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- References [18]
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- Validations
- Western blot [1]
- Immunocytochemistry [1]
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- Product number
- 10362-1-AP - Provider product page
- Provider
- Proteintech Group
- Proper citation
- Proteintech Cat#10362-1-AP, RRID:AB_2080345
- Product name
- Chk1 antibody
- Antibody type
- Polyclonal
- Description
- KD/KO validated Chk1 antibody (Cat. #10362-1-AP) is a rabbit polyclonal antibody that shows reactivity with human, mouse, rat and has been validated for the following applications: FC, IF, IHC, WB,ELISA.
- Reactivity
- Human, Mouse, Rat
- Host
- Rabbit
- Conjugate
- Unconjugated
- Isotype
- IgG
- Vial size
- 20ul, 150ul
Submitted references Role of inhibiting Chk1-p53 pathway in hepatotoxicity caused by chronic arsenic exposure from coal-burning.
Novel SAHA‑bendamustine hybrid NL‑101 in combination with daunorubicin synergistically suppresses acute myeloid leukemia.
HOTAIR promotes paclitaxel resistance by regulating CHEK1 in ovarian cancer.
Histone H3K27 methylation modulates the dynamics of FANCD2 on chromatin to facilitate NHEJ and genome stability.
HBV infection potentiates resistance to S-phase arrest-inducing chemotherapeutics by inhibiting CHK2 pathway in diffuse large B-cell lymphoma.
High PARP-1 expression predicts poor survival in acute myeloid leukemia and PARP-1 inhibitor and SAHA-bendamustine hybrid inhibitor combination treatment synergistically enhances anti-tumor effects.
PFKFB3 blockade inhibits hepatocellular carcinoma growth by impairing DNA repair through AKT.
Anthocyanins from Black Chokeberry (Aroniamelanocarpa Elliot) Delayed Aging-Related Degenerative Changes of Brain.
Anthocyanins Delay Ageing-Related Degenerative Changes in the Liver.
Downregulation of Ubiquitin-conjugating Enzyme UBE2D3 Promotes Telomere Maintenance and Radioresistance of Eca-109 Human Esophageal Carcinoma Cells.
UBE2D3 gene overexpression increases radiosensitivity of EC109 esophageal cancer cells in vitro and in vivo.
Synergistic anti-leukemic interactions between panobinostat and MK-1775 in acute myeloid leukemia ex vivo.
Polychlorinated Biphenyl Quinone Metabolite Promotes p53-Dependent DNA Damage Checkpoint Activation, S-Phase Cycle Arrest and Extrinsic Apoptosis in Human Liver Hepatocellular Carcinoma HepG2 Cells.
Human Tra2 proteins jointly control a CHEK1 splicing switch among alternative and constitutive target exons.
Probing the cell death signaling pathway of HepG2 cell line induced by copper-1,10-phenanthroline complex.
MAPK signaling mediates sinomenine hydrochloride-induced human breast cancer cell death via both reactive oxygen species-dependent and -independent pathways: an in vitro and in vivo study.
PPE38 of Mycobacterium marinum triggers the cross-talk of multiple pathways involved in the host response, as revealed by subcellular quantitative proteomics.
Checkpoint kinase 1 (Chk1)-short is a splice variant and endogenous inhibitor of Chk1 that regulates cell cycle and DNA damage checkpoints.
Yang Y, Liu C, Xie T, Wang D, Chen X, Ma L, Zhang A
Human & experimental toxicology 2021 Jul;40(7):1141-1152
Human & experimental toxicology 2021 Jul;40(7):1141-1152
Novel SAHA‑bendamustine hybrid NL‑101 in combination with daunorubicin synergistically suppresses acute myeloid leukemia.
Jin J, Li X, Guo W, Li F, Huang J, Huang X, Pan J, Huang S, Ye W, Ling Q, Jin J
Oncology reports 2020 Jul;44(1):273-282
Oncology reports 2020 Jul;44(1):273-282
HOTAIR promotes paclitaxel resistance by regulating CHEK1 in ovarian cancer.
Jiang J, Wang S, Wang Z, Cai J, Han L, Xie L, Han Q, Wang W, Zhang Y, He X, Yang C
Cancer chemotherapy and pharmacology 2020 Aug;86(2):295-305
Cancer chemotherapy and pharmacology 2020 Aug;86(2):295-305
Histone H3K27 methylation modulates the dynamics of FANCD2 on chromatin to facilitate NHEJ and genome stability.
Zhang Y, Chang JF, Sun J, Chen L, Yang XM, Tang HY, Jing YY, Kang X, He ZM, Wu JY, Wei HM, Wang DL, Xu RG, Zhu RB, Shen Y, Zeng SY, Wang C, Liu KN, Zhang Y, Mao ZY, Jiang CZ, Sun FL
Journal of cell science 2018 Jun 21;131(12)
Journal of cell science 2018 Jun 21;131(12)
HBV infection potentiates resistance to S-phase arrest-inducing chemotherapeutics by inhibiting CHK2 pathway in diffuse large B-cell lymphoma.
Zhao X, Guo X, Xing L, Yue W, Yin H, He M, Wang J, Yang J, Chen J
Cell death & disease 2018 Jan 19;9(2):61
Cell death & disease 2018 Jan 19;9(2):61
High PARP-1 expression predicts poor survival in acute myeloid leukemia and PARP-1 inhibitor and SAHA-bendamustine hybrid inhibitor combination treatment synergistically enhances anti-tumor effects.
Li X, Li C, Jin J, Wang J, Huang J, Ma Z, Huang X, He X, Zhou Y, Xu Y, Yu M, Huang S, Yan X, Li F, Pan J, Wang Y, Yu Y, Jin J
EBioMedicine 2018 Dec;38:47-56
EBioMedicine 2018 Dec;38:47-56
PFKFB3 blockade inhibits hepatocellular carcinoma growth by impairing DNA repair through AKT.
Shi WK, Zhu XD, Wang CH, Zhang YY, Cai H, Li XL, Cao MQ, Zhang SZ, Li KS, Sun HC
Cell death & disease 2018 Apr 1;9(4):428
Cell death & disease 2018 Apr 1;9(4):428
Anthocyanins from Black Chokeberry (Aroniamelanocarpa Elliot) Delayed Aging-Related Degenerative Changes of Brain.
Wei J, Zhang G, Zhang X, Xu D, Gao J, Fan J, Zhou Z
Journal of agricultural and food chemistry 2017 Jul 26;65(29):5973-5984
Journal of agricultural and food chemistry 2017 Jul 26;65(29):5973-5984
Anthocyanins Delay Ageing-Related Degenerative Changes in the Liver.
Wei J, Zhang G, Zhang X, Xu D, Gao J, Fan J
Plant foods for human nutrition (Dordrecht, Netherlands) 2017 Dec;72(4):425-431
Plant foods for human nutrition (Dordrecht, Netherlands) 2017 Dec;72(4):425-431
Downregulation of Ubiquitin-conjugating Enzyme UBE2D3 Promotes Telomere Maintenance and Radioresistance of Eca-109 Human Esophageal Carcinoma Cells.
Yang H, Wu L, Ke S, Wang W, Yang L, Gao X, Fang H, Yu H, Zhong Y, Xie C, Zhou F, Zhou Y
Journal of Cancer 2016;7(9):1152-62
Journal of Cancer 2016;7(9):1152-62
UBE2D3 gene overexpression increases radiosensitivity of EC109 esophageal cancer cells in vitro and in vivo.
Gao X, Wang W, Yang H, Wu L, He Z, Zhou S, Zhao H, Fu Z, Zhou F, Zhou Y
Oncotarget 2016 May 31;7(22):32543-53
Oncotarget 2016 May 31;7(22):32543-53
Synergistic anti-leukemic interactions between panobinostat and MK-1775 in acute myeloid leukemia ex vivo.
Qi W, Zhang W, Edwards H, Chu R, Madlambayan GJ, Taub JW, Wang Z, Wang Y, Li C, Lin H, Ge Y
Cancer biology & therapy 2015;16(12):1784-93
Cancer biology & therapy 2015;16(12):1784-93
Polychlorinated Biphenyl Quinone Metabolite Promotes p53-Dependent DNA Damage Checkpoint Activation, S-Phase Cycle Arrest and Extrinsic Apoptosis in Human Liver Hepatocellular Carcinoma HepG2 Cells.
Song X, Li L, Shi Q, Lehmler HJ, Fu J, Su C, Xia X, Song E, Song Y
Chemical research in toxicology 2015 Nov 16;28(11):2160-9
Chemical research in toxicology 2015 Nov 16;28(11):2160-9
Human Tra2 proteins jointly control a CHEK1 splicing switch among alternative and constitutive target exons.
Best A, James K, Dalgliesh C, Hong E, Kheirolahi-Kouhestani M, Curk T, Xu Y, Danilenko M, Hussain R, Keavney B, Wipat A, Klinck R, Cowell IG, Cheong Lee K, Austin CA, Venables JP, Chabot B, Santibanez Koref M, Tyson-Capper A, Elliott DJ
Nature communications 2014 Sep 11;5:4760
Nature communications 2014 Sep 11;5:4760
Probing the cell death signaling pathway of HepG2 cell line induced by copper-1,10-phenanthroline complex.
Wu J, Chen W, Yin Y, Zheng Z, Zou G
Biometals : an international journal on the role of metal ions in biology, biochemistry, and medicine 2014 Jun;27(3):445-58
Biometals : an international journal on the role of metal ions in biology, biochemistry, and medicine 2014 Jun;27(3):445-58
MAPK signaling mediates sinomenine hydrochloride-induced human breast cancer cell death via both reactive oxygen species-dependent and -independent pathways: an in vitro and in vivo study.
Li X, Wang K, Ren Y, Zhang L, Tang XJ, Zhang HM, Zhao CQ, Liu PJ, Zhang JM, He JJ
Cell death & disease 2014 Jul 31;5(7):e1356
Cell death & disease 2014 Jul 31;5(7):e1356
PPE38 of Mycobacterium marinum triggers the cross-talk of multiple pathways involved in the host response, as revealed by subcellular quantitative proteomics.
Wang H, Dong D, Tang S, Chen X, Gao Q
Journal of proteome research 2013 May 3;12(5):2055-66
Journal of proteome research 2013 May 3;12(5):2055-66
Checkpoint kinase 1 (Chk1)-short is a splice variant and endogenous inhibitor of Chk1 that regulates cell cycle and DNA damage checkpoints.
Pabla N, Bhatt K, Dong Z
Proceedings of the National Academy of Sciences of the United States of America 2012 Jan 3;109(1):197-202
Proceedings of the National Academy of Sciences of the United States of America 2012 Jan 3;109(1):197-202
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Supportive validation
- Submitted by
- Proteintech Group (provider)
- Main image
- Experimental details
- mouse thymus tissue were subjected to SDS PAGE followed by western blot with 10362-1-AP(CHK1 antibody) at dilution of 1:1000
- Sample type
- tissue
Supportive validation
- Submitted by
- Proteintech Group (provider)
- Main image
- Experimental details
- The Chk1 antibody from Proteintech is a rabbit polyclonal antibody to a recombinant protein of human Chk1. This antibody recognizes human,mouse,rat antigen. The Chk1 antibody has been validated for the following applications: ELISA, WB, IF analysis.
