M01024-1

antibody from Boster Biological Technology

Targeting: HSPA8 HSC70, HSC71, HSP73, HSPA10

Western blot (Data presented on provider website)

Immunocytochemistry (Data presented on provider website)

Immunohistochemistry (Supportive data in Antibodypedia)

Flow cytometry (Data presented on provider website)

Antibody Data

Product number

M01024-1

Provider

Boster Biological Technology

Product name

Anti-Hsc70 Antibody Picoband™(monoclonal, 3B6)

Antibody type

Monoclonal

Description

Mouse IgG monoclonal antibody for Hsc70 detection. Tested with WB, IHC-P, ICC/IF, FCM in Human;Mouse;Rat.

Reactivity

Human, Mouse, Rat

Host

Mouse

Isotype

IgG

Antibody clone number

3B6

Vial size

100μg/vial

Storage

At -20ËšC for one year. After reconstitution, at 4ËšC for one month. It can also be aliquoted and stored frozen at -20ËšC for a longer time. Avoid repeated freezing and thawing.

Handling

Add 0.2ml of distilled water will yield a concentration of 500μg/ml.

Immunohistochemistry

Supportive validation

Submitted by

Boster Biological Technology (provider)

Main image

Experimental details

IHC analysis of Hsc70 using anti-Hsc70 antibody (M01024-1). Hsc70 was detected in paraffin-embedded section of human lung cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml mouse anti-Hsc70 Antibody (M01024-1) overnight at 4°C. Biotinylated goat anti-mouse IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1021) with DAB as the chromogen.

Additional image