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Western blotAntibody data
- Antibody Data
- Antigen structure
- References [3]
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- Validations
- Western blot [1]
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- Product number
- AF824 - Provider product page
- Provider
- R&D Systems
- Product name
- Human/Mouse Bcl-w Antibody
- Antibody type
- Polyclonal
- Description
- Antigen Affinity-purified. Detects human and mouse Bcl-w in Western blots.
- Reactivity
- Human, Mouse
- Host
- Rabbit
- Conjugate
- Unconjugated
- Antigen sequence
Q92843- Isotype
- IgG
- Vial size
- 120 ug
- Concentration
- LYOPH
- Storage
- Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Submitted references Sensitivity to splicing modulation of BCL2 family genes defines cancer therapeutic strategies for splicing modulators.
Methamphetamine causes differential regulation of pro-death and anti-death Bcl-2 genes in the mouse neocortex.
Methamphetamine causes differential regulation of pro-death and anti-death Bcl-2 genes in the mouse neocortex.
Aird D, Teng T, Huang CL, Pazolli E, Banka D, Cheung-Ong K, Eifert C, Furman C, Wu ZJ, Seiler M, Buonamici S, Fekkes P, Karr C, Palacino J, Park E, Smith PG, Yu L, Mizui Y, Warmuth M, Chicas A, Corson L, Zhu P
Nature communications 2019 Jan 11;10(1):137
Nature communications 2019 Jan 11;10(1):137
Methamphetamine causes differential regulation of pro-death and anti-death Bcl-2 genes in the mouse neocortex.
Jayanthi S, Deng X, Bordelon M, McCoy MT, Cadet JL
FASEB journal : official publication of the Federation of American Societies for Experimental Biology 2001 Aug;15(10):1745-52
FASEB journal : official publication of the Federation of American Societies for Experimental Biology 2001 Aug;15(10):1745-52
Methamphetamine causes differential regulation of pro-death and anti-death Bcl-2 genes in the mouse neocortex.
Jayanthi S, Deng X, Bordelon M, McCoy MT, Cadet JL
FASEB journal : official publication of the Federation of American Societies for Experimental Biology 2001 Aug;15(10):1745-52
FASEB journal : official publication of the Federation of American Societies for Experimental Biology 2001 Aug;15(10):1745-52
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Supportive validation
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- R&D Systems (provider)
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- Experimental details
- Detection of Human and Mouse Bcl-w by Western Blot. Western blot shows lysates of WS-1 human fetal skin fibroblast cell line and CTLL-2 mouse cytotoxic T cell line. PVDF membrane was probed with 1.2 µg/mL Rabbit Anti-Human/Mouse Bcl-w Antigen Affinity-purified Polyclonal Antibody (Catalog # AF824) followed by HRP-conjugated Anti-Rabbit IgG Secondary Antibody (Catalog # HAF008). For additional reference, Recombinant Human Bcl-w Minus C-Terminus (Catalog # 824-BW) (1 ng) was included. A specific band for Bcl-w was detected at approximately 19 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 4.
