MAB8241

antibody from R&D Systems

Targeting: BCL2L2 BCL-W, KIAA0271, PPP1R51

Blocking/Neutralizing (Supportive data in Antibodypedia)

Antibody Data

Product number

MAB8241

Provider

R&D Systems

Product name

Human/Mouse Bcl-w Antibody

Antibody type

Monoclonal

Description

Protein A or G purified from hybridoma culture supernatant. Detects human and mouse Bcl-w. Neutralizes recombinant human and mouse Bcl-w, but not recombinant human Bcl-2 or Bcl-x.

Reactivity

Human, Mouse

Host

Mouse

Conjugate

Unconjugated

Antigen sequence

Q92843


Isotype

IgG

Antibody clone number

90336

Vial size

250 ug

Concentration

LYOPH

Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Blocking/Neutralizing

Supportive validation

Submitted by

R&D Systems (provider)

Main image

Experimental details

Bcl-w Inhibition of BID-induced Release of Cytochrome c and Neutralization by BCL-w Antibody. Cytochrome c released from enriched mouse liver mitochondria solubilized with Triton^® X-100 was quantified with the Rat/Mouse Cytochrome c ELISA (Catalog # MCTC0).Untreated: Release of Cytochrome c from untreated mitochondria BID: Recombinant Human BID (Caspase-8-cleaved) (Catalog # 882-B8) induced release of Cytochrome c from mitochondria BID + Bcl-w: Recombinant Human Bcl-w (Catalog # 824-BW) inhibition of Recombinant Human BID (Caspase-8-cleaved) (Catalog # 882-B8) induced release of Cytochrome c from mitochondria BID + Bcl-w + MAB8241: Mouse Anti-Human/Mouse Bcl-w Monoclonal Antibody (Catalog # MAB8241) neutralized Recombinant Human Bcl-w (Catalog # 824-BW) inhibition of Recombinant Human BID (Caspase-8-cleaved) (Catalog # 882-B8) induced release of Cytochrome c from mitochondria