Antibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [1]
- Immunocytochemistry [1]
- Immunohistochemistry [1]
- Flow cytometry [1]
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Validation data
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- Product number
- AP2872b - Provider product page
- Provider
- Abgent
- Proper citation
- Abgent Cat#AP2872b, RRID:AB_2120148
- Product name
- HSPA8 Antibody (C-term)
- Antibody type
- Polyclonal
- Antigen
- Synthetic peptide
- Description
- Purified Rabbit Polyclonal Antibody (Pab)
- Reactivity
- Human
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 400 µl
- Concentration
- 2 mg/ml
- Storage
- Maintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
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Supportive validation
- Submitted by
- Abgent (provider)
- Main image
- Experimental details
- "Western blot analysis of lysates from H-4-II-E, mouse NIH/3T3 cell line (from left to right), using HSPA8 Antibody (C-term) (Cat. # AP2872b). AP2872b was diluted at 1:1000 at each lane. A goat anti-rabbit IgG H&L(HRP) at 1:5000 dilution was used as the secondary antibody. Lysates at 35ug per lane."
- Primary Ab dilution
- 1:1000
Supportive validation
- Submitted by
- Abgent (provider)
- Main image
- Experimental details
- Confocal immunofluorescent analysis of HSPA8 Antibody (C-term)(Cat#AP2872b) with Hela cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green). Actin filaments have been labeled with Alexa Fluor 555 phalloidin (red).
- Primary Ab dilution
- 1:10~50
Supportive validation
- Submitted by
- Abgent (provider)
- Main image
- Experimental details
- HSPA8 Antibody (C-term) (Cat. #AP2872b)immunohistochemistry analysis in formalin fixed and paraffin embedded human stomach tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.This data demonstrates the use of HSPA8 Antibody (C-term) for immunohistochemistry. Clinical relevance has not been evaluated.
- Primary Ab dilution
- 1:10~50
Supportive validation
- Submitted by
- Abgent (provider)
- Main image
- Experimental details
- HSPA8 Antibody (C-term) (Cat. #AP2872b) flow cytometric analysis of Hela cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
- Primary Ab dilution
- 1:10~50