NB100-464

antibody from Novus Biologicals

Targeting: CHEK1 CHK1

Western blot (Supportive data in Antibodypedia)

Immunoprecipitation (Supportive data in Antibodypedia)

Immunohistochemistry (Supportive data in Antibodypedia)

Antibody Data

Product number

NB100-464

Provider

Novus Biologicals

Proper citation

Novus Cat#NB100-464, RRID:AB_10002158

Product name

Rabbit Polyclonal Chk1 Antibody

Antibody type

Polyclonal

Description

Immunogen affinity purified.

Reactivity

Human, Mouse

Host

Rabbit

Isotype

IgG

Vial size

100 ul

Concentration

1.0 mg/ml

Storage

Store at 4C. Do not freeze.

References

Submitted references

Serine/Threonine Kinase CHEK1-Dependent Transcriptional Regulation of RAD54L Promotes Proliferation and Radio Resistance in Glioblastoma.

Bai X, Wang J, Huo L, Xie Y, Xie W, Xu G, Wang M
Translational oncology 2018 Feb;11(1):140-146

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Two subunits of human ORC are dispensable for DNA replication and proliferation.

Shibata E, Kiran M, Shibata Y, Singh S, Kiran S, Dutta A
eLife 2016 Dec 1;5

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Over expression of hRad9 protein correlates with reduced chemosensitivity in breast cancer with administration of neoadjuvant chemotherapy.

Yun H, Shi R, Yang Q, Zhang X, Wang Y, Zhou X, Mu K
Scientific reports 2014 Dec 18;4:7548

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Overcoming platinum resistance in preclinical models of ovarian cancer using the neddylation inhibitor MLN4924.

Jazaeri AA, Shibata E, Park J, Bryant JL, Conaway MR, Modesitt SC, Smith PG, Milhollen MA, Berger AJ, Dutta A
Molecular cancer therapeutics 2013 Oct;12(10):1958-67

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Degradation of p12 subunit by CRL4Cdt2 E3 ligase inhibits fork progression after DNA damage.

Terai K, Shibata E, Abbas T, Dutta A
The Journal of biological chemistry 2013 Oct 18;288(42):30509-14

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CHK1 targets spleen tyrosine kinase (L) for proteolysis in hepatocellular carcinoma.

Hong J, Hu K, Yuan Y, Sang Y, Bu Q, Chen G, Yang L, Li B, Huang P, Chen D, Liang Y, Zhang R, Pan J, Zeng YX, Kang T
The Journal of clinical investigation 2012 Jun;122(6):2165-75

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The effect of the intra-S-phase checkpoint on origins of replication in human cells.

Karnani N, Dutta A
Genes & development 2011 Mar 15;25(6):621-33

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Involvement of the ATR- and ATM-dependent checkpoint responses in cell cycle arrest evoked by pierisin-1.

Shiotani B, Kobayashi M, Watanabe M, Yamamoto K, Sugimura T, Wakabayashi K
Molecular cancer research : MCR 2006 Feb;4(2):125-33

Western blot

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Western Blot: Chk1 Antibody [NB100-464] - Detection of Human Chk1 by Western Blot. Samples: Whole cell lysate (50 ug) from HeLa, 293T, Jurkat, and mouse TMCK cells. Antibodies: Affinity purified rabbit anti-Chk1 antibody NB100-464 used for WB at 0.4 ug/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.

Immunoprecipitation

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Immunoprecipitation: Chk1 Antibody [NB100-464] - Detection of human Chk1 by western blot of immunoprecipitates. Samples: Whole cell lysate (1 mg for IP; 20% of IP loaded) from HeLa cells. Antibodies: Affinity purified rabbit anti-Chk1 antibody NB100-464 (lot 2) used for IP at 6 ug/mg lysate. Chk1 was also immunoprecipitated by a previous lot (lot 1) of this antibody. For blotting immunoprecipitated Chk1, NB100-464 (lot 2) was used at 1 ug/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.

Immunohistochemistry

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Immunohistochemistry-Paraffin: Chk1 Antibody [NB100-464] - Section of human breast carcinoma. Antibody: Affinity purified rabbit anti-CHK1 used at a dilution of 1:1,000 (1ug/ml). Detection: DAB