Antibody data
- Antibody Data
- Antigen structure
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- Comments [0]
- Validations
- Blocking/Neutralizing [1]
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Validation data
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- Product number
- MAB8241 - Provider product page
- Provider
- R&D Systems
- Product name
- Human/Mouse Bcl-w Antibody
- Antibody type
- Monoclonal
- Description
- Protein A or G purified from hybridoma culture supernatant. Detects human and mouse Bcl-w. Neutralizes recombinant human and mouse Bcl-w, but not recombinant human Bcl-2 or Bcl-x.
- Reactivity
- Human, Mouse
- Host
- Mouse
- Conjugate
- Unconjugated
- Antigen sequence
Q92843
- Isotype
- IgG
- Antibody clone number
- 90336
- Vial size
- 250 ug
- Concentration
- LYOPH
- Storage
- Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 6 months, -20 to -70 °C under sterile conditions after reconstitution.
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Supportive validation
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- R&D Systems (provider)
- Main image
- Experimental details
- Bcl-w Inhibition of BID-induced Release of Cytochrome c and Neutralization by BCL-w Antibody. Cytochrome c released from enriched mouse liver mitochondria solubilized with Triton® X-100 was quantified with the Rat/Mouse Cytochrome c ELISA (Catalog # MCTC0).Untreated: Release of Cytochrome c from untreated mitochondria BID: Recombinant Human BID (Caspase-8-cleaved) (Catalog # 882-B8) induced release of Cytochrome c from mitochondria BID + Bcl-w: Recombinant Human Bcl-w (Catalog # 824-BW) inhibition of Recombinant Human BID (Caspase-8-cleaved) (Catalog # 882-B8) induced release of Cytochrome c from mitochondria BID + Bcl-w + MAB8241: Mouse Anti-Human/Mouse Bcl-w Monoclonal Antibody (Catalog # MAB8241) neutralized Recombinant Human Bcl-w (Catalog # 824-BW) inhibition of Recombinant Human BID (Caspase-8-cleaved) (Catalog # 882-B8) induced release of Cytochrome c from mitochondria