16612-1-AP

antibody from Proteintech Group

Targeting: BCL2L13 BCL-RAMBO, MIL1

Provider product page for 16612-1-AP

Western blot

ELISA

Immunocytochemistry

Immunoprecipitation

Immunohistochemistry

Antibody data

Antibody Data
Antigen structure
References [31]
Comments [0]
Validations
Western blot [1]
Immunocytochemistry [1]
Immunohistochemistry [2]

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Product number: 16612-1-AP - Provider product page
Provider: Proteintech Group
Proper citation: Proteintech Cat#16612-1-AP, RRID:AB_1850928
Product name: BCL2L13 antibody
Antibody type: Polyclonal
Description: KD/KO validated BCL2L13 antibody (Cat. #16612-1-AP) is a rabbit polyclonal antibody that shows reactivity with human, mouse, rat and has been validated for the following applications: IF, IHC, IP, WB,ELISA.
Reactivity: Human, Mouse, Rat
Host: Rabbit
Conjugate: Unconjugated
Isotype: IgG
Vial size: 20ul, 150ul

BCL2L13 protein structure - 16612-1-AP shown in red.

Submitted references MAP2K6 directly phosphorylates BCL2L13 to mediate mitophagy for suppressing tumorigenicity.

Xing G, Huang Y, Liu X, Li L, Liu Z, Wu Y, Zhou Y, Ding Y, Chen B, Xue G, Li Z, Hao Z, Liu Y, Fan W, Ding H, Liang S, He J, Wang J, Zhang J, Qin D, Wang W, Lu G, Chan WY, Ran P, Ju H, Dong M, Liang W, Liu X, Chen K
Cell reports 2026 Mar 24;45(4):117177

Hypoxia-induced PRMT6 expression promotes temozolomide chemoresistance in glioblastoma via G3BP1.

Chen S, Yu P, Sun Y, Yan Y, Zhang B, Li G
Journal of translational medicine 2026 Jan 2;24(1):226

Enterovirus-induced cleavage of Mitofusin 2 generates mitophagosomes for enveloped virion release.

Jassey A, Paudel B, Wagner MA, Pollack N, Cheng IT, Godoy-Ruiz R, Weber DJ, Jackson WT
Science advances 2026 Apr 24;12(17):eaed6824

Protein phosphatase EYA1 regulates the dephosphorylation and turnover of BCL2L12 to promote glioma development.

Wei T, Lin R, Lu Y, Jin DY, Zhang J, Sham MH
International journal of biological sciences 2025;21(3):1081-1096

The mitophagy receptors BNIP3 and NIX mediate tight attachment and expansion of the isolation membrane to mitochondria.

Yamashita SI, Arai R, Hada H, Padman BS, Lazarou M, Chan DC, Kanki T, Waguri S
The Journal of cell biology 2025 Jul 7;224(7)

Multi-site phosphorylation of BCL2L13 in a TBK1- and AMPK-dependent manner reveals new modes of mitophagy regulation.

Ahmed H, Reynolds MB, Kasof G, Shadel GS, Shaw R
bioRxiv : the preprint server for biology 2025 Jul 11;

Sex and region-specific disruption of autophagy and mitophagy in Alzheimer's disease: linking cellular dysfunction to cognitive decline.

Adlimoghaddam A, Fayazbakhsh F, Mohammadi M, Babaei Z, Behrooz AB, Tabasi F, Guan T, Beheshti I, Aghaei M, Klionsky DJ, Albensi BC, Ghavami S
Cell death discovery 2025 Apr 26;11(1):204

Sex and Region-Specific Disruption of Autophagy and Mitophagy in Alzheimer's Disease: Linking Cellular Dysfunction to Cognitive Decline.

Adlimoghaddam A, Fayazbakhsh F, Mohammadi M, Babaei Z, Behrooz AB, Tabasi F, Guan T, Beheshti I, Aghaei M, Klionsky DJ, Albensi BC, Ghavami S
bioRxiv : the preprint server for biology 2024 Oct 31;

Mitochondrial tRNA(Glu) 14693A > G Mutation, an "Enhancer" to the Phenotypic Expression of Leber's Hereditary Optic Neuropathy.

Jin L, Gan D, He W, Wu N, Xiang S, Wei Y, Eriani G, Ji Y, Guan MX, Wang M
Advanced science (Weinheim, Baden-Wurttemberg, Germany) 2024 Nov;11(41):e2401856

Mitophagy mediated by BNIP3 and NIX protects against ferroptosis by downregulating mitochondrial reactive oxygen species.

Yamashita SI, Sugiura Y, Matsuoka Y, Maeda R, Inoue K, Furukawa K, Fukuda T, Chan DC, Kanki T
Cell death and differentiation 2024 May;31(5):651-661

Glucose-mediated mitochondrial reprogramming by cholesterol export at TM4SF5-enriched mitochondria-lysosome contact sites.

Kim JE, Park SY, Kwak C, Lee Y, Song DG, Jung JW, Lee H, Shin EA, Pinanga Y, Pyo KH, Lee EH, Kim W, Kim S, Jun CD, Yun J, Choi S, Rhee HW, Liu KH, Lee JW
Cancer communications (London, England) 2024 Jan;44(1):47-75

Mitophagosomes induced during EV-D68 infection promote viral nonlytic release.

Jassey A, Paudel B, Wagner MA, Pollack N, Cheng IT, Godoy-Ruiz R, Weber DJ, Jackson WT
bioRxiv : the preprint server for biology 2024 Dec 6;

AMPK regulates Bcl2-L-13-mediated mitophagy induction for cardioprotection.

Murakawa T, Ito J, Rusu MC, Taneike M, Omiya S, Moncayo-Arlandi J, Nakanishi C, Sugihara R, Nishida H, Mine K, Fleck R, Zhang M, Nishida K, Shah AM, Yamaguchi O, Sakata Y, Otsu K
Cell reports 2024 Dec 24;43(12):115001

BCL2L13 promotes mitophagy through DNM1L-mediated mitochondrial fission in glioblastoma.

Wang J, Chen A, Xue Z, Liu J, He Y, Liu G, Zhao Z, Li W, Zhang Q, Chen A, Wang J, Li X, Wang X, Huang B
Cell death & disease 2023 Sep 2;14(9):585

The mitophagy receptor BNIP3 is critical for the regulation of metabolic homeostasis and mitochondrial function in the nucleus pulposus cells of the intervertebral disc.

Madhu V, Hernandez-Meadows M, Boneski PK, Qiu Y, Guntur AR, Kurland IJ, Barve RA, Risbud MV
Autophagy 2023 Jun;19(6):1821-1843

A role for BCL2L13 and autophagy in germline purifying selection of mtDNA.

Kremer LS, Bozhilova LV, Rubalcava-Gracia D, Filograna R, Upadhyay M, Koolmeister C, Chinnery PF, Larsson NG
PLoS genetics 2023 Jan;19(1):e1010573

The protective effect of inhibiting mitochondrial fission on the juvenile rat brain following PTZ kindling through inhibiting the BCL2L13/LC3 mitophagy pathway.

Fang Q, Zheng S, Chen Q, Chen L, Yang Y, Wang Y, Zhang H, Chen J
Metabolic brain disease 2023 Feb;38(2):453-466

Profiling of purified autophagic vesicle degradome in the maturing and aging brain.

Kallergi E, Siva Sankar D, Matera A, Kolaxi A, Paolicelli RC, Dengjel J, Nikoletopoulou V
Neuron 2023 Aug 2;111(15):2329-2347.e7

Differential impact of doxorubicin dose on cell death and autophagy pathways during acute cardiotoxicity.

Kawalec P, Martens MD, Field JT, Mughal W, Caymo AM, Chapman D, Xiang B, Ghavami S, Dolinsky VW, Gordon JW
Toxicology and applied pharmacology 2022 Oct 15;453:116210

BNIP3 (BCL2 interacting protein 3) regulates pluripotency by modulating mitochondrial homeostasis via mitophagy.

Liu K, Zhao Q, Sun H, Liu L, Wang C, Li Z, Xu Y, Wang L, Zhang L, Zhang H, Chen Q, Zhao T
Cell death & disease 2022 Apr 11;13(4):334

BNIP3L/Nix-induced mitochondrial fission, mitophagy, and impaired myocyte glucose uptake are abrogated by PRKA/PKA phosphorylation.

da Silva Rosa SC, Martens MD, Field JT, Nguyen L, Kereliuk SM, Hai Y, Chapman D, Diehl-Jones W, Aliani M, West AR, Thliveris J, Ghavami S, Rampitsch C, Dolinsky VW, Gordon JW
Autophagy 2021 Sep;17(9):2257-2272

Down-regulation of BCL2L13 renders poor prognosis in clear cell and papillary renal cell carcinoma.

Meng F, Zhang L, Zhang M, Ye K, Guo W, Liu Y, Yang W, Zhai Z, Wang H, Xiao J, Dai H
Cancer cell international 2021 Jun 30;21(1):332

BNIP3L/NIX degradation leads to mitophagy deficiency in ischemic brains.

Wu X, Zheng Y, Liu M, Li Y, Ma S, Tang W, Yan W, Cao M, Zheng W, Jiang L, Wu J, Han F, Qin Z, Fang L, Hu W, Chen Z, Zhang X
Autophagy 2021 Aug;17(8):1934-1946

Neuroglial transmitophagy and Parkinson's disease.

Morales I, Sanchez A, Puertas-Avendaño R, Rodriguez-Sabate C, Perez-Barreto A, Rodriguez M
Glia 2020 Nov;68(11):2277-2299

Long non-coding RNA SNHG15 is a competing endogenous RNA of miR-141-3p that prevents osteoarthritis progression by upregulating BCL2L13 expression.

Zhang X, Huang CR, Pan S, Pang Y, Chen YS, Zha GC, Guo KJ, Zheng X
International immunopharmacology 2020 Jun;83:106425

The mitophagy receptor Bcl-2-like protein 13 stimulates adipogenesis by regulating mitochondrial oxidative phosphorylation and apoptosis in mice.

Fujiwara M, Tian L, Le PT, DeMambro VE, Becker KA, Rosen CJ, Guntur AR
The Journal of biological chemistry 2019 Aug 23;294(34):12683-12694

A novel role for Bcl2l13 in promoting beige adipocyte biogenesis.

Ju L, Chen S, Alimujiang M, Bai N, Yan H, Fang Q, Han J, Ma X, Yang Y, Jia W
Biochemical and biophysical research communications 2018 Nov 30;506(3):485-491

Degradation of altered mitochondria by autophagy is impaired in Lafora disease.

Lahuerta M, Aguado C, Sánchez-Martín P, Sanz P, Knecht E
The FEBS journal 2018 Jun;285(11):2071-2090

Low-dose rapamycin extends lifespan in a mouse model of mtDNA depletion syndrome.

Siegmund SE, Yang H, Sharma R, Javors M, Skinner O, Mootha V, Hirano M, Schon EA
Human molecular genetics 2017 Dec 1;26(23):4588-4605

Bcl-2-like protein 13 is a mammalian Atg32 homologue that mediates mitophagy and mitochondrial fragmentation.

Murakawa T, Yamaguchi O, Hashimoto A, Hikoso S, Takeda T, Oka T, Yasui H, Ueda H, Akazawa Y, Nakayama H, Taneike M, Misaka T, Omiya S, Shah AM, Yamamoto A, Nishida K, Ohsumi Y, Okamoto K, Sakata Y, Otsu K
Nature communications 2015 Jul 6;6:7527

Bcl2L13 is a ceramide synthase inhibitor in glioblastoma.

Jensen SA, Calvert AE, Volpert G, Kouri FM, Hurley LA, Luciano JP, Wu Y, Chalastanis A, Futerman AH, Stegh AH
Proceedings of the National Academy of Sciences of the United States of America 2014 Apr 15;111(15):5682-7

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Supportive validation

Submitted by: Proteintech Group (provider)
Main image
Experimental details: Jurkat cells were subjected to SDS PAGE followed by western blot with 16612-1-AP(BCL2L13 antibody) at dilution of 1:500
Sample type: cell line

Supportive validation

Submitted by: Proteintech Group (provider)
Main image
Experimental details: Immunofluorescent analysis of HepG2 cells, using BCL2L13 antibody 16612-1-AP at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red).
Sample type: cell line

Supportive validation

Submitted by: Proteintech Group (provider)
Main image
Experimental details: Immunohistochemical of paraffin-embedded human pancreas using 16612-1-AP(BCL2L13 antibody) at dilution of 1:100 (under 10x lens)
Sample type: tissue

Supportive validation

Submitted by: Proteintech Group (provider)
Main image
Experimental details: Immunohistochemical of paraffin-embedded human pancreas using 16612-1-AP(BCL2L13 antibody) at dilution of 1:100 (under 40x lens)
Sample type: tissue