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Western blotAntibody data
- Antibody Data
- Antigen structure
- References [3]
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- Validations
- Western blot [3]
- Immunocytochemistry [1]
- Immunohistochemistry [3]
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- Product number
- GTX111069 - Provider product page
- Provider
- GeneTex
- Proper citation
- GeneTex Cat#GTX111069, RRID:AB_1950526
- Product name
- HSC70 antibody [N2C1], Internal
- Antibody type
- Polyclonal
- Reactivity
- Human, Mouse
- Host
- Rabbit
Submitted references Exosomal secretion of α-synuclein as protective mechanism after upstream blockage of macroautophagy.
The regulation of heat shock proteins in response to dehydration in Xenopus laevis.
Activation of PI3K, Akt, and ERK during early rotavirus infection leads to V-ATPase-dependent endosomal acidification required for uncoating.
Fussi N, Höllerhage M, Chakroun T, Nykänen NP, Rösler TW, Koeglsperger T, Wurst W, Behrends C, Höglinger GU
Cell death & disease 2018 Jul 9;9(7):757
Cell death & disease 2018 Jul 9;9(7):757
The regulation of heat shock proteins in response to dehydration in Xenopus laevis.
Luu BE, Wijenayake S, Malik AI, Storey KB
Cell stress & chaperones 2018 Jan;23(1):45-53
Cell stress & chaperones 2018 Jan;23(1):45-53
Activation of PI3K, Akt, and ERK during early rotavirus infection leads to V-ATPase-dependent endosomal acidification required for uncoating.
Soliman M, Seo JY, Kim DS, Kim JY, Park JG, Alfajaro MM, Baek YB, Cho EH, Kwon J, Choi JS, Kang MI, Park SI, Cho KO
PLoS pathogens 2018 Jan;14(1):e1006820
PLoS pathogens 2018 Jan;14(1):e1006820
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Supportive validation
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- GeneTex (provider)
- Main image
- Experimental details
- Sample (30 ?g of whole cell lysate) A: Neuro2A B: GL261 C: C8D30 7.5% SDS PAGE GTX111069 diluted at 1:1000 The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- Sample (30 ?g of whole cell lysate) A: A431 (GTX27909) B: HeLa 7.5% SDS PAGE GTX111069 diluted at 1:1000 The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- HSC70 antibody detects HSC70 protein by western blot analysis.A. 30 ?g 293T whole cell lysate/extractB. 30 ?g A431whole cell lysate/extractC. 30 ?g HeLa whole cell lysate/extractD. 30 ?g HepG2 whole cell lysate/extract7.5% SDS-PAGEHSC70 antibody (GTX111069) dilution: 1:1000 The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
Supportive validation
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- HSC70 antibody detects HSC70 protein at cytoplasm by immunofluorescent analysis.Sample: HeLa cells were fixed in ice-cold MeOH for 5 min.Green: HSC70 protein stained by HSC70 antibody (GTX111069) diluted at 1:500.Blue: Hoechst 33342 staining.
Supportive validation
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- Immunohistochemical analysis of paraffin-embedded NCIN87 xenograft, using HSC70(GTX111069) antibody at 1:100 dilution.
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- HSC70 antibody detects HSC70 protein at cytosol on mouse liver by immunohistochemical analysis. Sample: Paraffin-embedded mouse liver. HSC70 antibody (GTX111069) dilution: 1:500.
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- HSC70 antibody detects HSC70 protein at cytosol on mouse prostate by immunohistochemical analysis. Sample: Paraffin-embedded mouse prostate. HSC70 antibody (GTX111069) dilution: 1:500.
