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Western blotAntibody data
- Antibody Data
- Antigen structure
- References [2]
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- Validations
- Western blot [2]
- Immunocytochemistry [1]
- Immunohistochemistry [1]
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- Product number
- GTX100070 - Provider product page
- Provider
- GeneTex
- Proper citation
- GeneTex Cat#GTX100070, RRID:AB_1080957
- Product name
- Chk1 antibody [C1C2-6], Internal
- Antibody type
- Polyclonal
- Reactivity
- Human
- Host
- Rabbit
Submitted references p22phox confers resistance to cisplatin, by blocking its entry into the nucleus.
A novel synthetic protoapigenone analogue, WYC02-9, induces DNA damage and apoptosis in DU145 prostate cancer cells through generation of reactive oxygen species.
Hung CC, Chien CY, Chiang WF, Lin CS, Hour TC, Chen HR, Wang LF, Ko JY, Chang CH, Chen JY
Oncotarget 2015 Feb 28;6(6):4110-25
Oncotarget 2015 Feb 28;6(6):4110-25
A novel synthetic protoapigenone analogue, WYC02-9, induces DNA damage and apoptosis in DU145 prostate cancer cells through generation of reactive oxygen species.
Chen HM, Chang FR, Hsieh YC, Cheng YJ, Hsieh KC, Tsai LM, Lin AS, Wu YC, Yuan SS
Free radical biology & medicine 2011 May 1;50(9):1151-62
Free radical biology & medicine 2011 May 1;50(9):1151-62
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Supportive validation
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- GeneTex (provider)
- Main image
- Experimental details
- Sample A: 20 ug of 293T nuclear extract 7.5% SDS PAGE GTX100070 diluted at 1:1000
- Validation comment
- WB
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- Chk1 antibody [C1C2-6], Internal detects Chk1 protein by western blot analysis.A. 30 £gg 293T whole cell extract B. 30 £gg whole cell extract of human Chk1-transfected 293T cells10 % SDS-PAGEChk1 antibody [C1C2-6], Internal (GTX100070) dilution: 1:5000
Supportive validation
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- CHK1 antibody [C1C2-6], Internal detects Chk1 protein at nucleus by immunofluorescent analysis.Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min.Green: Chk1 protein stained by CHK1 antibody [C1C2-6], Internal (GTX100070) diluted at 1:500.Red: alpha Tubulin, a cytoskeleton marker, stained by alpha Tubulin antibody [GT114] (GTX628802) diluted at 1:1000.Blue: Hoechst 33342 staining.
Supportive validation
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- GeneTex (provider)
- Main image
- Experimental details
- Immunohistochemical analysis of paraffin-embedded SCM-1 xenograft, using Chk1(GTX100070) antibody at 1:100 dilution.
