A03496-1

antibody from Boster Biological Technology

Targeting: CRYAB CRYA2, HSPB5

Western blot (Data presented on provider website)

Immunocytochemistry (Data presented on provider website)

Immunohistochemistry (Supportive data in Antibodypedia)

Antibody Data

Product number

A03496-1

Provider

Boster Biological Technology

Product name

Anti-αB-Crystallin Antibody

Antibody type

Polyclonal

Description

Rabbit Polyclonal antibody for CRYAB detection. Tested positive for IF, IHC, WB in Human, Mouse, Rat

Reactivity

Human, Mouse, Rat

Host

Rabbit

Vial size

200µg

Concentration

0.5-1mg/ml, actual concentration vary by lot. Use suggested dilution ratio to decide dilution procedure.

Storage

-20°C

References

Submitted references

Independent pathways control muscle tissue size and sarcomere remodeling.

Brooks D, Bawa S, Bontrager A, Stetsiv M, Guo Y, Geisbrecht ER
Developmental biology 2022 Oct;490:1-12

Immunohistochemistry

Supportive validation

Submitted by

Boster Biological Technology (provider)

Main image

Experimental details

IHC analysis of CRYAB using anti-CRYAB antibody (A03496-1).CRYAB was detected in paraffin-embedded section. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1ug/ml rabbit anti-CRYAB Antibody (A03496-1) overnight at 4°C. Biotinylated goat anti Rabbit IgG antibody was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen.

Additional image