25887-1-AP

antibody from Proteintech Group

Targeting: CHEK1 CHK1

Provider product page for 25887-1-AP

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Immunocytochemistry

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Antibody data

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Antigen structure
References [58]
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Product number: 25887-1-AP - Provider product page
Provider: Proteintech Group
Product name: Chk1 antibody
Antibody type: Polyclonal
Description: Chk1 antibody (Cat. #25887-1-AP) is a rabbit polyclonal antibody that shows reactivity with human and has been validated for the following applications: FC, IF, IHC, IP, WB, ELISA.
Reactivity: Human
Host: Rabbit
Conjugate: Unconjugated
Isotype: IgG
Vial size: 20ul, 150ul

CHEK1 protein structure - 25887-1-AP shown in red.

Submitted references Overexpression of CDT1 inhibits cell cycle progression at S phase by interacting with the mini-chromosome maintenance complex and causes DNA damage.

Tsuyama T, Takayama N, Tanaka R, Arai Y, Yamaguchi Y, Nawata Y, Azuma Y, Tada S
FEBS open bio 2026 Feb;16(2):352-364

ATR inhibition potentiates the antitumor efficacy of HER3-DXd in HER3-positive/HR-positive breast cancer by increasing DNA damage.

Xie X, Lee J, Gi YJ, Poullikkas T, Fuson JA, Fan PD, Fukui JA, Tripathy D, Ueno NT
British journal of cancer 2026 Apr 15;

Cyclin Y interacts with Chk1 to activate RRM2/STAT3 signaling and promotes radioresistance in non-small cell lung cancer.

Liu Z, Xue H, Wang Z, Zhao Y, Xu S, Dong X
International journal of biological sciences 2025;21(5):1999-2011

Predicting breast cancer prognosis based on a novel pathomics model through CHEK1 expression analysis using machine learning algorithms.

Chen C, Gao D, Yue H, Wang H, Qu R, Hu X, Luo L
PloS one 2025;20(5):e0321717

YX-112, a novel celastrol-derived PROTAC, inhibits the development of triple-negative breast cancer by targeting the degradation of multiple proteins.

Gu Y, Yang M, Wang W, Li L, Ma Y, Liu W, Zhao Q
Frontiers in pharmacology 2025;16:1571135

CHK1-S, a splicing variant of CHK1, suppresses chronic myeloid leukemia.

Lei H, Xu H, Wang Y, Wu Y
Leukemia research 2025 Sep;156:107922

Cholangiocytes' primary cilia regulate DNA damage response and repair.

Peixoto E, Pant K, Richard S, Popoca JP, Abrahante JE, Czaja W, Gradilone SA
American journal of physiology. Gastrointestinal and liver physiology 2025 Sep 1;329(3):G469-G483

RSL3 promotes PARP1 apoptotic functions by distinct mechanisms during ferroptosis.

Chen D, Xie F, Mo Y, Qin D, Zheng B, Chen L
Cellular & molecular biology letters 2025 Oct 2;30(1):109

Combined CHK1 and PD-L1 blockade as a novel therapeutic strategy against stemness and immunosuppression in ovarian cancer.

Chen M, Huang L, Zhu M, Cai J, Ying F, Liu L, Li W, Sun S, Wen Y
Cancer immunology, immunotherapy : CII 2025 Nov 6;74(12):365

Icaritin Represses Autophagy to Promote Colorectal Cancer Cell Apoptosis and Sensitized Low-Temperature Photothermal Therapy via Targeting HSP90-TXNDC9 Interactions.

He D, Chen S, Wang X, Wen X, Gong C, Liu L, He G
Advanced science (Weinheim, Baden-Wurttemberg, Germany) 2025 May;12(20):e2412953

Tailoring an intravenously injectable oncolytic virus for augmenting radiotherapy.

Xu C, Chen L, Liu G, Xu J, Lv W, Gao X, Xu P, Tang M, Wang Y, Zhao X, Nie G, Cheng K, Liu F
Cell reports. Medicine 2025 May 20;6(5):102078

RBM17 Promotes the Chemoresistance of Oral Squamous Cancer Cells Through Checkpoint Kinase 1.

Nakahara M, Arai R, Tokuoka I, Fukumura K, Mayeda A, Yashiro M, Nakahara H
International journal of molecular sciences 2025 Mar 28;26(7)

Prexasertib exerts a synergistic effect on the antitumor activity of Lenvatinib through ALOX15-mediated ferroptosis in hepatocellular carcinoma.

Zhang S, Guan M, Ren T, Li N, Ding Q, Sun D, Zhu H
International immunopharmacology 2025 Mar 26;150:114278

Effect of PRKD3 on cell cycle in gastric cancer progression and downstream regulatory networks.

Wang S, Xie B, Deng H, Ma X, Tang B, Ma L, Zhu J, Li J, Li L
Medical oncology (Northwood, London, England) 2025 Mar 25;42(5):135

ZNF451 collaborates with RNF8 to regulate RNF168 localization and amplify ubiquitination signaling to promote DNA damage repair and regulate radiosensitivity.

Xu F, Xia Q, Chen B, Wang R, Zhang J, Zhao X, Zhang Z, Yao Z, Zhang J, Zhou S, Li X, Chen B, Xu A, Wu L, Zhao G
Cell death and differentiation 2025 Jul;32(7):1303-1316

Cholangiocytes' Primary Cilia Regulate DNA Damage Response and Repair.

Peixoto E, Pant K, Richard S, Abrahante JE, Czaja W, Gradilone SA
bioRxiv : the preprint server for biology 2025 Feb 16;

Abrogation of aberrant glycolytic interactions eliminates senescent cells and alleviates aging-related dysfunctions.

Mikawa T, Kameda M, Ikari S, Shibata E, Liu S, Miyagawa S, Ono K, Ito T, Yoshizawa A, Sugimoto M, Shibuya S, Shimizu T, Almunia J, Ogiso N, Revêchon G, Palazzo A, Bernard D, Kanda H, Soga T, Takubo K, Morioka S, Sasaki J, Sasaki T, Itamoto A, Fujii T, Seno H, Inagaki N, Kondoh H
Signal transduction and targeted therapy 2025 Dec 15;10(1):402

CHK1 inhibition increases the therapeutic response to radiotherapy via antitumor immunity in ARID1A-deficient colorectal cancer.

Chen JY, Ke TW, Chiang SF, Hong WZ, Chang HY, Liang JA, Tsai YY, Huang CH, Chen WT, Chao KSC, Huang KC
Cell death & disease 2025 Aug 1;16(1):584

EGFR tyrosine kinase inhibitor ZZC4 overcomes acquired resistance to gefitinib.

Attiogbe MKI, Huang TT, Zhao HY, Wang HY, Cao L, Yan PP, Zhang SQ, Cao YX
Toxicology and applied pharmacology 2025 Apr;497:117280

Structurally diverse tetrahydroxanthone analogues from Paraconiothyrium sp. AC31 with pyroptosis induction through targeted inhibition of PARP1 in hepatocellular carcinoma cells.

Zhang MK, Hu QQ, He LM, Li M, Chen WC, Zhu KK, Yuan RY, Wu XY, Gao P, Zeng XB, Cai YS
Bioorganic chemistry 2025 Apr;157:108310

Kaempferol Induces DNA Damage in Colorectal Cancer Cells by Regulating the MiR-195/miR-497-PFKFB4-Mediated Nonoxidative Pentose Phosphate Pathway.

Wu H, Du J, Hu P, Wang P, Wang Z, Ma J, Li Z
Journal of agricultural and food chemistry 2025 Apr 9;73(14):8312-8322

HIF-1α-HPRT1 axis promotes tumorigenesis and gefitinib resistance by enhancing purine metabolism in EGFR-mutant lung adenocarcinoma.

Geng P, Ye F, Dou P, Hu C, He J, Zhao J, Li Q, Bao M, Li X, Liu X, Xu G
Journal of experimental & clinical cancer research : CR 2024 Sep 30;43(1):269

MRE11A: a novel negative regulator of human DNA mismatch repair.

Du D, Yang Y, Zhang Y, Wang G, Chen L, Guan X, Rasmussen LJ, Liu D
Cellular & molecular biology letters 2024 Mar 14;29(1):37

Amentoflavone reverses epithelial-mesenchymal transition in hepatocellular carcinoma cells by targeting p53 signalling pathway axis.

Jian HY, Zhang JT, Liu Z, Zhang Z, Zeng PH
Journal of cellular and molecular medicine 2024 Jun;28(11):e18442

CDK5RAP3 is a novel super-enhancer-driven gene activated by master TFs and regulates ER-Phagy in neuroblastoma.

Zhuo R, Zhang Z, Chen Y, Li G, Du S, Guo X, Yang R, Tao Y, Li X, Fang F, Xie Y, Wu D, Yang Y, Yang C, Yin H, Qian G, Wang H, Yu J, Jia S, Zhu F, Feng C, Wang J, Xu Y, Li Z, Shi L, Wang X, Pan J, Wang J
Cancer letters 2024 Jun 1;591:216882

Alphaherpesvirus manipulates retinoic acid metabolism for optimal replication.

Ming S, Zhang S, Xing J, Yang G, Zeng L, Wang J, Chu B
iScience 2024 Jul 19;27(7):110144

OTUB1 Targets CHK1 for Deubiquitination and Stabilization to Facilitate Lung Cancer Progression and Radioresistance.

Wang J, Liu Y, Wu D, Tian C, Gao J, Yang Q, Hong X, Gu F, Zhang K, Hu Y, Xu S, Liu L, Zeng Y
International journal of radiation oncology, biology, physics 2024 Jul 15;119(4):1222-1233

m(3)C32 tRNA modification controls serine codon-biased mRNA translation, cell cycle, and DNA-damage response.

Cui J, Sendinc E, Liu Q, Kim S, Fang JY, Gregory RI
Nature communications 2024 Jul 10;15(1):5775

Coptisine-mediated downregulation of E2F7 induces G2/M phase arrest in hepatocellular carcinoma cells through inhibition of E2F4/NFYA/NFYB transcription factors.

Wang H, Ma Z, Xu M, Xiong M, Chen X, Zhou Y, Tang W, Li X, Chen W, Ma H, Ye X
Chemico-biological interactions 2024 Jul 1;397:111063

Inactivation of the TGF-β1/ALK5 axis enhances club cell function and alleviates lung tissue damage to ameliorate COPD progression through the MEK/ERK signaling pathway.

Tian J, Ouyang H, Wu J, Wen L, Li X, Yang F, Yuan H
General physiology and biophysics 2024 Jan;43(1):37-48

AZD7762 induces CRBN dependent BAG3 degradation through ubiquitin-proteasome pathway.

Liao Y, Yuan C, Huang M, Si W, Li D, Wu W, Zhang S, Wu R, Quan Y, Yu X, Liao S
Anti-cancer drugs 2024 Jan 1;35(1):46-54

Synergistic effect of adavosertib and fimepinostat on acute myeloid leukemia cells by enhancing the induction of DNA damage.

Wang Y, Lin X, Wang Y, Wang G
Investigational new drugs 2024 Feb;42(1):70-79

Isovalerylspiramycin I suppresses small cell lung cancer proliferation via ATR/CHK1 mediated DNA damage response and PERK/eIF2α/ATF4/CHOP mediated ER stress.

Xu Y, Gu X, Shan S, Liu Z, Wang S, Zhang J, Lei Y, Zhong C, Zheng Q, Ren T, Li Z
Biochemical pharmacology 2024 Dec;230(Pt 1):116557

SARS-CoV-2 N protein-induced Dicer, XPO5, SRSF3, and hnRNPA3 downregulation causes pneumonia.

Luo YW, Zhou JP, Ji H, Xu D, Zheng A, Wang X, Dai Z, Luo Z, Cao F, Wang XY, Bai Y, Chen D, Chen Y, Wang Q, Yang Y, Zhang X, Chiu S, Peng X, Huang AL, Tang KF
Nature communications 2024 Aug 13;15(1):6964

CDC20 Holds Novel Regulation Mechanism in RPA1 during Different Stages of DNA Damage to Induce Radio-Chemoresistance.

Gao Y, Wen P, Shao C, Ye C, Chen Y, You J, Su Z
International journal of molecular sciences 2024 Aug 1;25(15)

The SPATA5-SPATA5L1 ATPase complex directs replisome proteostasis to ensure genome integrity.

Krishnamoorthy V, Foglizzo M, Dilley RL, Wu A, Datta A, Dutta P, Campbell LJ, Degtjarik O, Musgrove LJ, Calabrese AN, Zeqiraj E, Greenberg RA
Cell 2024 Apr 25;187(9):2250-2268.e31

Nanoengineered Gallium Ion Incorporated Formulation for Safe and Efficient Reversal of PARP Inhibition and Platinum Resistance in Ovarian Cancer.

Li Y, Cen Y, Tu M, Xiang Z, Tang S, Lu W, Zhang H, Xu J
Research (Washington, D.C.) 2023;6:0070

Immortalization-upregulated protein promotes pancreatic cancer progression by regulating NPM1/FHL1-mediated cell-cycle-checkpoint protein activity.

Luo Q, Pan Y, Fu Q, Zhang X, Zhou S, Yu P, Tian H, Liu P, Chen S, Zhang H, Qin T
Cell biology and toxicology 2023 Oct;39(5):2069-2087

The ATR inhibitor VE-821 increases the sensitivity of gastric cancer cells to cisplatin.

Su H, Yuan Y, Tang J, Zhang Y, Wu H, Zhang Y, Liang J, Wang L, Zou X, Huang S, Zhang S, Lv Y
Translational oncology 2023 Oct;36:101743

ADAR1 links R-loop homeostasis to ATR activation in replication stress response.

Zhang B, Li Y, Zhang J, Wang Y, Liang C, Lu T, Zhang C, Liu L, Qin Y, He J, Zhao X, Yu J, Hao J, Yang J, Li MJ, Yao Z, Ma S, Cheng H, Cheng T, Shi L
Nucleic acids research 2023 Nov 27;51(21):11668-11687

C11orf54 promotes DNA repair via blocking CMA-mediated degradation of HIF1A.

Tan J, Wang W, Liu X, Xu J, Che Y, Liu Y, Hu J, Hu L, Li J, Zhou Q
Communications biology 2023 Jun 5;6(1):606

Inhibition of Checkpoint Kinase 1 (CHK1) Upregulates Interferon Regulatory Factor 1 (IRF1) to Promote Apoptosis and Activate Anti-Tumor Immunity via MICA in Hepatocellular Carcinoma (HCC).

Li X, Huang J, Wu Q, Du Q, Wang Y, Huang Y, Cai X, Geller DA, Yan Y
Cancers 2023 Jan 30;15(3)

Loss of phosphatase CTDNEP1 potentiates aggressive medulloblastoma by triggering MYC amplification and genomic instability.

Luo Z, Xin D, Liao Y, Berry K, Ogurek S, Zhang F, Zhang L, Zhao C, Rao R, Dong X, Li H, Yu J, Lin Y, Huang G, Xu L, Xin M, Nishinakamura R, Yu J, Kool M, Pfister SM, Roussel MF, Zhou W, Weiss WA, Andreassen P, Lu QR
Nature communications 2023 Feb 10;14(1):762

SIRT3-dependent mitochondrial redox homeostasis mitigates CHK1 inhibition combined with gemcitabine treatment induced cardiotoxicity in hiPSC-CMs and mice.

Chen JW, Shan TK, Wei TW, Jiang QQ, Du C, Gu LF, Yang TT, Zhou LH, Wang SB, Bao YL, Wang H, Ji Y, Xie LP, Gu AH, Sun CQ, Wang QM, Wang LS
Archives of toxicology 2023 Dec;97(12):3209-3226

Combined Inactivation of CTPS1 and ATR Is Synthetically Lethal to MYC-Overexpressing Cancer Cells.

Sun Z, Zhang Z, Wang QQ, Liu JL
Cancer research 2022 Mar 15;82(6):1013-1024

Inhibition of STAT3(Y705) phosphorylation by Stattic suppresses proliferation and induces mitochondrial-dependent apoptosis in pancreatic cancer cells.

Guo H, Xiao Y, Yuan Z, Yang X, Chen J, Chen C, Wang M, Xie L, Chen Q, Tong Y, Zhang Q, Bai Y
Cell death discovery 2022 Mar 14;8(1):116

G-quadruplex DNA structures in human stem cells and differentiation.

Zyner KG, Simeone A, Flynn SM, Doyle C, Marsico G, Adhikari S, Portella G, Tannahill D, Balasubramanian S
Nature communications 2022 Jan 10;13(1):142

Deubiquitinase OTUD6A promotes breast cancer progression by increasing TopBP1 stability and rendering tumor cells resistant to DNA-damaging therapy.

Zhao Y, Huang X, Zhu D, Wei M, Luo J, Yu S, Tian Y, Zheng X
Cell death and differentiation 2022 Dec;29(12):2531-2544

Corynoxine suppresses pancreatic cancer growth primarily via ROS-p38 mediated cytostatic effects.

Wen C, Ruan Q, Li Z, Zhou X, Yang X, Xu P, Akuetteh PDP, Xu Z, Deng J
British journal of cancer 2022 Dec;127(12):2108-2117

ACTL6A deficiency induces apoptosis through impairing DNA replication and inhibiting the ATR-Chk1 signaling in glioblastoma cells.

Hu X, Pei D, Ci M, Zhang G, Li B, Wang J, Shen Y, Zhai X, Liang P, Cui H
Biochemical and biophysical research communications 2022 Apr 9;599:148-155

Induction of Mitosis Delay and Apoptosis by CDDO-TFEA in Glioblastoma Multiforme.

Tsai TH, Lieu AS, Huang TY, Kwan AL, Lin CL, Hsu YC
Frontiers in pharmacology 2021;12:756228

[Development and functional validation of a nano-delivery system of miR-16/polypeptide targeting ovarian cancer cells].

Li Q, Cen B, Huang W, Chen J, Chen Z, Pang J, Fu W, He S, Ji A
Nan fang yi ke da xue xue bao = Journal of Southern Medical University 2021 May 20;41(5):736-746

The HDAC and PI3K dual inhibitor CUDC-907 synergistically enhances the antileukemic activity of venetoclax in preclinical models of acute myeloid leukemia.

Li X, Su Y, Hege K, Madlambayan G, Edwards H, Knight T, Polin L, Kushner J, Dzinic SH, White K, Yang J, Miller R, Wang G, Zhao L, Wang Y, Lin H, Taub JW, Ge Y
Haematologica 2021 May 1;106(5):1262-1277

MAP4K1 functions as a tumor promotor and drug mediator for AML via modulation of DNA damage/repair system and MAPK pathway.

Ling Q, Li F, Zhang X, Mao S, Lin X, Pan J, Ye W, Wei W, Qian Y, Hu C, Huang X, Wang J, Wang H, Huang J, Wang Y, Jin J
EBioMedicine 2021 Jul;69:103441

Azidothymidine (AZT) Inhibits Proliferation of Human Ovarian Cancer Cells by Regulating Cell Cycle Progression.

Hsieh Y, Tseng JJ
Anticancer research 2020 Oct;40(10):5517-5527

UBE2T-regulated H2AX monoubiquitination induces hepatocellular carcinoma radioresistance by facilitating CHK1 activation.

Sun J, Zhu Z, Li W, Shen M, Cao C, Sun Q, Guo Z, Liu L, Wu D
Journal of experimental & clinical cancer research : CR 2020 Oct 21;39(1):222

A novel alkylating deacetylase inhibitor molecule EDO-S101 in combination with cytarabine synergistically enhances apoptosis of acute myeloid leukemia cells.

Jin J, Mao S, Li F, Li X, Huang X, Yu M, Guo W, Jin J
Medical oncology (Northwood, London, England) 2019 Aug 1;36(9):77

RNF126 as a Biomarker of a Poor Prognosis in Invasive Breast Cancer and CHEK1 Inhibitor Efficacy in Breast Cancer Cells.

Yang X, Pan Y, Qiu Z, Du Z, Zhang Y, Fa P, Gorityala S, Ma S, Li S, Chen C, Wang H, Xu Y, Yan C, Ruth K, Ma Z, Zhang J
Clinical cancer research : an official journal of the American Association for Cancer Research 2018 Apr 1;24(7):1629-1643

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