

Supportive validation

Submitted by

LSBio (provider)

Enhanced method

Genetic validation

Main image

Experimental details

Formalin-fixed and paraffin-embedded human breast carcinoma tissue reacted with Bcl-w BH3 Domain Antibody , which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.