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Western blot
Immunoprecipitation
ImmunohistochemistryAntibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [2]
- Immunocytochemistry [3]
- Immunohistochemistry [2]
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- Product number
- LS-C287210 - Provider product page
- Provider
- LSBio
- Product name
- CHEK1 / CHK1 Antibody (aa250-300) LS-C287210
- Antibody type
- Polyclonal
- Description
- Affinity purified
- Reactivity
- Human
- Host
- Rabbit
- Isotype
- IgG
- Storage
- Store at 2-8°C for up to one year.
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Enhanced validation
- Submitted by
- LSBio (provider)
- Enhanced method
- Genetic validation
- Main image
- Experimental details
- Detection of Human Chk1 by Western Blot. Samples: Whole cell lysate (20 ug) from HeLa cells. Antibody: Affinity purified rabbit anti-Chk1 antibody used at the indicated concentrations. Detection: Chemiluminescence with exposure times of 15 seconds and 1 hour.
- Submitted by
- LSBio (provider)
- Enhanced method
- Genetic validation
- Main image
- Experimental details
- Detection of human Chk1 by western blot. Samples: Whole cell lysate (50 µg) from HeLa, HEK293T, Jurkat, and mouse TMCK cells. Antibodies: Affinity purified rabbit anti-Chk1 antibody used for WB at 0.4 µg/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.
Enhanced validation
- Submitted by
- LSBio (provider)
- Enhanced method
- Genetic validation
- Main image
- Experimental details
- Detection of Human Chk1 by Immunocytochemistry. Samples: NBF-fixed HeLa cells treated with neocarzinostatin. Antibody: Affinity purified rabbit anti-Chk1 used at a dilution of 1:1000 (1 ug/ml). Detection: Anti-rabbit IgG-FITC conjugated used at a dilution of 1:100.
- Submitted by
- LSBio (provider)
- Main image
- Experimental details
- Detection of Human Chk1 by Immunocytochemistry. Samples: NBF-fixed HeLa cells treated with neocarzinostatin. Antibody: Affinity purified rabbit anti-Chk1 used at a dilution of 1:1000 (1 ug/ml). Detection: Anti-rabbit IgG-FITC conjugated used at a dilution of 1:100.
- Submitted by
- LSBio (provider)
- Main image
- Experimental details
- Detection of Human Chk1 by Immunocytochemistry. Samples: NBF-fixed HeLa cells treated with neocarzinostatin. Antibody: Affinity purified rabbit anti-Chk1 used at a dilution of 1:1000 (1 ug/ml). Detection: Anti-rabbit IgG-FITC conjugated used at a dilution of 1:100.
Supportive validation
- Submitted by
- LSBio (provider)
- Enhanced method
- Genetic validation
- Main image
- Experimental details
- Detection of Human Chk1 by Immunohistochemistry. Sample: FFPE section of human testicular seminoma. Antibody: Affinity purified rabbit anti-Chk1 used at a dilution of 1:1000 (1 ug/ml).
- Submitted by
- LSBio (provider)
- Enhanced method
- Genetic validation
- Main image
- Experimental details
- Detection of human CHK1 by immunohistochemistry. Sample: FFPE section of human breast carcinoma. Antibody: Affinity purified rabbit anti-CHK1 used at a dilution of 1:1,000 (1µg/ml). Detection: DAB
