Antibody data
- Antibody Data
- Antigen structure
- References [1]
- Comments [0]
- Validations
- Western blot [2]
- Immunohistochemistry [1]
- Other assay [1]
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Validation data
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- Product number
- PA5-51430 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- Bcl-W Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Recombinant full-length protein
- Description
- Immunogen sequence: MSIEEKMEAD ARSIYVGNVD YGATAEELEA HFHGCGSVNR VTILCDKFSG HPKGFAYIEF SDKESVRTSL ALDESLFRGR QIKVIPKRTN RPGISTTDRG FPRARYRART TNYNSSRSRF YSGFNSRPRG RVYRGRARAT SWY Highest antigen sequence identity to the following orthologs: Mouse - 98%, Rat - 99%.
- Reactivity
- Human, Mouse
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 100 µL
- Concentration
- 0.1 mg/mL
- Storage
- Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
Submitted references An Anti-PSMA Immunotoxin Reduces Mcl-1 and Bcl2A1 and Specifically Induces in Combination with the BAD-Like BH3 Mimetic ABT-737 Apoptosis in Prostate Cancer Cells.
Masilamani AP, Dettmer-Monaco V, Monaco G, Cathomen T, Kuckuck I, Schultze-Seemann S, Huber N, Wolf P
Cancers 2020 Jun 22;12(6)
Cancers 2020 Jun 22;12(6)
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of Bcl-W in Lane 1: Marker (kDa) 250, 130, 100, 70, 55, 35, 25, 15, 10; Lane 2: Human cerebral cortex tissue. Samples were probed using a Bcl-W Polyclonal Antibody (Product # PA5-51430).
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of Bcl-W in Lane 1: Marker (kDa) 250, 130, 100, 70, 55, 35, 25, 15, 10; Lane 2: Mouse cerebral cortex tissue. Samples were probed using a Bcl-W Polyclonal Antibody (Product # PA5-51430).
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical staining of Bcl-W in human testis using a Bcl-W Polyclonal Antibody (Product # PA5-51430) shows strong nuclear positivity in cells in seminiferous ducts.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Figure 3 The immunotoxin hD7-1(VL-VH)-PE40 induces apoptosis in PSMA-positive cells by reducing the expression of Mcl-1 and Bcl2A1. PC cells were incubated for different time points with 1 nM immunotoxin. After cell lysis, expression of the anti-apoptotic Bcl-2 proteins Bcl-2, Bcl-xl, Mcl-1, Bcl-w, and Bcl2A1, the pro-apoptotic Bcl-2 proteins Bax and Bak, as well as the activation of caspase-3, and -8, and the cleavage of PARP were determined by Western blot. ss-actin was detected as loading control.