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Western blotAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [4]
- Immunocytochemistry [1]
- Immunohistochemistry [3]
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- Product number
- GTX101144 - Provider product page
- Provider
- GeneTex
- Proper citation
- GeneTex Cat#GTX101144, RRID:AB_1240971
- Product name
- HSC70 antibody
- Antibody type
- Polyclonal
- Reactivity
- Human, Mouse, Rat
- Host
- Rabbit
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Supportive validation
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- Sample (50 ?g of whole cell lysate) A: Mouse brain 7.5% SDS PAGE GTX101144 diluted at 1:5000 The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- Sample (30 ug of whole cell lysate) A: H1299 7.5% SDS PAGE GTX101144 diluted at 1:10000
- Validation comment
- WB
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- Various whole cell extracts (30 ?g) were separated by 7.5% SDS-PAGE, and the membrane was blotted with HSC70 antibody (GTX101144) diluted at 1:10000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- Various whole cell extracts (30 ?g) were separated by 7.5% SDS-PAGE, and the membrane was blotted with HSC70 antibody (GTX101144) diluted at 1:10000.
Supportive validation
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- Immunofluorescence analysis of methanol-fixed HeLa, using HSC70(GTX101144) antibody at 1:200 dilution.
Supportive validation
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- Immunohistochemical analysis of paraffin-embedded U87 xenograft, using HSC70(GTX101144) antibody at 1:100 dilution.
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- HSC70 antibody detects HSC70 protein at nucleolus on mouse prostate by immunohistochemical analysis. Sample: Paraffin-embedded mouse prostate. HSC70 antibody (GTX101144) dilution: 1:500.
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- HSC70 antibody detects HSC70 protein at nucleolus on rat hind brain by immunohistochemical analysis. Sample: Paraffin-embedded rat hind brain. HSC70 antibody (GTX101144) dilution: 1:500.
