Explore
Validate
Learn
Western blotAntibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [2]
- Immunocytochemistry [2]
- Immunoprecipitation [1]
- Immunohistochemistry [2]
- Flow cytometry [1]
Submit
Validation data
Reference
Comment
Report error
- Product number
- TA500581 - Provider product page
- Provider
- OriGene
- Proper citation
- OriGene Cat#TA500581, RRID:AB_10585876
- Product name
- CRYAB (Alpha B Crystallin) mouse monoclonal antibody, clone OTI6A9 (formerly 6A9)
- Antibody type
- Monoclonal
- Description
- CRYAB (Alpha B Crystallin) mouse monoclonal antibody, clone OTI6A9 (formerly 6A9)
- Host
- Mouse
- Conjugate
- Unconjugated
- Epitope
- CRYAB
- Isotype
- IgG
- Antibody clone number
- OTI6A9
- Vial size
- 100 µl
- Concentration
- 1 mg/ml
No comments: Submit comment
Supportive validation
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- Western blot analysis of extracts (35ug) from 9 different cell lines by usin g anti-CRYAB monoclonal antibody (HepG2: human; HeLa: human; SVT2: mouse; A549: human; COS7: monkey; Jurkat: human; MDCK: canine; PC12: rat; MCF7: human).
- Validation comment
- WB
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY CRYAB (RC202718, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-CRYAB.
- Validation comment
- WB
Supportive validation
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- Immunofluorescent staining of HT29 cells using anti-CRYAB mouse monoclonal antibody (TA500581).
- Validation comment
- IF
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- Anti-CRYAB mouse monoclonal antibody (TA500581) immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY CRYAB(RC202718).
- Validation comment
- IF
Supportive validation
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- Immunoprecipitation(IP) of CRYAB by using TrueMab monoclonal anti-CRYAB antibodies (Negative control: IP without adding anti-CRYAB antibody.). For each experiment, 500ul of DDK tagged CRYAB overexpression lysates (at 1:5 dilution with HEK293T lysate), 2ug of anti-CRYAB antibody and 20ul (0.1mg) of goat anti-mouse conjugated magnetic beads were mixed and incubated overnight. After extensive wash to remove any non-specific binding, the immuno-precipitated products were analyzed with rabbit anti-DDK polyclonal antibody.
- Validation comment
- IP
Supportive validation
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- Immunohistochemical staining of paraffin-embedded Human endometrium tissue within the normal limits using anti-CRYAB mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA500581)
- Validation comment
- IHC
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- Immunohistochemical staining of paraffin-embedded Human pancreas tissue within the normal limits using anti-CRYAB mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA500581)
- Validation comment
- IHC
Supportive validation
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- HEK293T cells transfected with either RC202718 overexpress plasmid(Red) or empty vector control plasmid(Blue) were immunostained by anti-CRYAB antibody(TA500581), and then analyzed by flow cytometry.
- Validation comment
- FC
